Rab5b Exo-Flow capture kit (Magnetic streptavidin beads, Rab5b-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)

Easily purify exosomes using FACS with the Rab5b Exo-Flow Capture Kit

At a glance
Simply (1) couple the Rab5b-biotin antibody to the magnetic streptavidin beads, (2) use the Rab5b-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick^® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

Our Rab5b Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolation Bead flow separation data for exosomes secreted by HEK293 cells and captured using our Rab5b Exo-Flow Capture Kit. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panel), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panel). The degree of flow separation is shown in the right panel.
Human serum exosomes were isolated from 250 µL serum using ExoQuick^® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).