System Biosciences
pLL-CMV-Luciferase-T2A-Puro [Lenti-Labeler™ plasmid]
- SKU:
- LL150PA-1
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
Description
pLL-CMV-Luciferase-T2A-Puro [Lenti-LabelerTM plasmid]. Cat# LL150PA. Supplier: SBI System Biosciences
Reliably label cells with luciferase driven by a strong CMV promoter (and with puro selection) - great for tracking cells, HTS assays, and more
- Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
- Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
- Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
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Overview
Reliable cell labeling, delivered SBI’s family of Lenti-Labeler™ constructs facilitate a wide range of studies—including cell tracking, high-throughput assays, and more—by enabling efficient and reliable labeling of your cells. The pLL-CMV-Luciferase-T2A-Puro Lenti-Labeler™ construct expresses luciferase from the CMV promoter, which delivers strong expression in most commonly-used cell lines (HeLa, HEK293, HT-1080, etc.), and co-expresses the puromycin resistance gene for selection in vitro prior to in vivo use. Available as either fully propagatable, sequence-verified plasmid DNA (Cat.# LL150PA-1) or ready-to-transduce pre-packaged lentivirus (Cat.# LL150VA-1), SBI’s pLL-CMV-Luciferase-T2A-Puro Lenti-Labeler™ is designed for reliability, so you can get to valuable insights faster.
![](https://cdn11.bigcommerce.com/s-i3n9sxgjum/product_images/uploaded_images/moved-359685ecfeb950b162667fe5604c7fe0.png)
- Proven—leverages SBI’s third generation lentivector technology for optimal virus titers
- Validated—sequence-verified from LTR to LTR and expression-verified in HT-1080 cells
- Flexible—available in a range of fluorescent markers, selection markers, and promoters (Table 1)
SBI offers Lenti-Labeler constructs with a range of selection markers, reporter genes, and two different promoters.
Table 1. Available Lenti-Labeler Constructs![](https://cdn11.bigcommerce.com/s-i3n9sxgjum/product_images/uploaded_images/moved-b244a87e45575ecd2f2ffae8e75ea8a7.png)
Supporting Data
SBI’s Lenti-Labelers get your cells labeled efficiently, reliably
Figure 1. SBI Lenti-Labeler constructs reliably and efficiently label cells. Comparison of the number of fluorescently-labeled cells to the total number of cells seen in the corresponding phase contrast images reveals the high labeling efficiency of SBI’s Lenti-Labeler constructs.