XMIR Texas-Red labeled positive control RNA oligo with Xmotif

The XMIR Texas Red-labeled Positive Control Oligo in action

Figure 1. The XMIR Texas Red-labeled Positive Control Oligo is efficiently delivered to recipient cells by exosomes. METHODS: HEK-293 cells were transfected with the XMIR Texas Red-labeled Positive Control Oligo. Twenty-four hours later, exosomes were isolated using ExoQuick-TC® and added to naïve HEK293 target cells. After 4-hours, cells were imaged on a Leica DMI300B fluorescence microscope using a standard RFP filter set to visualize the Texas-Red signal.

Figure 2. The abundance of the XMIR Texas Red-labeled Positive Control Oligo in recipient cells can be measured using qPCR. METHODS: The recipient HEK-293 cells from Figure 1 were lysed and total cellular RNA extracted in preparation for qPCR. cDNA synthesis and qPCR analysis were performed using using SBI’s QuantiMir kit (Cat.# RA420A-1) and the qPCR assay run on an Applied Biosystems 7900HT qPCR machine using the spike-in forward primer to detect abundance of the positive control oligo. Abundance was normalized to endogenous reference miR-16 levels and calculated using the ΔΔC_t method, and clearly show good transfer of the XMIR Positive Control Oligo to the recipient cells.