System Biosciences
pGreenFire1-Nanog (plasmid)+ EF1-Puro
- SKU:
- TR019PA-P
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
pGreenFire1-Nanog (plasmid)+ EF1-Puro. Cat# TR019PA-P. Supplier: SBI System Biosciences
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
Products
Overview
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
To facilitate cell line construction, the pGreenFire1-Nanog (EF1α-puro) Lentivector comes with a constitutively-expressed puromycin marker from the EF1α promoter. Or you can choose a neomycin marker (EF1α-neo; Cat.# TR019PA-N) or no marker at all (Cat.# TR019PA-1). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.
*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.Supporting Data
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation
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