Reporters
pGreenFire 2.0 mCMV Cloning & Negative Control Lentivector & Virus
With the pGreenFire 2.0 mCMV vector you can add-in your own transcriptional response elements or use as-is as a negative control for any pGreenFire 2.0 project.
Make your own pGreenFire 2.0 reporter vector or use as a negative control
- With the pGreenFire 2.0 mCMV Cloning & Negative Control Lentivector and LentiVirus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro), you can take advantage of our robust pGreenFire 2.0 lentivector technology to create your own transcriptional response element (TRE) reporter or use pGreenFire 2.0 mCMV as-is as a negative control.
- The pGreenFire 2.0 mCMV Cloning & Negative Control Lentivector, XhoI and NheI sites are placed upstream of a minimal CMV promoter (mCMV) so you can clone in your own TREs. Upon activation, the TREs and mCMV promoter together drive co-expression of red firefly luciferase and GFP so you can quantitatively measure transcriptional activity using both fluorescence and luciferase activity.
- Alternatively, you can use the vector as-is as a negative control for any project using pGreenFire 2.0 lentivectors (see data below).
- What makes our next-gen pGreenFire 2.0 vectors even better than other TRE reporter vectors is the smart design, which adds in a constitutive selection cassette for stable cell line generation while minimizing interference with the upstream TRE. By using a weak/moderate mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and carefully arranging the conditional reporter genes, the selection marker is reliably expressed without compromising conditional expression of rFLuc and GFP.
- As with all of our pGreenFire 2.0 lentivectors, the GreenFire cassette now consists of red firefly luciferase (rFLuc), a T2A co-expression element, and GFP. The switch to rFLuc opens up the possibility of performing a dual-spectral luciferase assay and also delivers greater sensitivity for in vivo applications than conventional luciferase.
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pGreenFire 2.0-mCMV negative control virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€745.00pGreenFire 2.0-mCMV negative control virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR411VA-P. Supplier: SBI System Biosciences With the pGreenFire 2.0 mCMV vector you can add-in your own transcriptional response elements or use as-is as a negative...€745.00 -
pGreenFire 2.0-mCMV negative control plasmid (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€745.00pGreenFire 2.0-mCMV negative control plasmid (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR411PA-P. Supplier: SBI System Biosciences With the pGreenFire 2.0 mCMV vector you can add-in your own transcriptional response elements or use as-is as a negative...€745.00 -
pGreenFire 2.0-CMV positive control virus (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€745.00pGreenFire 2.0-CMV positive control virus (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR410VA-P. Supplier: SBI System Biosciences Use the pGreenFire 2.0 CMV Positive Control Lentivector as a ready-to-go positive control for any pGreenFire 2.0 project...€745.00 -
pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€745.00pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR410PA-P. Supplier: SBI System Biosciences Use the pGreenFire 2.0 CMV Positive Control Lentivector as a ready-to-go positive control for any pGreenFire 2.0 project...€745.00 -
pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro). Cat# TR413VA-P. Supplier: SBI System Biosciences Study TCF/LEF signaling with pGreenFire 2.0 TCF/LEF Reporter (red firefly luciferase & GFP) – specially engineered...€827.00 -
pGreenFire 2.0 TCF/LEF reporter plasmid (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 TCF/LEF reporter plasmid (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro). Cat# TR413PA-P. Supplier: SBI System Biosciences Study TCF/LEF signaling with pGreenFire 2.0 TCF/LEF Reporter (red firefly luciferase & GFP) – specially engineered...€827.00 -
pGreenFire 2.0 TCF/LEF clonal 293T reporter cell line (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€3,636.00pGreenFire 2.0 TCF/LEF clonal 293T reporter cell line (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro). Cat# TR413C-P. Supplier: SBI System Biosciences Study TCF/LEF signaling with pGreenFire 2.0 TCF/LEF Reporter (red firefly luciferase & GFP) –...€3,636.00 -
pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 NFkB reporter virus (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro). Cat# TR412VA-P. Supplier: SBI System Biosciences Study NF-κB signaling with the pGreenFire 2.0 NFκB Reporter (red firefly luciferase & GFP), which is engineered...€827.00 -
pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 NFkB reporter plasmid (pGF2-NFκB-rFluc-T2A-GFP-mPGK-Puro). Cat# TR412PA-P. Supplier: SBI System Biosciences Study NF-κB signaling with the pGreenFire 2.0 NFκB Reporter (red firefly luciferase & GFP), which is...€827.00 -
pGreenFire 2.0 NFAT reporter virus (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 NFAT reporter virus (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro). Cat# TR451VA-P. Supplier: SBI System Biosciences Study NFAT signaling with this next-gen pGreenFire 2.0 NFAT Reporter (red firefly luciferase & GFP) that is engineered for...€827.00 -
pGreenFire 2.0 NFAT reporter plasmid (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 NFAT reporter plasmid (pGF2-NFAT-rFluc-T2A-GFP-mPGK-Puro). Cat# TR451PA-P. Supplier: SBI System Biosciences Study NFAT signaling with this next-gen pGreenFire 2.0 NFAT Reporter (red firefly luciferase & GFP) that is engineered for...€827.00 -
pGreenFire 2.0 HIF-1 reporter virus (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 HIF-1 reporter virus (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR426VA-P. Supplier: SBI System Biosciences Study HIF-1 signaling with the pGreenFire 2.0 HIF-1 Reporter (red firefly luciferase & GFP) which is engineered for reliable...€827.00 -
pGreenFire 2.0 HIF-1 reporter plasmid (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 HIF-1 reporter plasmid (pGF2-HIF1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR426PA-P. Supplier: SBI System Biosciences Study HIF-1 signaling with the pGreenFire 2.0 HIF-1 Reporter (red firefly luciferase & GFP) which is engineered for reliable...€827.00 -
pGreenFire 2.0 Estrogen response element reporter virus (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 Estrogen response element reporter virus (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro). Cat# TR455VA-P. Supplier: SBI System Biosciences Study estrogen signaling with this next-gen pGreenFire 2.0 lentivector (red firefly luciferase & GFP), which...€827.00 -
pGreenFire 2.0 Estrogen response element reporter plasmid (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 Estrogen response element reporter plasmid (pGF2-ERE-rFluc-T2A-GFP-mPGK-Puro). Cat# TR455PA-P. Supplier: SBI System Biosciences Study estrogen signaling with this next-gen pGreenFire 2.0 lentivector (red firefly luciferase & GFP),...€827.00 -
pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 AP-1 reporter virus (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR452VA-P. Supplier: SBI System Biosciences Study oncogenic signaling with the next-gen pGreenFire 2.0 AP-1 Reporter (red firefly luciferase & GFP), engineered for reliable...€827.00 -
pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€827.00pGreenFire 2.0 AP-1 reporter plasmid (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR452PA-P. Supplier: SBI System Biosciences Study oncogenic signaling with the next-gen pGreenFire 2.0 AP-1 Reporter (red firefly luciferase & GFP), engineered for reliable...€827.00 -
pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro)
System Biosciences
€3,636.00pGreenFire 2.0 AP-1 clonal 293T reporter cell line (pGF2-AP1-rFluc-T2A-GFP-mPGK-Puro). Cat# TR452C-P. Supplier: SBI System Biosciences Study oncogenic signaling with the next-gen pGreenFire 2.0 AP-1 Reporter (red firefly luciferase & GFP), engineered...€3,636.00