EGFP-labeled Cas9 Protein (NLS-Cas9-EGFP)

Experience all the benefits of purified Cas9 protein with the added ability to screen for successful introduction into target cells with the EGFP-labeled Cas9 Protein (NLS-Cas9-EGFP). Highly concentrated and ready to use, the NLS-Cas9-EGFP protein is ready for introduction into target cells via transfection, electroporation, or microinjection. Once inside the cell, a nuclear localization signal (NLS) directs the protein to the nucleus where it catalyzes gRNA-directed cleavage before being rapidly cleared.

NLS-Cas9-EGFP protein enables efficient genome editing while reducing off-target events^1–3. By removing plasmid delivery of Cas9 from the genome editing process, both off-target events from random plasmid integration and the potential for an immune response from bacterial plasmid sequences are avoided^1,2. In addition, the more transient nature of Cas9 protein compared to plasmid or mRNA delivery further reduces off-target activity without decreasing on-target efficiency^1,2. The end result is better, safer Cas9 activity for applications where off-target events need to be minimized, such as:

• Genome engineering in embryos
• Disease model generation of organisms and cell lines
• In vitro transfection of cells
• In vitro cleavage assays for functional gRNA screens

• Increase Cas9 efficiency²
• Reduce off-target events²
• Reduce the potential for immune response to vector DNA
• Simplify delivery to cells and embryos
• Perform multiplex, high-throughput studies
• Conduct typical downstream functional assays

Streamline genome editing with transfectable/electroporatable Cas9 protein—get Cas9 protein bundled into a kit with the Cas9 Protein & T7 gRNA SmartNuclease™ Synthesis Kit (Cat.# CAS420A-KIT) .

Not sure whether you need a CRISPR/Cas9 plasmid, purified protein, or mRNA? Use this table to choose the CRISPR/Cas9 product that’s right for you: