System Biosciences

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid

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SKU:
BLIV713PA-1
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Size:
10 ug
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  • BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid
  • BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid
£731.86

Description

BLIV 2.0 Reporter: MSCV-Luciferase-EF1a-copGFP-T2A-Puro Lentivector Plasmid. Cat# BLIV713PA. Supplier: SBI System Biosciences

Use this BLIV 2.0 vector to track cell fate after implantation, or other in vivo imaging applications – delivers CMV-driven Luciferase and EF1α-driven copGFP
  • You’re working with difficult-to-transfect cells
  • You’d like to create stable reporter cell lines
  • You’d like to integrate your reporter into the genome
  • You’d like episomal expression
  • You’d like to avoid introducing foreign sequences

Products

Overview

Clearly visualize cells in vivo and ex vivo for tracking cell fate and more
 
 Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression. SBI's lentivector-based imaging systems offer safe, stable, and effective gene delivery in virtually all mammalian cells in tissue culture and in whole animal models
 
The CMV-Luciferase-EF1α-copGFP BLIV 2.0 Lentivector for In Vivo Imaging leverages SBI’s well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. The 2.0 BLIVs have been further engineered to provide enhanced performance and reliability than the first-generation vectors. Note that lentivectors can be used to generate stable reporter cell lines as they integrate into the genome. The CMV-Luciferase-EF1α-copGFP vector expresses luciferase from a CMV promoter for high expression in most cell types, and the bright copGFP from the EF1α promoter for medium expression. Available as either a plasmid or pre-packaged virus.
 
 Which BLIV construct should you choose?
 
 With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs: Choose a lentivector when:
  • You’re working with difficult-to-transfect cells
  • You’d like to create stable reporter cell lines
  • You’d like to integrate your reporter into the genome
Choose a minicircle vector* when you’re working with transfectable cells and:
  • You’d like episomal expression
  • You’d like to avoid introducing foreign sequences
Choose your promoter using the following table:
 
Note that Minicircle Parental Vectors are only available to academic/non-profit customers. Researchers at commercial organization may only purchase the pre-made minicircle BLIVs.

Supporting Data

See SBI’s BLIV Vectors in action

Figure 1. Examples of how SBI’s BLIV lentivectors deliver strong luciferase activity after both transduction and transfection.

Figure 2. Examples of how SBI’s BLIV minicircle vectors deliver strong luciferase activity in small animal models.

Figure 3. Examples of how SBI’s BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity.

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