System Biosciences
pGF-SMAD2/3/4-mCMV-EF1-Puro (plasmid)
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- SKU:
- TR203pa-p
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
pGF-SMAD2/3/4-mCMV-EF1-Puro (plasmid). Cat# TR203pa-p. Supplier: SBI System Biosciences
Study oncogenic signaling by co-expressing dscGFP and luciferase in response to SMAD2/3/4 activity at SMAD2/3/4 transcriptional response elements
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
Products
Overview
Monitor oncogenic signaling in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line.
The pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from SMAD2/3/4 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of SMAD2/3/4 TREs, drives expression of dscGFP and luciferase in response to SMAD2/3/4 activity. The result is the ability to quantitatively measure SMAD2/3/4 activity at SMAD2/3/4 TREs by fluorescence and luciferase activity.
- Sort responsive cells with dscGFP
- Measure activity with luciferase
- Leverage SBI’s highly-regarded lentivectors
- Create stable signaling pathway reporter cell lines
- Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines
The pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-SMAD2/3/4-mCMV-EF1α-Puro Lentivector transduced into 293 cells.
*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.Supporting Data
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation
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