System Biosciences
Minicircle Single Reporter: EF1 Luciferase DNA (30 ug)
- SKU:
- BLIV511MC-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 30 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
Minicircle Single Reporter: EF1 Luciferase DNA (30 ug). Cat# BLIV511MC. Supplier: SBI System Biosciences
Overview
Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression.
SBI's minicricle-based imaging systems offer safe and efficient gene delivery with a number of benefits:
- Foreign DNA-free—they are devoid of bacterial plasmid sequences, enabling gene therapy development and other in vivo applications
- More easily transfected—their small molecular size increases transfection efficiency
- Non-integrating—they reduce the risk of insertional mutagenesis due to their episomal nature
- Long-lasting—they deliver sustained expression over a period of weeks or months
- Safe—they are less toxic to cells, inducing lower stress and inflammation responses than other gene delivery systems
The EF1α-Luciferase Minicircle for In Vivo Imaging leverages SBI’s easy-to-use Minicircle Technology to deliver luciferase to target cells. The reporter is expressed from an EF1α promoter for medium expression in most cell types including primary and stem cells. The EF1α-Luciferase Minicircle is available as both parental plasmid and ready-to-transfect minicircle DNA*.
Which BLIV construct should you choose?
With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs:
Choose a lentivector when:
- You’re working with difficult-to-transfect cells
- You’d like to create stable reporter cell lines
- You’d like to integrate your reporter into the genome
Choose a minicircle vector* when you’re working with transfectable cells and:
- You’d like episomal expression
- You’d like to avoid introducing foreign sequences
Choose your promoter using the following table:
*Note that Minicircle Parental Vectors are only available to academic/non-profit customers. Researchers at commercial organization may only purchase the pre-made minicircle BLIVs.
Supporting Data
See SBI’s BLIV Vectors in action
Figure 1. Examples of how SBI’s BLIV lentivectors deliver strong luciferase activity after both transduction and transfection.
Figure 2. Examples of how SBI’s BLIV minicircle vectors deliver strong luciferase activity in small animal models.
Figure 3. Examples of how SBI’s BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity.
Related Products


Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Dual Reporter: EF1-RFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Single Reporter: CMV-Luciferase Minicircle (30 ug)
System Biosciences


Minicircle Single Reporter: EF1 alpha-Luciferase Parental Plasmid (10 ug)
System Biosciences
Customers Also Viewed


XPack MSCV-XP-Luciferase-EF1-Puro Expression Lentivector
System Biosciences


Rat Nestin Differentiation Reporter (pGreenZeo, Virus)
System Biosciences
![pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM virus] pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14477/14745/sbi%20system%20biosciences_1633075021__00205.original__95326.1633076848.jpg?c=1)
![pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM virus] pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14477/30002/LL210PA__85041.1640249168.png?c=1)
pLL-EF1a-RFP-T2A-Puro [Lenti-Labeler™ virus]
System Biosciences
![pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro [Lenti-LabelerTM virus] pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14471/14739/sbi%20system%20biosciences_1633075021__00205.original__18265.1633076848.jpg?c=1)
pLL-EF1a-rFLuc-T2A-GFP-mPGK-Puro [Lenti-Labeler™ virus]
System Biosciences
![pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid] pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14448/14716/sbi%20system%20biosciences_1633075021__00205.original__77613.1633076844.jpg?c=1)
![pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid] pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14448/29979/LL150PA__06286.1640242661.png?c=1)
pLL-CMV-Luciferase-T2A-Puro [Lenti-Labeler™ plasmid]
System Biosciences


PinPoint Donor Positive Control Vector: CM->Luciferase (pPP-CMV-Luc-pA-attB-Puro-pA)
System Biosciences


PinPoint Donor Positive Control Vector: CAG->GFP+Luciferase (pPP-CAG-GFP-T2A-Luc-WPRE-pA-attB-Puro-pA)
System Biosciences

pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged)
System Biosciences


Minicircle Single Reporter: CMV-Luciferase Minicircle (30 ug)
System Biosciences


Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Dual Reporter: EF1-RFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase Parental Plasmid (10 ug)
System Biosciences


Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase DNA (30 ug)
System Biosciences


Lentiviral Triple Reporter: UBC-Luciferase-RFP-TK pre-packaged virus
System Biosciences


Lentiviral Triple Reporter: UBC-Luciferase-RFP-TK plasmid (10 ug)
System Biosciences


Lentiviral Triple Reporter: CMV-Luciferase-RFP-TK plasmid (10 ug)
System Biosciences

pHIV-Luciferase Plasmid | PVT17460
Nova Lifetech

pGL3 BRE Luciferase vector Plasmid | PVT11989
Nova Lifetech

NL4-3 mCherry Luciferase Plasmid | PVT17706
Nova Lifetech

High titer Large, 200 µL (> 1 x 10^8 IFU/mL) | 568-S502HL
101Bio

Ultra-high Titer, 200 µL (> 1 x 10^9 IFU/mL) | 568-S501UL
101Bio

High titer, 200 µL (> 1 x 10^8 IFU/mL) | 568-S501HL
101Bio

iPSC generation | 568-S401
101Bio

EGR1-RFP (Puro) Lentivirus | LVP1120-P
GenTarget

EGR1-RFP (Neo) Lentivirus | LVP1120-N
GenTarget

EGR1-RFP (Bsd) Lentivirus | LVP1120-B
GenTarget

EGR1-GFP (RFP) Lentivirus | LVP1119-R
GenTarget

EGR1-GFP (Neo) Lentivirus | LVP1119-N
GenTarget

ZYG11B Clone BC029832
Cusabio

ZWINT Clone BC020979
Cusabio

ZSWIM1 Clone BC001672
Cusabio

ABCB6 Clone BC000559 pUC
Cusabio

Anti-Mesothelin (SS1) CAR7 Lentivirus | LVP757
ABM Good

Anti-Mesothelin (SS1) CAR6 Lentivirus | LVP756
ABM Good

Anti-Mesothelin (SS1) CAR5 Lentivirus | LVP755
ABM Good

Anti-Mesothelin (SS1) CAR3 Lentivirus | LVP753
ABM Good

Anti-Mesothelin (SS1) CAR2 Lentivirus | LVP752
ABM Good

Anti-Mesothelin (SS1) CAR1 Lentivirus | LVP751
ABM Good

CD Lentiviral Vector | LV678
ABM Good

Lenti-III-CFP-N Partial Kit | LV672
ABM Good

Peroxisome Labeling Lentivirus (PTS)(RFP) | LVP725
ABM Good

Lysosome Labeling Lentivirus (LAMP1)(GFP) | LVP718
ABM Good

Plasma Membrane Labeling Lentivirus (LCK)(CFP) | LVP714
ABM Good

Plasma Membrane Labeling Lentivirus (LCK)(RFP) | LVP713
ABM Good

Plasma Membrane Labeling Lentivirus (LCK)(GFP) | LVP712
ABM Good

Plasma Membrane Labeling Lentivirus (Farnesyl Signal)(RFP) | LVP710
ABM Good

Plasma Membrane Labeling Lentivirus (Farnesyl Signal)(GFP) | LVP709
ABM Good

Nucleus Labeling Lentivirus (LMNB1)(GFP) | LVP706
ABM Good

qPCR Retrovirus Titration(Titer) Kit | G949
ABM Good