- Home
- Bioluminescent Imaging
- Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug)
System Biosciences
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug)
- SKU:
- BLIV507MN-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
- Qualifications:
- Academic/ Non-profit customers only
Description
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug). Cat# BLIV507MN. Supplier: SBI System Biosciences
Overview
Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression.
SBI's minicricle-based imaging systems offer safe and efficient gene delivery with a number of benefits:
- Foreign DNA-free—they are devoid of bacterial plasmid sequences, enabling gene therapy development and other in vivo applications
- More easily transfected—their small molecular size increases transfection efficiency
- Non-integrating—they reduce the risk of insertional mutagenesis due to their episomal nature
- Long-lasting—they deliver sustained expression over a period of weeks or months
- Safe—they are less toxic to cells, inducing lower stress and inflammation responses than other gene delivery systems
The MSCV-GFP-T2A-Luciferase Minicircle for In Vivo Imaging leverages SBI’s easy-to-use Minicircle Technology to deliver the bright copGFP and luciferase to target cells. The reporters are co-expressed from an MSCV promoter for high expression in hematopoietic and stem cells, with co-expression mediated by a T2A element. The MSCV-GFP-T2A-Luciferase Minicircle is available as both parental plasmid and ready-to-transfect minicircle DNA*.
Which BLIV construct should you choose?
With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs:
Choose a lentivector when:
- You’re working with difficult-to-transfect cells
- You’d like to create stable reporter cell lines
- You’d like to integrate your reporter into the genome
Choose a minicircle vector* when you’re working with transfectable cells and:
- You’d like episomal expression
- You’d like to avoid introducing foreign sequences
Choose your promoter using the following table:
*Note that Minicircle Parental Vectors are only available to academic/non-profit customers. Researchers at commercial organization may only purchase the pre-made minicircle BLIVs.
Supporting Data
See SBI’s BLIV Vectors in action
Figure 1. Examples of how SBI’s BLIV lentivectors deliver strong luciferase activity after both transduction and transfection.
Figure 2. Examples of how SBI’s BLIV minicircle vectors deliver strong luciferase activity in small animal models.
Figure 3. Examples of how SBI’s BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity.
Related Products


Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase Parental Plasmid (10 ug)
System Biosciences


Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug)
System Biosciences


Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase Parental Plasmid (10 ug)
System Biosciences
Customers Also Viewed


Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug)
System Biosciences

Lysosome Labeling Lentivirus (LAMP1)(GFP) | LVP718
ABMGood


XStamp BHP1 exosome brain homing peptide targeting lentivector
System Biosciences

miRZip-877 anti-miR-877 microRNA construct
System Biosciences

miRZip-605 anti-miR-605 microRNA construct
System Biosciences

miRZip-590-5p anti-miR-590-5p microRNA construct
System Biosciences

miRZip-511 anti-miR-511 microRNA construct
System Biosciences

miRZip-489 anti-miR-489 microRNA construct
System Biosciences

miRZip-454 anti-miR-454 microRNA construct
System Biosciences

miRZip-425 anti-miR-425 microRNA construct
System Biosciences

Human pre-microRNA Expression Construct Lenti-miR-452
System Biosciences


Exo-Flow 2.0 CD63 Analysis Kit (for Serum or Plasma)
System Biosciences

TRAF7-R655Q Plasmid | PVT18202
Nova Lifetech

SURE Escherichia coli Strains | S0041
Nova Lifetech

sgRNA vector Plasmid | PVT12071
Nova Lifetech

Rosetta-gami (DE3) pLysS Escherichia coli Strains | S0022
Nova Lifetech

M15 (pREP4) Strains | STR0027
Nova Lifetech

C58 Agrobacterium Strain | STR3108
Nova Lifetech

miRZip-99a anti-miR-99a microRNA construct
System Biosciences

miRZip-9 anti-miR-9 microRNA construct
System Biosciences

miRZip-888 anti-miR-888 microRNA construct
System Biosciences

miRZip-638 anti-miR-638 microRNA construct
System Biosciences


Exo-Flow32 CD81 IP kit
System Biosciences


Exo-Fect Exosome Transfection Kit
System Biosciences

BJ5183/ pAdEasy-1 Escherichia coli Strains | S0125
Nova Lifetech

Autophagosome Labeling Lentivirus (MAP1LC3B)(CFP) | LVP732
ABMGood

Instant Lentivirus Titration Card | G615
ABMGood

Lenti-III-Blank Lentivirus | LVP687
ABMGood


SARS-CoV-2 Spike Glycoprotein Expression (S) Lentivector, pCDH-CMV-SARS-CoV-2-S-EF1α-Puro (Plasmid)
System Biosciences
![rno-miRNome MicroRNA Profiling Kit [Rat] rno-miRNome MicroRNA Profiling Kit [Rat]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14572/14840/sbi%20system%20biosciences_1633075021__00205.original__20411.1633076864.jpg?c=1)
rno-miRNome MicroRNA Profiling Kit [Rat]
System Biosciences


pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
System Biosciences


PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector
System Biosciences

miRZip-let7b anti-miR-let7b microRNA construct Bacterial streak
System Biosciences

miRZip-let7a anti-miR-let7a microRNA construct
System Biosciences

miRZip-98 anti-miR-98 microRNA construct
System Biosciences

miRZip-96 anti-miR-96 microRNA construct
System Biosciences

miRZip-92-2* anti-miR-92-2* microRNA star construct
System Biosciences

miRZip-885-5p anti-miR-885-5p microRNA construct
System Biosciences

miRZip-720 anti-miR-720 microRNA construct
System Biosciences

miRZip-7 anti-miR-7 microRNA construct
System Biosciences

miRZip-616 anti-miR-616 microRNA construct
System Biosciences

miRZip590-3p anti-miR-590-3p microRNA construct
System Biosciences

miRZip-582-5p anti-miR-582-5p microRNA construct
System Biosciences

miRZip-551a anti-miR-551a microRNA construct
System Biosciences

miRZip-548d-5p anti-miR-548d-5p microRNA construct
System Biosciences

miRZip-548d-3p anti-miR-548d-3p microRNA construct
System Biosciences

miRZip-544 anti-miR-544 microRNA construct
System Biosciences

miRZip-542-3p anti-miR-542-3p microRNA construct
System Biosciences

miRZip-519c-5p anti-miR-519c-5p microRNA construct
System Biosciences

miRZip-519c-3p anti-miR-519c-3p microRNA construct
System Biosciences