Primers
ABI Dye Primers | |||
---|---|---|---|
M13 reverse | 5'-CAGGAAACAGCTATGACC-3' | a.k.a.: M13 Reverse Dye Primer | |
M13(-21) | 5'-TGTAAAACGACGGCCAGT-3' | a.k.a.: -21M13 Dye Primer; -21M13 universal primer | |
sp6 | 5'-ATTTAGGTGACACTATAG-3' | a.k.a.: SP6; SP6 Dye Primer | |
t3 | 5'-ATTAACCCTCACTAAAGGGA-3' | a.k.a.: T3; T3 Dye Primer | |
t7 | 5'-TAATACGACTCACTATAGGG-3' | a.k.a.: T7; T7 Dye Primer | |
Amersham DYEnamic ET primers | |||
-21 M13 forward | 5'-S+SSSSSTGTAAAACGACGGCCAGT-3' | ||
-28 M13 reverse | 5'-AGGAAACAGCTATGACCAT-3' | ||
-28 M13 reverse 2 | 5'-AGGAAACAGCTATGACATG-3' | ||
-29 M13 reverse | 5'-SSSSSST^CAGGAAACAGCTATGACC-3' | ||
-40 M13 forward | 5'-GTTTTCCCAGTCACGACG-3' | ||
PolyT(N) | 5'-TTTTTTTTTTTTTTTTTTTTTTT(a,c,g)-3' | ||
sp6 | 5'-ATTTAGGTGACACTATAG-3' | a.k.a.: SP6; SP6 Dye Primer | |
t3 | 5'-ATTAACCCTCACTAAAGGGA-3' | a.k.a.: T3; T3 Dye Primer | |
t7 | 5'-TAATACGACTCACTATAGGG-3' | a.k.a.: T7; T7 Dye Primer | |
Custom primers from Invitrogen | |||
-100RP | 5'-ATGCAGCTGGCACGACAGGT-3' | ||
-100UP | 5'-CGCTATTACGCCAGCTGGCGA-3' | ||
-21UP | 5'-TGTAAACGACGGCCACT-3' | ||
-40RP | 5'-GGATAACAATTTCACACAGG-3' | ||
pUC/M13 Forward(-40) | 5'-GTTTTCCCAGTCACGAC-3' | a.k.a.: -40UP | |
Promega Dye Primers | |||
M13(-21) | 5'-TGTAAAACGACGGCCAGT-3' | a.k.a.: -21M13 Dye Primer; -21M13 universal primer | |
pUC/M13 Forward(-40) | 5'-GTTTTCCCAGTCACGAC-3' | a.k.a.: -40UP | |
pUC/M13 reverse primer | 5'-CAGGAAACAGCTATGAC-3' | ||
Sugano mouse custom primers | |||
3' end sequencing primer | 5'-CGACCTGCAGCTCGAGCACA-3' | ||
5' end sequencing primer | 5'-CTTCTGCTCTAAAAGCTGCG-3' | ||
Track2 | |||
AG189 | (rev) 5'-CTTTATTTGTGAAATTTGTGATGC-3' | ||
AG195 | (forward): 5'-CATACGATTTAGGTGACACTATAG-3' | ||
custom | |||
custom t7 | |||
M13 -20 | GTAAAACGACGGCCAGT | ||
M13 reverse | 5'-CAGGAAACAGCTATGACC-3' | a.k.a.: M13 Reverse Dye Primer | |
M13(-21) | 5'-TGTAAAACGACGGCCAGT-3' | a.k.a.: -21M13 Dye Primer; -21M13 universal primer | |
M13F | 5-GTAAAACGACGGCCAGT-3 | ||
pDONR201-forward | TCGCGTTAACGCTAGCATGGATCTC | ||
pDONR201-reverse | GTAACATCAGAGATTTTGAGACAC | ||
pET Upstream Primer | 5'-ATGCGTCCGGCGTAGA-3' | ||
pSMART-1777F | GATCCTTTGATTTTCTACCGAAG | ||
pSMART-200R | CTTACGTGCCGATCAAGTCA | ||
sp6 | 5'-ATTTAGGTGACACTATAG-3' | a.k.a.: SP6; SP6 Dye Primer | |
t3 | 5'-ATTAACCCTCACTAAAGGGA-3' | a.k.a.: T3; T3 Dye Primer | |
t7 | 5'-TAATACGACTCACTATAGGG-3' | a.k.a.: T7; T7 Dye Primer | |
T7 Rev | 5'-GCTAGTTATTGCTCAGCGG-3' | ||
T7 Terminator Primer | 5'-GCTAGTTATTGCTCAGCGG-3' | ||
University of Iowa pYX custom primers | |||
pYX-3 (3' end) | 5'-ACTCATACTCTTCCTTTTTCAAT-3' | ||
pYX-7 (5' end) | 5'-TTGCTCACATGTTAATACGAC-3' | ||
University of Tokyo Fugu custom primers | |||
U. of Tokyo Fugu custom 5' end sequencing primer | 5'-CGCTCTAGAACTAGTGGATCCA-3' |