LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 10 preps

For fast-moving labs generating lentivirus at large scales

Kit Contents

pPACK Packaging System

Reliable—generate high titers of transduction-ready pseudoviral particles
Powerful—efficiently deliver and stably express your lentivector constructs in a wide range of mammalian cells
Well-designed—quantify pseudoviral titers and transduction efficiency using a convenient copGFP control
Flexible—choose from FIV and HIV-based lentivector packaging kits
PureFection Transfection Reagent

Flexible—highly effective transfection technology supports a range of applications
Compatible with most cell types
Works with both plasmid DNA and siRNAs
Cost-effective—excellent alternative to expensive, lipid-based products
Safe—nano-based gene delivery with low toxicity
Fast—quick fifteen-minute protocol makes PureFection ideal for high-throughput transfections
PEG-it Concentration Reagent

Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
Cost-effective—contributes to more efficient transductions
Powerful—concentrate lentiviral particles 10- to 100-fold
Scalable—enables isolation of virus from large volumes of media
Safe—supports a range of applications
Found to be non-toxic to all target cell lines tested, including ES cells
Prevent toxic and immunogenic reactions by avoiding accumulation of cellular debris
TransDux™ MAX Transduction Reagent

Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
Non-toxic—no need to change out media after infection
Versatile—works with all types of packaged lentivirus
Flexible—enhances transduction efficiency in a wide variety of cell lines
Broadly compatible—does not interfere with downstream gene expression or functional assays
*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1).