System Biosciences
pCDH-Cuo-MCS-EF1-CymR-T2A-Puro
- SKU:
- QM800A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
pCDH-Cuo-MCS-EF1-CymR-T2A-Puro. Cat# QM800A. Supplier: SBI System Biosciences
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
Overview

- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
How It Works
Tightly-controlled, inducible gene expression
SBI’s SparQ Cumate Switch System delivers robust, titratable gene expression with low background through three components:
- Cumate, a non-toxic, small-molecule inducer
- CymR, a repressor that binds to cumate operator sequences in the absence of cumate
- SparQ Lentivector that contains an MCS to clone-in your gene-of-interest, the cumate inducible promoter with cumate operator sequences (CuO) upstream of the MCS, and one or more markers
CymR has a high binding affinity for cumate and, as more cumate is added, fewer CymR molecules bind to the CuO sequences in the promoter resulting in increased expression. Exhibiting much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.
Supporting Data
Tight expression control with low background with the SparQ Cumate Switch System
Figure 1. Get lower background and higher induction with the SparQ Cumate Switch System than other inducible systems.
Figure 2. Gene expression with the SparQ Cumate SwitchSystem can be turned on and off, then on again.
Figure 3. Gene expression with the SparQ Cumate Switch System is titratable, with increasing amounts of cumate inducing a linear increase in gene expression.
Figure 4. With the SparQ System, gene expression can also be titrated by increasing the amount of transduced SparQ lentivirus, even up to 30 MOI.
Related Products

pCDH- EF1- MCS- T2A- Puro Plasmid | PVT2335
Nova Lifetech


pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector
System Biosciences


pCDH-EF1-CymR-T2A-Puro (plasmid)
System Biosciences


pCDH-EF1-CymR-T2A-Puro (virus)
System Biosciences


pCDH-EF1-MCS*-T2A-GFP
System Biosciences
Customers Also Viewed


pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector
System Biosciences


pCDH-CMV-MCS cDNA Cloning and Expression Vector
System Biosciences

EGR1-GFP (Bsd) Lentivirus | LVP1119-B
GenTarget

ZZZ3 Clone BC035818
Cusabio

ZZZ3 Clone BC035079
Cusabio

ZYX Clone BC008743
Cusabio

Lenti-SV40Tt Lentivirus | G258
ABMGood


Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)
System Biosciences


pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector
System Biosciences


pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug
System Biosciences


pCDH-EF1-MCS*-T2A-GFP
System Biosciences


pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector
System Biosciences


pCDH-EF1-MCS cDNA Cloning and Expression Vector
System Biosciences


pCDH-EF1-CymR-T2A-RFP (virus)
System Biosciences


pCDH-EF1-CymR-T2A-RFP (plasmid)
System Biosciences


pCDH-EF1-CymR-T2A-Puro (virus)
System Biosciences


pCDH-EF1-CymR-T2A-Puro (plasmid)
System Biosciences


pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector
System Biosciences


pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector
System Biosciences


pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector
System Biosciences


pCDH-CuO-MCS-EF1-RFP dual promoter lentivector
System Biosciences


pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector
System Biosciences


pCDH-CuO-MCS-EF1-GFP dual promoter lentivector
System Biosciences


pCDH-CuO-MCS empty cloning lentivector
System Biosciences


pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector
System Biosciences
![pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged] pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14118/14386/sbi%20system%20biosciences_1633075021__00205.original__73665.1633076788.jpg?c=1)
![pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged] pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14118/30041/CD510VB__88999.1640265183.png?c=1)
pCDH-CMV-MCS-EF1-aRFP cDNA Clon/Exp Vector [pre-packaged]
System Biosciences

Mouse pre-microRNA Expression Construct mir-9-3
System Biosciences

Mouse pre-microRNA Expression Construct mir-9-2
System Biosciences

Mouse pre-microRNA Expression Construct mir-293+294+295
System Biosciences

miRZip-9 anti-miR-9 microRNA construct
System Biosciences

Human pre-microRNA Expression Construct Lenti-miR-9-2
System Biosciences

Human pre-microRNA Expression Construct Lenti-miR-9-1
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


Exosome-Depleted FBS Media Supplement
System Biosciences


Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media
System Biosciences


Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
System Biosciences


CLOuD9 Inducer Reagent (0.5M) - 400 ul
System Biosciences
![Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12523/12787/sbi%20system%20biosciences_1633075021__00205.original__98344.1633076540.jpg?c=1)
![Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12523/15418/Screenshot_2021-12-05_145530__76330.1638698497.png?c=1)
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
System Biosciences


Cas9 RT-PCR Primer Set (50ul at 5uM for each primer set)
System Biosciences


Basic Exo-Flow kit without any coupled antibodies (Uncoupled magnetic streptavidin beads, Wash and Elution Buffers, Exo-FITC stain)
System Biosciences


Anti-CD9 Antibody (rabbit anti-human) with goat anti-rabbit HRP secondary antibody
System Biosciences


ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Pre-packaged Lentivirus)
System Biosciences


ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid)
System Biosciences


293TN Producer Cell Line
System Biosciences


"S" SARS-CoV-2 Spike Glycoprotein mRNA (10 ug, Transfection-ready)
System Biosciences