pAAVK-EF1α -MCS AAVanced™ Cloning and Expression Vector

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SKU:
AAV502A-1
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Size:
10 ug
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  • pAAVK-EF1α -MCS AAVancedTM Cloning and Expression Vecto
  • pAAVK-EF1α -MCS AAVanced™ Cloning and Expression Vector
€723.00
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Description

pAAVK-EF1α -MCS AAVancedTM Cloning and Expression Vector. Cat# AAV502A. Supplier: SBI System Biosciences

Take advantage of AAV-based gene delivery for gene therapy development and other applications with this single promoter vector, pAAVK-EF1α-MCS.
  • Optimized for high titer rAAV production
  • Available in single and dual promoter formats
  • Compatible with any AAV packaging system
  • Designed for easy & efficient cloning
  • Choose fluorescent or antibiotic selection markers

Overview

Harness AAV technology with SBI’s AAVanced rAAV Vectors

Widely used for gene therapy development and gene editing in vivo because of their broad tropism, lack of associated disease, the ability to transduce both dividing and non-dividing cells, and long-term transgene expression, recombinant AAV vectors are becoming increasingly popular. To help researchers take advantage of the powerful AAV system, SBI has developed a series of AAV vectors optimized for easy cloning and high titers. The pAAVK-EF1α-MCS AAVanced™ Cloning and Expression Vector is a streamlined AAV vector that enables the expression of a gene-of-interest by the EF1α promoter.

Because packaging into the AAV capsid limits the size of AAV vectors, the total amount of DNA between the two ITRs in SBI’s AAVanced Vectors needs to be 5 kb or less. For this reason, the streamlined pAAVK-EF1α-MCS vector is the best choice for researchers who need to insert larger amounts of DNA sequence.

Choose the right AAVanced Vector for your projectSBI’s family of AAVanced Cloning and Expression Vectors support a range of projects:

  • Optimized for high titer rAAV production
  • Available in single and dual promoter formats
  • Compatible with any AAV packaging system
  • Designed for easy & efficient cloning
  • Choose fluorescent or antibiotic selection markers

 

Supporting Data

Effective gene delivery and expression with AAVanced Cloning and Expression Vectors

 

Figure 1. SBI’s single promoter AAVanced Cloning and Expression vectors are delivered effectively to target cells and express the desired markers. Representative images for GFP/RFP/Puro marker expression in HT1080 cells transduced with pAAVK-EF1α-MCS-T2A-EGFP (top row), pAAVK-EF1α-MCS-T2A-mRFP (middle row), or pAAVK-EF1α-MCS-T2A-Puro (bottom row). The top two rows compare cells transduced with the indicated vector packaged into AAV particles versus cells transfected with the same vector. Both conditions show good expression of GFP or RFP. The bottom row shows the number of cells present after three days of puromycin selection in the well with or without infection with pAAVK-EF1-MCS-2A-Puro packaged into AAV particles.

 

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