pGreenFire1-Notch (plasmid)+ EF1-Puro

Monitor signal transduction in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-Notch (EF1α-puro) Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from Notch transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV).

 

The mCMV promoter alone delivers negligible expression, but when downstream of Notch-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to Notch activity. The result is the ability to quantitatively measure Notch activity by fluorescence and luciferase activity.

• Sort responsive cells with dscGFP
• Measure activity with luciferase
• Leverage SBI’s highly-regarded lentivectors
• Create stable signaling pathway reporter cell lines
• Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

To facilitate cell line construction, the pGreenFire1-Notch (EF1α-puro) Lentivector comes with a constitutively-expressed puromycin marker from the EF1α promoter. Or you can choose a neomycin marker (EF1α-neo; Cat.# TR020PA-N) or no marker at all (Cat.# TR020PA-1). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.