System Biosciences

pGreenZeo-CMV Virus [positive control]

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SKU:: SR501VA
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Description

Description

pGreenZeo-CMV Virus [positive control]. Cat# SR501VA. Supplier: SBI System Biosciences

Run your pGreenZeo projects with confidence with the addition of this positive control that drives copGFP and zeomycin resistance with a CMV promoter

Products

Overview

Supporting your studies with ready-to-go positive controls No need to make a positive control for your pGreenZeo projects—SBI’s already built one for you. The GreenZeo cassette is driven by the strong, constitutive CMV promoter with the GFP and zeomycin marker co-expression mediated by a T2A element.Choose the differentiation reporter that’s right for youNeural differentiation reporters

Hematopoietic differentiation reporters

Structural differentiation reporters

Supporting Data

See some of our differentiation reporters in action

SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human Nestin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonsthumaning the specificity of SBI’s human Nestin pGreenFire Differentiation Reporter.

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

Figure 3. Additional differentiation reporter data.

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