piggyBac qPCR copy number kit, 20 reactions 

When you need to know how many PiggyBac integration events have happened, turn to SBI’s PiggyBac qPCR Copy Number Kit. This kit uses qPCR to measure the number of PiggyBac inserts relative to a specific genomic locus—the UCR1 element—with the PiggyBac insert copy number calculated using the cycle threshold (Ct) values of the UCR1 signal relative to the PiggyBac insert signal.

The PiggyBac qPCR Copy Number Kit comes with enough reagents—UCR1 primers, PiggyBac primers, and cell lysis buffer—for twenty copy number determinations, and is compatible with all of SBI’s PiggyBac Vectors.

NOTE: Your cells must be passaged at least once before performing this copy number measurement to ensure that residual, non-integrated piggyBac transposon plasmid does not interfere with the qPCR reaction.