pGreenFire1-LXRE in SREBP1c (HepG2 cell line)

Monitor cholesterol homeostasis signal transduction in real time With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line.

 

The pGreenFire1-LXRE-in-SREBP1c Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from the Liver X Receptor (LXR) response elements (LXREs) and neighboring regions in the SREBP1c promoter paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of the LXRE/SREBP1c transcriptional elements, drives expression of dscGFP and luciferase in response to LXR activity. The result is the ability to quantitatively measure LXR activity at the SREBP1c promoter by fluorescence and luciferase activity.

• Sort responsive cells with dscGFP
• Measure activity with luciferase
• Leverage SBI’s highly-regarded lentivectors
• Create stable signaling pathway reporter cell lines
• Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines