System Biosciences
AXMIR-let7a RNA oligo anti-miRNA-let7a-5p with Xmotif
- SKU:
- AXMIR-let7a
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 reactions
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
AXMIR-let7a RNA oligo anti-miRNA-let7a-5p with Xmotif. Cat# AXMIR-let7a. Supplier: SBI System Biosciences
Use exosomes to deliver anti-miRNAs to cells: with our ready-to-transfect AXMIR RNA Oligos, you can easily generate exosomes containing a specific anti-miRNA.
Overview
Putting exosomes to work: Delivering anti-miRNA
With SBI’s unique AXMIR (and the related XMIR) technology, you can take advantage of normal cellular processes to package a specific anti-miRNA into exosomes, and then use those exosomes to deliver that anti-miRNA to target cells. Choose from one of our pre-built, ready-to-transfect AXMIRs (see list below). If you don’t see the anti-miRNA you’re interested in, simply contact us at tech@systembio.com and we’ll design and synthesize the custom AXMIR for you.
- Flexible—generate anti-miRNA-packed exosomes using the cell line of your choice
- Powerful—great for a range of applications, including:
- Modulating expression of miRNA-targets in recipient cells
- Developing exosome-delivered anti-miRNA-based therapies
How It Works
Anti-miRNA packaging into exosomes with AXMIR
AXMIR packaging relies on an RNA sequence tag—the XMotif (identified and optimized by SBI)—that targets small RNAs to exosomes for packaging. By fusing the XMotif to an anti-miRNA oligo, you can ensure packaging of that anti-miRNA into exosomes. Simply transfect an AXMIR Oligo into exosome-producing cells, wait a short time for new exosome packaging, and isolate newly secreted exosomes containing your anti-miRNA.
Supporting Data
XMIRs and AXMIRs can be used to modulate levels of a specific protein in target cells
With XMIR/AXMIR technology, you can use exosomes to deliver miRNAs and anti-miRNAs to cells in order to alter levels of a specific protein (NOTE: when combined with our XStampTM Exosome Targeting Technology, you can even generate miR/anti-miR-carrying exosomes that are targeted to a specific cell type).
To demonstrate the effectiveness of using exosomes containing either miRNA or anti-miRNA to modulate protein expression levels in target cells, we generated XMIR-21 and AXMIR-21 containing exosomes from HEK293 cells, and used Western blotting to monitor expression levels of the protein PDCD4, whose expression is reduced in the presence of miR-21.
We transfected HEK293 cells with either an miR-21 XMIR Oligo (XMIR-21) or an anti-miR-21 AXMIR Oligo (AXMIR-21), isolated exosomes loaded with the respective XMIR/AXMIR, and then added these exosomes to naïve HEK-293 cells in culture. After 24 hours, total cell lysates were taken and Western blots for PDCD4, a known miR-21 target, were performed (Figure 1A), with GAPDH protein levels used as a loading control and reference signal for band intensity quantitation analysis. Quantitation is shown in Figure 1B.
These data clearly show that XMIR and AXMIR-containing exosomes can modulate expression of the target protein, as addition of exosomes from cells transfected with XMIR-21 resulted in down regulation of endogenous levels of PDCD4, and addition of exosomes from cells transfected with AXMIR-21 resulted in increased levels of PDCD4. These results also confirm that XMIRs act on endogenous targets and that AXMIRs effectively act as miRNA inhibitors in cells when delivered via exosomes.
Figure 1. XMIR-21 knockdown and AXMIR-21 upregulation of the miR-21 target PDCD4. (A) Western blot data and (B) quantitation.
Related Products


AXMIR-125b RNA oligo anti-miRNA-125b-5p with Xmotif
System Biosciences


AXMIR-21 RNA oligo anti-miRNA-21-5p with Xmotif
System Biosciences


AXMIR-219 RNA oligo anti-miRNA-219a-5p with Xmotif
System Biosciences

AXMIR-31 RNA oligo anti-miRNA-31-5p with Xmotif
System Biosciences


XMIR-let7a RNA oligo miRNA-let7a-5p with Xmotif
System Biosciences
Customers Also Viewed


Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid
System Biosciences


Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
System Biosciences


CAG-T7-hspCas9-T2A-RFP-H1-gRNA linearized SmartNuclease vector
System Biosciences


Anti-CETP Antibody (polyclonal rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-CD9 Antibody (rabbit anti-human) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-CD81 Antibody (rabbit anti-human) with goat anti-rabbit HRP secondary antibody
System Biosciences


Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA linearized all-in-one SmartNuclease Lentivector Plasmid
System Biosciences
![Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12516/12780/sbi%20system%20biosciences_1633075021__00205.original__96364.1633076539.jpg?c=1)
![Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12516/15553/CASLV225PA-1__70182.1638864794.png?c=1)
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]
System Biosciences


Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid
System Biosciences


Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
System Biosciences


Cas9 Nickase: CMV-T7-hspCas9-nickase-T2A-RFP-H1-gRNA linearized SmartNickase vector
System Biosciences


Cas9 Nickase: CMV-T7-hspCas9-nickase-T2A-GFP-H1-gRNA linearized SmartNickase vector
System Biosciences


Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
System Biosciences


Cas9 Nickase: CMV-hspCas9-nickase-H1-gRNA SmartNickase vector
System Biosciences
![Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12501/12765/sbi%20system%20biosciences_1633075021__00205.original__53274.1633076537.jpg?c=1)
![Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs] Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12501/15496/CASLV205PA-1__77169.1638786899.png?c=1)
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Pre-Packaged Lentiviral Particles [>10^6 IFUs]
System Biosciences

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid
System Biosciences


Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
System Biosciences


Cas9 Nickase: CAG-hspCas9-nickase-H1-gRNA SmartNickase vector
System Biosciences


CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
System Biosciences


CAGs-GFP-T2A-Luciferase Dual Reporter Enhanced Episomal Vector (EEV), 10 ug (Replaces EEV604A-1)
System Biosciences


CAG PinPoint Donor Vector (pPP-CAG-MCS-WPRE-pA-attB-Puro-pA)
System Biosciences


BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Pre-packaged Virus
System Biosciences


BLIV 2.0 Reporter: CMV-Luciferase-EF1a-copGFP Pre-packaged Virus
System Biosciences

AXMIR-34a RNA oligo anti-miRNA-34a-5p with Xmotif
System Biosciences

AXMIR-31 RNA oligo anti-miRNA-31-5p with Xmotif
System Biosciences


AXMIR-29a RNA oligo anti-miRNA-29a-3p with Xmotif
System Biosciences


AXMIR-223 RNA oligo anti-miRNA-223-3p with Xmotif
System Biosciences


AXMIR-221 RNA oligo anti-miRNA-221-3p with Xmotif
System Biosciences


AXMIR-146a RNA oligo anti-miRNA-146a-5p with Xmotif
System Biosciences


AXMIR-145 RNA oligo anti-miRNA-145-5p with Xmotif
System Biosciences


AXMIR-125b RNA oligo anti-miRNA-125b-5p with Xmotif
System Biosciences


AXMIR-125a RNA oligo anti-miRNA-125a-5p with Xmotif
System Biosciences


AXMIR-124a RNA oligo anti-miRNA-124a-3p with Xmotif
System Biosciences


Arabinose induction solution (20%)
System Biosciences


Anti-Vimentin Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-Hsp70 Antibody (rabbit anti-human) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-FLOT1 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-EpCam Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


Anti-ANXA5 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody
System Biosciences


ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Pre-packaged Lentivirus)
System Biosciences


ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)
System Biosciences


AAVanced™ Concentration Reagent (250 ml aliquot)
System Biosciences


AAVanced™ Concentration Reagent (100 ml aliquot)
System Biosciences


293TN Producer Cell Line
System Biosciences

"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 ug, Transfection-ready)
System Biosciences


"N" SARS-CoV-2 Nucleocapsid mRNA (20 ug, Transfection-ready)
System Biosciences