System Biosciences

Retro-Concentin

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SKU:: RV100A-1
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Availability:: Usually Shipped in 5 Working Days
Size:: 100 ml
Shipping Temperature:: RT or Blue Ice

zł1,845.74

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Description

Description

Retro-Concentin. Cat# RV100A. Supplier: SBI System Biosciences

Overview

Increase the infectivity of any retrovirus by concentrating with Retro-Concentin

Quantitatively precipitate any retrovirus with Retro-Concentin™. Instead of using lengthy spins in an ultracentrifuge, Retro-Concentin enables concentration of retroviruses directly from culture medium with a simple one (or an optional two) low-speed centrifugation steps.

In addition, Retro-Concentin stabilizes the retroviruses for frozen storage, providing an additional advantage over other concentration methods.

Each preparation can handle up to 200 mL of retroviral supernatant, which is compatible with most centrifuges, and the resulting pellet can be dissolved in the volume of buffer that meets your experimental requirements.

With Retro-Concentin, you get simple and effective retrovirus concentration:

Fast protocol
Cost-effective
Up to 100-fold concentration of retrovirus particles

How It Works

Retro-Concentin supports a fast, simple, and ultracentrifugation-free workflow

Simply add Retro-Concentin to retrovirus supernatant, incubate at 4°C overnight, and then spin at 1,500g for 30-minutes at 4°C. An optional spin in a microfuge tube at 12,000 rpm for 2 minutes can further concentrate the retrovirus particles.

Supporting Data

Retrovirus particles concentrated with Retro-Concentin are non-toxic and effective at transduction

Figure 1. Retrovirus particles concentrated with Retro-Concentin are non-toxic and effective at transduction. Transduced cells show normal morphology and with no obvious cytotoxicity, and were successfully used for generating induced pluripotent stem cells.

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