System Biosciences
pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector
- SKU:
- QM522A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
pCDH-CuO-MCS-T2A-RFP co-inducible T2A lentivector. Cat# QM522A. Supplier: SBI System Biosciences
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
Overview

- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
How It Works
Tightly-controlled, inducible gene expression
SBI’s SparQ Cumate Switch System delivers robust, titratable gene expression with low background through three components:
- Cumate, a non-toxic, small-molecule inducer
- CymR, a repressor that binds to cumate operator sequences in the absence of cumate
- SparQ Lentivector that contains an MCS to clone-in your gene-of-interest, the cumate inducible promoter with cumate operator sequences (CuO) upstream of the MCS, and one or more markers
CymR has a high binding affinity for cumate and, as more cumate is added, fewer CymR molecules bind to the CuO sequences in the promoter resulting in increased expression. Exhibiting much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.
Supporting Data
Tight expression control with low background with the SparQ Cumate Switch System
Figure 1. Get lower background and higher induction with the SparQ Cumate Switch System than other inducible systems.
Figure 2. Gene expression with the SparQ Cumate SwitchSystem can be turned on and off, then on again.
Figure 3. Gene expression with the SparQ Cumate Switch System is titratable, with increasing amounts of cumate inducing a linear increase in gene expression.
Figure 4. With the SparQ System, gene expression can also be titrated by increasing the amount of transduced SparQ lentivirus, even up to 30 MOI.
Related Products


pCDH-Cuo-MCS-EF1-CymR-T2A-Puro
System Biosciences


pCDH-CuO-MCS-IRES-GFP co-inducible IRES lentivector
System Biosciences


pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector
System Biosciences


pCDH-CuO-MCS-IRES-RFP co-inducible IRES lentivector
System Biosciences


pCDH-CuO-MCS-T2A-GFP co-inducible T2A lentivector
System Biosciences
Customers Also Viewed

Lenti-SV40Tt Lentivirus | G258
ABMGood


Scramble control hairpin in pCDH-CMV-MCS-EF1-copGFP (CD511B-1)
System Biosciences


pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector
System Biosciences


pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector
System Biosciences


pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector
System Biosciences


pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug
System Biosciences


pCDH-CuO-MCS-IRES-GFP-EF1-CymR-T2A-Puro All-in-one inducible IRES lentivector
System Biosciences


pCDH-CuO-MCS-EF1-RFP+Puro dual promoter and dual marker lentivector
System Biosciences


pCDH-CuO-MCS-EF1-RFP dual promoter lentivector
System Biosciences


pCDH-CuO-MCS-EF1-GFP+Puro dual promoter and dual marker lentivector
System Biosciences


pCDH-CuO-MCS-EF1-GFP dual promoter lentivector
System Biosciences


pCDH-Cuo-MCS-EF1-CymR-T2A-Puro
System Biosciences


pCDH-CuO-MCS empty cloning lentivector
System Biosciences


pCDH-CMV-MCS cDNA Cloning and Expression Vector
System Biosciences

Mouse pre-microRNA Expression Construct mir-293+294+295
System Biosciences


ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Pre-packaged Lentivirus)
System Biosciences


293TN Producer Cell Line
System Biosciences


"S" SARS-CoV-2 Spike Glycoprotein mRNA (10 ug, Transfection-ready)
System Biosciences


"N" SARS-CoV-2 Nucleocapsid mRNA (10 ug, Transfection-ready)
System Biosciences

pRSETB- Clover | PVT10632
Nova Lifetech

plenti-CMV-PCDHAC2 Plasmid | PVTBAV59607
Nova Lifetech

pCR-BluntII-TOPO-SNAI3 Plasmid | PVT17972
Nova Lifetech

pCDH- CMV- IDH1 | PVT10169
Nova Lifetech