Toward 3D luciferase imaging: bioluminescence tomography (BLT)
2008 is reportergenic! Take volume 13 of Frontiers in Bioscience of January 1, 2008. Ge Wang and colleagues of Virginia Tech, offer a nice overview of development of 3D bioluminescence tomography.
From pionieristic work in 2002, BLT aims to extend the 2D view of bioluminescent imaging toward a third direction: the deep. In such overview, the authors explains the history of bioluminescence imaging (BLI) from Berthold to Xenogen, elaborate on multispectral extensions of BLT and describes associated image unmixing and normalization techniques.