System Biosciences

pGreenFire1-HIF1 (virus) + EF1-Neo

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SKU:: TR026VA-N
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Description

Description

pGreenFire1-HIF1 (virus) + EF1-Neo. Cat# TR026VA-N. Supplier: SBI System Biosciences

Study HIF1 signal transduction by co-expressing dscGFP and luciferase in response to HIF1 activity (includes EF1α-neo selection cassette)

Sort responsive cells with dscGFP
Measure activity with luciferase
Leverage SBI’s highly-regarded lentivectors
Create stable signaling pathway reporter cell lines
Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

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Overview

Monitor signal transduction in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-HIF1 (EF1α-neo) Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from HIF1 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV).

The mCMV promoter alone delivers negligible expression, but when downstream of HIF1-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to HIF1 activity. The result is the ability to quantitatively measure HIF1 activity by fluorescence and luciferase activity.

Sort responsive cells with dscGFP
Measure activity with luciferase
Leverage SBI’s highly-regarded lentivectors
Create stable signaling pathway reporter cell lines
Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

To facilitate cell line construction, the pGreenFire1-HIF1 (EF1α-neo) Lentivector comes with a constitutively-expressed neomycin marker from the EF1α promoter. Or you can choose a puromycin marker (EF1α-neo; Cat.# TR026PA-P) or no marker at all (Cat.# TR026PA-1). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examples

Monitoring NF-κB transactivation

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