pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging

Safely study SARS-CoV-2 interactions 

Designed to efficiently package most third-generation lentivectors, the pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging Mix speeds and simplifies the preparation of lentiviral particles pseudotyped with the SARS-CoV-2 Spike glycoprotein. Based on SBI's highly cited pPACKH1 packaging system but with a truncated SARS-CoV-2 Spike protein¹ replacing the standard VSV-G envelope protein, the pPACK-SPIKE Packaging Mix consists of three plasmids that produce all of the structural and  replication proteins needed to transcribe and package an RNA copy of an expression lentivector into recombinant, “Spike” pseudotyped lentiviral particles. As a result, you can conduct a range of SARS-CoV-2 studies under BSL-2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.

For added convenience we also offer pPACK-BALD™, an envelope protein-free lentivector packaging mix that can be used as a negative control for pPACK-SPIKE studies, or for creating lentivirus particles pseudotyped with the envelope protein of your choice. In addition, you can compare this Spike protein from the original Wuhan-Hu-1 strain to the more prevalent (as of summer 2020) D614G Spike protein with pPACK-SPIKE D614G.

• Based on SBI’s popular and highly cited pPACKH1 Packaging System
• Uses codon-optimized SARS-CoV-2 “S” protein (the original Wuhan-Hu-1 strain) in place of VSV-G envelope protein
• Efficiently incorporates the Spike protein into pseudoviral particles due to a 19 aa C-terminal truncation [1]
• Ideal for COVID-19 research, such as neutralization assays, under BSL2 conditions
• Package any 3rd-generation lentivector reporter of your choice, including SBI’s popular LentiLabeler reporters
• Available as a packaging mix or bundled into a convenient kit that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution
• Use with pPACK-BALD™, an envelope protein-free lentivector packaging system that is an ideal negative control
• Compare to the widespread D614G Spike protein by using pPACK-SPIKE D614G
• Start discovery and development with the mouse-adapted N501Y Spike protein by using pPACK-SPIKE N501Y

pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging Mix is available as stand-alone packaging mixes with sufficient plasmids for 10 reactions (standard size) or 25 reactions (XL), as well as in a convenient kit format that includes PureFection™ Transfection Reagent and PEG-it Virus Precipitation Solution.

See all the available pPACK-SPIKE S protein variants: