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System Biosciences

EXOCET Standards

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SKU:
EXOCET-SD-1
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  • EXOCET Standards
  • EXOCET Standards
€174.00

Description

EXOCET Standards. Cat# EXOCET-SD. Supplier: SBI System Biosciences

Overview

Extra standards for EXOCET

While our fast and easy EXOCET Kit comes with standards, we also offer our EXOCET exosome standards as a stand-alone product for when you need more. The standards are calibrated using NanoSight analysis, enabling estimation of the number of exosomes in your samples.

The EXOCET Exosome Quantitation Kit is:

  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Choose the exosome quantitation method that’s best for your studies

How It Works

Exosome quantitation with EXOCET is quick and easy

  1. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  2. Lyse exosomes with the included Exosome Lysis Buffer
  3. Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm

 

Supporting Data

The fast, highly quantitative EXOCET exosome quantitation assay

Exosome quantitation with EXOCET takes as little as 20 minutes and 50 µg (protein equivalent) of sample. Exosomes were isolated from either human serum (0.5 mL) or from MDA-MB-231 culture media (10 mL) using standard ExoQuick and ExoQuick-TC reagents. Exosome pellets were resuspended in PBS and total protein concentration measured using a BCA assay (~108 exosomes, 2 µg/µL). Exosomes were lysed using the EXOCET gentle lysis solution to preserve the enzymatic activity of the exosomal AChE enzyme. The standard curve was generated using known numbers of exosomes (as measured by NanoSight) and calibrated with a recombinant AChE enzyme standard solution provided in the kit.

 

 
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