Lentiviral Dual Reporter: UBC-RFP-T2A-Luciferase pre-packaged virus

Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression.

SBI's lentivector-based imaging systems offer safe, stable, and effective gene delivery in virtually all mammalian cells in tissue culture and in whole animal models.

The UBC-RFP-T2A-Luciferase Lentivector for In Vivo Imaging leverages SBI’s well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. Because the vector becomes integrated into the genome, you can use this vector to create stable reporter cell lines.

The vector co-expresses RFP and luciferase from a UbC promoter for low expression in most cell types including primary cells and stem cells. Co-expression is mediated by a T2A element. Available as either a plasmid or pre-packaged virus.

With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs:

• You’re working with difficult-to-transfect cells
• You’d like to create stable reporter cell lines
• You’d like to integrate your reporter into the genome

• You’d like episomal expression
• You’d like to avoid introducing foreign sequences

Figure 1. Examples of how SBI’s BLIV lentivectors deliver strong luciferase activity after both transduction and transfection.

Figure 2. Examples of how SBI’s BLIV minicircle vectors deliver strong luciferase activity in small animal models.

Figure 3. Examples of how SBI’s BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity.