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System Biosciences

pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag)

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CYTO120R-PA
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  • pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag)
  • Exosome Cyto-Tracer, pCT-CD63-RFP
€912.00 - €1,060.00

Description

pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag). Cat# CYTO120R-PA. Supplier: SBI System Biosciences

Label exosomes with RFP by expressing the pCT-CD63-RFP Cyto-Tracer in the parent cells. Great for tracking exosomes and monitoring cargo delivery.
  • Stable lentivector-based system
  • Ideal for co-localization studies
  • Monitor exosome dynamics and functional studies in real time
  • Label exosomes from primary cells, tumor cells, stem cells, and more

Overview

Easily track exosomes with a CD63-RFP fusion
Monitor exosome dynamics with the pCT-CD63-RFP (pCMV) Exosome Cyto-Tracer (also available as a CD63-GFP fusion, pCT-CD63-GFP). Provided as either lentivector or pre-packaged virus, this Cyto-Tracer fuses RFP to the tetraspanin CD63, which then gets packaged into exosomes produced by the transfected cells. Great for creating stable cell lines that produce RFP-labeled exosomes as well as labeling exosomes from primary cells, tumor cells, stem cells, and more.
  • Stable lentivector-based system
  • Ideal for co-localization studies
  • Monitor exosome dynamics and functional studies in real time
  • Label exosomes from primary cells, tumor cells, stem cells, and more
Choose the exosome quantitation method that’s best for your studies

Supporting Data

See some of our exosome Cyto-Tracers in action

The following figure and videos are from:

Garcia NA, et al. Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells. PLoS ONE. 2015. 10(9). PMCID: PMC4578916.

Figure 4 from Garcia, et al. Exosome transfer from CMs to ECs. (A) H9c2 transfected with pCT-CD63-GFP. FACS analysis of 90% GFP positive cells. (B) Representative images from confocal time-lapse microscopy of mouse ECs (ACTB-DsRed) (EC; red) co-cultured with H9C2-CD63-GFP cells (green), previously cultured for 24 h in +/-St medium. Exosome transfer from H9C2 CMs to EC can be observed (S1 and S2 Movies, below). White arrows show CD63-GFP structures inside ECs (C) Representative immunostaining of H9C2-CD63-GFP and HUVEC co-cultures; anti-GFP (green) and anti-CD31 (red). The images illustrate GFP fluorescence from CD63-GFP exosomes in CD31-positive cells (red) after 24 h incubation in +/-St medium. White arrows show CD63-GFP structures inside ECs.

Video S1 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under +St conditions.

 

Video S2 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under -St conditions.

Labeled exosomes. (Top panels) CD63-GFP Cyto-Tracers transfected into a human fibrosarcoma cell line. (Bottom panels) CD9-GFP and CD9-RFP Cyto-tracers co-transfected into HEK293 cells.

 

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