System Biosciences

Anti-FLOT1 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody

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SKU:
EXOAB-FLOT1-1
Availability:
Usually Shipped in 5 Working Days
Size:
25 ul
Shipping Temperature:
Blue Ice
  • Anti-FLOT1 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
  • Anti-FLOT1 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
€194.00

Description

Anti-FLOT1 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody. Cat# EXOAB-FLOT1. Supplier: SBI System Biosciences

Detect exosomes using SBI’s rigorously validated polyclonal rabbit anti-FLOT1 antibodies that recognize the human, rat, and mouse versions of the protein.
  • Rigorously validated
  • Hand-picked selection
  • Expert exosome support

Products

Overview

Confirm exosome isolation with confidence

When you’re ready to confirm that you’ve isolated exosomes, stick with the exosome experts at SBI. Our polyclonal rabbit anti-FLOT1 antibodies recognize the human, mouse, and rat forms of the protein, and are a great choice for general exosome detection, especially when used in conjunction with antibodies for other exosome markers such as the tetraspanins CD9, CD63, and CD81, as well as the heat shock protein HSP70 (if you have any questions about the best antibodies to use for your exosome project, we’re ready to help! Just email us at tech@systembio.com). And, like all of our antibodies, our anti-FLOT1 antibody and the included goat anti-rabbit HRP secondary antibodies are rigorously validated for exosome detection via Western blotting.

  • Rigorously validated
  • Hand-picked selection
  • Expert exosome support

Choose the right antibody for your exosome detection needs

Supporting Data

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.

Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.

Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.

 

 

 

 

 
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