System Biosciences
LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 10 preps
- SKU:
- LV340A-1
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 preps
- Shipping Temperature:
- Blue Ice
Description
LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 10 preps. Cat# LV340A. Supplier: SBI System Biosciences
Overview
Get the reagents you need for large scale lentivirus production efficiently bundled into a single kit with the LentiSuite Basic Kit. This kit is ideal for fast-moving labs that don’t need quantitative titer information, and supports generation of high-titer lentivirus particles for any application where stable gene delivery and expression is desired.
Compatible with all of SBI’s lentivectors and constructs, as well as all other third-generation lentivectors, the LentiSuite Basic Kit includes optimized reagents to support large-scale virus production—you provide the HEK293T/TN/FT producer cells and lentivector (or purchase them separately) and this kit provides enough critical reagents to support 10 virus preps from 10 cm plates, or 5 virus preps from 15 cm plates:
- pPACK Packaging System (200 µL)
- PureFection Transfection Reagent (1 mL)
- PEG-it Concentration Reagent (100 mL)
- TransDux™ MAX Transduction Reagent (100 reactions*)
- SBI’s well-proven, widely-published lentivirus technology
- Optimized reagents that we keep on improving
- Deep technical expertise from the scientists that develop our lentivirus production products and perform our lentivirus production services
How It Works
For fast-moving labs generating lentivirus at large scales
Kit Contents
pPACK Packaging System
Reliable—generate high titers of transduction-ready pseudoviral particles
Powerful—efficiently deliver and stably express your lentivector constructs in a wide range of mammalian cells
Well-designed—quantify pseudoviral titers and transduction efficiency using a convenient copGFP control
Flexible—choose from FIV and HIV-based lentivector packaging kits
PureFection Transfection Reagent
Flexible—highly effective transfection technology supports a range of applications
Compatible with most cell types
Works with both plasmid DNA and siRNAs
Cost-effective—excellent alternative to expensive, lipid-based products
Safe—nano-based gene delivery with low toxicity
Fast—quick fifteen-minute protocol makes PureFection ideal for high-throughput transfections
PEG-it Concentration Reagent
Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
Cost-effective—contributes to more efficient transductions
Powerful—concentrate lentiviral particles 10- to 100-fold
Scalable—enables isolation of virus from large volumes of media
Safe—supports a range of applications
Found to be non-toxic to all target cell lines tested, including ES cells
Prevent toxic and immunogenic reactions by avoiding accumulation of cellular debris
TransDux™ MAX Transduction Reagent
Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
Non-toxic—no need to change out media after infection
Versatile—works with all types of packaged lentivirus
Flexible—enhances transduction efficiency in a wide variety of cell lines
Broadly compatible—does not interfere with downstream gene expression or functional assays
*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1).
Related Products
LentiSuite Deluxe Kit (includes HEK293TN Producer Cells, pPACKH1, PureFection, PEG-it, TransDux MAX, & Global UltraRapid Titering Kit) 10 preps
System Biosciences
LentiStarter 3.0 Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 2-5 preps
System Biosciences
TransDux MAX Lentivirus Transduction Enhancer
System Biosciences
pPACKH1 HIV Lentivector Packaging Kit
System Biosciences
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
System Biosciences
pPACKH1-XL HIV Lentivector Packaging Kit
System Biosciences
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
System Biosciences
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
System Biosciences
pPACKH1- REV | PVT11093
Nova Lifetech