pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro)
- Usually Shipped in 5 Working Days
- 10 µ
- Shipping Temperature:
- Blue Ice/ Dry Ice
pGreenFire 2.0-CMV positive control plasmid (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro). Cat# TR410PA-P. Supplier: SBI System Biosciences
Use the pGreenFire 2.0 CMV Positive Control Lentivector as a ready-to-go positive control for any pGreenFire 2.0 project (choose lentivector or packaged virus)
No need to make a positive control for your pGreenFire 2.0 projects—SBI’s already built one for you. With the pGreenFire 2.0 CMV Positive Control Lentivector (pGF2-CMV-rFluc-T2A-GFP-mPGK-Puro), the GreenFire 2.0 cassette is driven by the CMV promoter for strong expression in common cell lines such as HeLa, HEK293, and HT1080. As with all of our pGreenFire 2.0 lentivectors, the GreenFire cassette now consists of red firefly luciferase (rFLuc), a T2A co-expression element, and GFP. The switch to rFLuc opens up the possibility of performing a dual-spectral luciferase assay and also delivers greater sensitivity for in vivo applications than conventional luciferase.
The pGreenFire 2.0 CMV Positive Control Lentivector uses the same smart design as our pGreenFire 2.0 reporters, which adds in a constitutive selection cassette for stable cell line generation while minimizing interference with the upstream GreenFire cassette. By using a weak/moderate mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and carefully arranging the reporter genes, the selection marker is reliably expressed along with rFLuc and GFP.