System Biosciences

pCT-Apoptosis-Luc (pCMV, Caspase 3/7 activation, cyclic luciferase Tag)

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SKU:: CYTO114-PA
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Description

Description

pCT-Apoptosis-Luc (pCMV, Caspase 3/7 activation, cyclic luciferase Tag). Cat# CYTO114-PA. Supplier: SBI System Biosciences

Study apoptosis with this Cyto-Tracer that exhibits increased luciferase activity with increasing caspase 3/7 activity

Stable lentivector-based system
Great for creating stable reporter cell lines
Ideal for co-localization studies
Monitor cellular dynamics and functional studies in real time
Monitor apoptosis in a range of cell types

Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Apoptosis Cyto-Tracer, pCT-Apoptosis-Luc (CMV), uses cyclic luciferase as a marker for apoptosis. The cyclic luciferase is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.

Stable lentivector-based system
Great for creating stable reporter cell lines
Ideal for co-localization studies
Monitor cellular dynamics and functional studies in real time
Monitor apoptosis in a range of cell types

Choose the Cyto-Tracer that’s right for you

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Apoptosis Cyto-Tracer uses an inactive cyclic luciferase to monitor apoptosis via caspase 3/7 activation. The N- and C-termini of luciferase are covalently joined, thus inactivating the enzyme, via a four-amino acid peptide that is a substrate for caspase 3. Active caspase 3 digests the substrate sequence in the cyclic luciferase, separating the termini and restoring activity. Thus, with the cyclic luciferase reporter, luciferase activity increases as caspase 3 activity increases.