pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)
- Usually Shipped in 5 Working Days
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control). Cat# TR010PA. Supplier: SBI System Biosciences
Run your pGreenFire projects with confidence with the addition of this negative control that drives copGFP and luciferase with a minimal CMV promoter
Supporting your studies with ready-to-go controls No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with dscGFP (destabilized copGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element.
The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of dscGFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.
The pGreenFire1-mCMV vector is also available with constitutively expressed markers to simplify cell line construction—EF1α-neo (Cat.# TR010PA-N) and EF1α-puro (Cat.# TR010PA-P). All versions of this lentivector are available as lentivector or pre-packaged virus.
See our transcriptional response element reporters in action
Monitor oncogenic pathway reporters
Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis
General pGreenFire data examples
Monitoring NF-κB transactivation