Rat Nestin Differentiation Reporter (pGreenZeo, Plasmid)

With SBI’s line of pGreenZeo, pRedZeo, and pRedTK Differentation Reporter Vectors, you can monitor stem cell differentiation in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built differentiation reporters come as ready-to-package lentivector plasmids or ready-to-transduce pre-packaged lentivirus. The Rat Nestin pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin resistance from the rat nestin promoter/enhancer elements, enabling visualization of neurogenesis using GFP fluorescence and selection for the desired cells using zeomycin.

• Create stable neurogenesis-reporting cell lines
• Monitor multiple lineages simultaneously
• Track differentiation in live cells in real time

Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Neural differentiation reporters

Myogenic differentiation reporters

Hematopoietic differentiation reporters

Structural differentiation reporters

Signaling differentiation reporters

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Rat Nestin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch rat neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonstrating the specificity of SBI’s rat Nestin pGreenFire Differentiation Reporter.

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

Figure 3. Additional differentiation reporter data.