RAWS 264.7 Mouse macrophage cell line: >1x10^6 frozen exosomes

Validated using Western blotting

All exosome preps are checked for the presence of CD63, a common exosome marker, via Western blotting (Figure 1).

Figure 1. All exosome preps contain CD63. Aliquots of purified exosomes from the cell lines and from human serum were lysed with either RIPA or M-PER buffer to make exosome protein lysates. Approximately 20 ug of protein for each sample was separated on a gradient SDS-PAGE and then transferred to nitrocellulose membranes. The membranes were probed for CD63 profiles using SBI’s anti-CD63 antibody (cat# EXOAB-CD63A-1) at a 1:1,000 dilution. Bands were detected using the secondary HRP-conjugated antibody at 1:10,000 and blots imaged. All purified exosome preparations were positive, immunoreactive for CD63 with the expected, variable banding patterns common to published exosome CD63 profiles.