System Biosciences

pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)

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SKU:
TR010PA-1
Availability:
Usually Shipped in 5 Working Days
Size:
10 ug
Shipping Temperature:
Blue Ice/ Dry Ice
  • pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)
  • pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)
€675.00

Description

pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control). Cat# TR010PA. Supplier: SBI System Biosciences

Run your pGreenFire projects with confidence with the addition of this negative control that drives copGFP and luciferase with a minimal CMV promoter

Products

Overview

Supporting your studies with ready-to-go controls No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with dscGFP (destabilized copGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element.
 
The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of dscGFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.
The pGreenFire1-mCMV vector is also available with constitutively expressed markers to simplify cell line construction—EF1α-neo (Cat.# TR010PA-N) and EF1α-puro (Cat.# TR010PA-P). All versions of this lentivector are available as lentivector or pre-packaged virus.

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examples

Monitoring NF-κB transactivation

 

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