pLL-EF1a-BFP-T2A-Puro [Lenti-Labeler™ plasmid]

Reliable cell labeling, delivered SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-EF1α-BFP-T2A-Puro Lenti-Labeler™ construct expresses BFP from the EF1α promoter, which delivers moderate expression in most cell types (including primary and stem cells), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL220PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL220VA-1), SBI’s pLL-EF1α-BFP-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster

 

• Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
• Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
• Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)

While the pLL-EF1α-BFP-T2A-Puro Lenti-Labeler construct is compatible with most second and third generation packaging plasmid mixes, SBI recommends the use of pPACKH1 (Cat #LV500A-1) and TransDux MAX Transduction Reagent (Cat #LV860A-1) to achieve optimal virus titers and infection of target cells.Find the right Lenti-Labeler construct for your studies

SBI offers Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.

Table 1. Available Lenti-Labeler Constructs