System Biosciences
EGFP-labeled Cas9 Protein (NLS-Cas9-EGFP)
- SKU:
- CAS420A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 50 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
EGFP-labeled Cas9 Protein (NLS-Cas9-EGFP) . Cat# CAS420A. Supplier: SBI System Biosciences
Products
Overview
Experience all the benefits of purified Cas9 protein with the added ability to screen for successful introduction into target cells with the EGFP-labeled Cas9 Protein (NLS-Cas9-EGFP). Highly concentrated and ready to use, the NLS-Cas9-EGFP protein is ready for introduction into target cells via transfection, electroporation, or microinjection. Once inside the cell, a nuclear localization signal (NLS) directs the protein to the nucleus where it catalyzes gRNA-directed cleavage before being rapidly cleared.
NLS-Cas9-EGFP protein enables efficient genome editing while reducing off-target events1–3. By removing plasmid delivery of Cas9 from the genome editing process, both off-target events from random plasmid integration and the potential for an immune response from bacterial plasmid sequences are avoided1,2. In addition, the more transient nature of Cas9 protein compared to plasmid or mRNA delivery further reduces off-target activity without decreasing on-target efficiency1,2. The end result is better, safer Cas9 activity for applications where off-target events need to be minimized, such as:
- Genome engineering in embryos
- Disease model generation of organisms and cell lines
- In vitro transfection of cells
- In vitro cleavage assays for functional gRNA screens
- Increase Cas9 efficiency2
- Reduce off-target events2
- Reduce the potential for immune response to vector DNA
- Simplify delivery to cells and embryos
- Perform multiplex, high-throughput studies
- Conduct typical downstream functional assays
Streamline genome editing with transfectable/electroporatable Cas9 protein—get Cas9 protein bundled into a kit with the Cas9 Protein & T7 gRNA SmartNuclease™ Synthesis Kit (Cat.# CAS420A-KIT) .
Not sure whether you need a CRISPR/Cas9 plasmid, purified protein, or mRNA? Use this table to choose the CRISPR/Cas9 product that’s right for you:
Related Products

pET- NLS- Cas9- 6xHis | PVT10639
Nova Lifetech

pST1374- NLS- flag- linker- Cas9 | PVT11680
Nova Lifetech


Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
System Biosciences


Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit
System Biosciences


Purified Cas9 Protein (NLS-Cas9-NLS)
System Biosciences
Customers Also Viewed
![Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12523/12787/sbi%20system%20biosciences_1633075021__00205.original__98344.1633076540.jpg?c=1)
![Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12523/15418/Screenshot_2021-12-05_145530__76330.1638698497.png?c=1)
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
System Biosciences

Retro-SV40LT Retrovirus | RVP010
ABM Good

Lenti-HOXA10-RFP Lentivirus | LV647
ABM Good

Lenti-HOXA10-C-term-HA Lentivirus, High Titer | LV648
ABM Good

Lenti-HOXA10-C-term-HA Lentivirus | LV645
ABM Good

Lenti-EF1a-hTERT-Hygro Virus, High Titer | LV632
ABM Good

Lenti-EF1a-hTERT-Hygro Virus | LV631
ABM Good

Lenti-EF1α-hTERT Virus, High Titer | LV616
ABM Good

Lenti-EF1α-hTERT Virus | G706
ABM Good

Lenti-CMV-hTERT-RFP-2A-Puro Virus | G268
ABM Good

Lenti-CDK4 Virus | LV611
ABM Good

E1A-E1B Lentivirus | LV651
ABM Good

Adeno-SV40 Adenovirus | G210
ABM Good

Adeno-hTERT Antisense | G208
ABM Good


T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
System Biosciences


Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus
System Biosciences


Purified Cas9 Protein (NLS-Cas9-NLS)
System Biosciences


PrecisionX Multiplex gRNA Cloning Kit
System Biosciences


hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1
System Biosciences


hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)
System Biosciences


EF1-RFP-U6-gRNA(SA) linearized SmartNuclease AAV Plasmid
System Biosciences


EF1a-hsaCas9 SmartNuclease AAV Plasmid
System Biosciences


CLOuD9 Inducer Reagent (0.5M) - 400 ul
System Biosciences


CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)
System Biosciences


Cas9 RT-PCR Primer Set (50ul at 5uM for each primer set)
System Biosciences