- Home
- Gene Editing
- AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
- SKU:
- GE624A-KIT
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 1 kit
- Shipping Temperature:
- RT/Blue Ice/ Dry Ice
Description
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site). Cat# GE620A-KIT. Supplier: SBI System Biosciences
- Easy, precise knock-in of any gene
- Consistent, robust transgene expression from the AAVS1 Safe Harbor Site
- Simplified construction of isogenic cell lines
- Minimal off-target integration
- Streamlined CRISPR/Cas9 library screening
Products

Overview

- Easy, precise knock-in of any gene
- Consistent, robust transgene expression from the AAVS1 Safe Harbor Site
- Simplified construction of isogenic cell lines
- Minimal off-target integration
- Streamlined CRISPR/Cas9 library screening
How It Works
Gene knock-in at AAVS1
Figure 1. Knocking-in a gene at the AAVS1 site using an HR Targeting Vector.
Step 1: Cas9 creates a double-stranded break(DSB) at the AAVS1 site. Cas9 activity is directed to the AAVS1 site by an AAVS1-specific gRNA.
Step 2: The DNA repair machinery is recruited to the DSB. In the presence of an HR Donor with homology to the region adjacent to the DSB (blue areas of the genomic and plasmid DNA) homologous recombination (HR) is favored over non-homologous end joining (NHEJ).
Result: The HR event leads to insertion of the region of the HR Donor Vector between the two homology arms—your gene-of-interest is integrated into the AAVS1 site.
Genome engineering with CRISPR/Cas9
For general guidance on using CRISPR/Cas9 technology for genome engineering, take a look at our CRISPR/Cas9 tutorials as well as the following application notes:
CRISPR/Cas9 Basics
Through careful selection of the target sequence and design of a donor plasmid for homologous
recombination, you can achieve efficient and highly targeted genomic modification with CRISPR/Cas9.
The system
Cas9 protein—uses guide RNA (gRNA) to direct site-specific, double-strand DNA cleavage adjacent to a protospacer adapter motif (PAM) in the target DNA.
gRNA—RNA sequence that guides Cas9 to cleave a homologous region in the target genome. Efficient cleavage only where the gRNA homology is adjacent to a PAM.
PAM—protospacer adapter motif, NGG, is a target DNA sequence that spCas9 will cut upstream from if directed to by the gRNA.
The workflow at-a-glance
DESIGN: Select gRNA and HR donor plasmids. Choice of gRNA site and design of donor
plasmid determines whether the homologous recombination event results in a knock-out,
knock-in, edit, or tagging.
CONSTRUCT: Clone gRNA into all-in-one Cas9 vector. Clone 5’ and 3’ homology arms into HR
donor plasmid. If creating a knock-in, clone desired gene into HR donor.
CO-TRANSFECT or CO-INJECT: Introduce Cas9, gRNA, and HR Donors into the target cells
using co-transfection for plasmids, co-transduction for lentivirus, or co-injection for mRNAs.
SELECT/SCREEN: Select or screen for mutants and verify.
VALIDATE: Genotype or sequence putative mutants to verify single or biallelic conversion.
Related Products


AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences


hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1
System Biosciences
Customers Also Viewed


A549 Human non-small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences

3-min Detergent-free Total Protein Extraction Kit (Animal Cells) | 568-P505
101Bio


AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences
![AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro] AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12431/12695/sbi%20system%20biosciences_1633075021__00205.original__65072.1633076526.jpg?c=1)
![AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro] AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12431/15410/PIN610A-1__11136.1638689071.png?c=1)
AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro]
System Biosciences


AAVS1 Positive Control EGIP 293T Reporter Cell Line
System Biosciences


"N" SARS-CoV-2 Nucleocapsid mRNA (10 ug, Transfection-ready)
System Biosciences

All-Lab Water Bath Treatment Tablet (1 tablet / treatment of 1 – 2 Gal. / week) | 568-T20X
101Bio

3-min Detergent-free Total Protein Extraction Kit (Animal Tissues) | 568-P506
101Bio


Anti-Vimentin Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-FLOT1 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-EpCam Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-CD63 Antibody (rabbit anti-human) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-ANXA5 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-ALIX Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)
System Biosciences


AAVanced™ Concentration Reagent (250 ml aliquot)
System Biosciences


AAVanced™ Concentration Reagent (100 ml aliquot)
System Biosciences


5’ and 3’ AAVS1 Positive Control Donor Vector Primer Mixes for Junction PCR Assays (10
System Biosciences

"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 ug, Transfection-ready)
System Biosciences

8-min Intact Plant Chloroplast Isolation Kit | 568-P511L
101Bio

Plasma Membrane Protein Extraction Kit (cell/tissue) | 568-P503
101Bio

sRNA-into-Exosome Kit (Chemical) | 568-P401
101Bio

Sample of P201 | 568-P201S
101Bio

Detergent-free Exosomal Protein Extraction Kit | 568-P201
101Bio

Exosomal RNA Extraction Kit | 568-P200R, part of P200
101Bio

Exosome Isolation kit (forHuman Body Fluid) | 568-P121X
101Bio


XStamp MLN exosome Motilin receptor GI tract targeting lentivector
System Biosciences


SmartSEC™ Mini EV Isolation System
System Biosciences


Purified Exosomes from Human Urine (Healthy Donors)
System Biosciences


Purified Exosomes from Human Serum (Healthy Donors)
System Biosciences


Purified Exosomes from Human Saliva (Healthy Donors)
System Biosciences


PEG-it Virus Precipitation Solution (100 mL aliquot)
System Biosciences


Exo-Urine EV Isolation Kit
System Biosciences


ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
System Biosciences


ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
System Biosciences


ExoQuick-LP for lipoprotein pre-clear & exosome isolation
System Biosciences


ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
System Biosciences


ExoQuick PLUS Exosome Purification Kit - for serum and plasma
System Biosciences


ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL)
System Biosciences


ExoQuick Exosome RNA Column Purification kit
System Biosciences


ExoQuick exosome precipitation solution (5 ml)
System Biosciences


ExoQuick exosome precipitation solution (20 ml)
System Biosciences


ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
System Biosciences


Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
System Biosciences


Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid
System Biosciences
![Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12513/12777/sbi%20system%20biosciences_1633075021__00205.original__91910.1633076539.jpg?c=1)
![Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12513/15551/CASLV225PA-1__24299.1638864340.png?c=1)
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]
System Biosciences