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System Biosciences

PB-CMV-GreenPuro Scramble Hairpin Control Vector

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SKU:
PBSI505-000PA-1
Availability:
Usually Shipped in 5 Working Days
Size:
10 ug
Shipping Temperature:
Blue Ice/ Dry Ice
Qualifications:
Requires signed license from commercial customers
  • PB-CMV-GreenPuro Scramble Hairpin Control Vector
  • PB-CMV-GreenPuro Scramble Hairpin Control Vector
€737.00

Description

PB-CMV-GreenPuro Scramble Hairpin Control Vector. Cat# PBSI505-000PA. Supplier: SBI System Biosciences

Easily implement a scramble hairpin control for your PiggyBac-delivered shRNA studies with this ready-to-transfect vector
  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers

Overview

Easy and consistent shRNA delivery and expression
Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA . The PB-CMV-GreenPuro Scramble Hairpin Control Vector (Cat.# PBSI505-000PA-1) is a ready-to-transfect control for your PiggyBac shRNA projects. The vector features GFP and puromycin resistance co-expressed from the strong CMV promoter, with co-expression mediated by the T2A element. An H1 promoter drives expression of the scramble hairpin.
 
 
 
With the PiggyBac Transposon System, you can:
  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology. For end user license information, see the following:
  • Academic Customers—End-User License Information
  • Commercial Customers—End-User License Information
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.
 

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

 

 

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal
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