System Biosciences
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
- SKU:
- RA806A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 20 preps
- Shipping Temperature:
- Blue Ice
Description
SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns). Cat# RA806A. Supplier: SBI System Biosciences
Overview
Looking for biomarkers? Studying exosomal RNAs (exoRNAs)? Turn to SBI’s SeraMir Exosome RNA Purification Kit for optimized isolation of exoRNAs. The kit includes ExoQuick for efficient exosome isolation from serum, plasma, or ascites fluid, and SeraMir columns and reagents for purification of RNA from your isolated exosomes (for exoRNA isolation from other biofluids, try the SeraMir Exosome RNA Purification Kit for Media and Urine).
Reliable, reproducible exoRNA isolation from serum, plasma, or ascites fluid
With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.
The SeraMir Exosome RNA Purification Kit includes everything you need for optimized isolation of exosomal RNAs from serum, plasma, or ascites fluid samples—ExoQuick for fast and efficient exosome isolation and a phenol-free lysis buffer and rapid spin columns to extract RNA from your isolated exosomes.
Choose the SeraMir Kit that’s right for you

How It Works
Go from sample to amplified exoRNAs in a single day
- Isolate exosomes from patient biofluids with the included ExoQuick reagent
- Purify exoRNAs with SeraMir columns
Purified exoRNAs are fully compatible with most downstream applications, such as qPCR, microarray analysis, or NGS.
Isolate serum exosomes and purify exoRNAs
Tail exoRNAs and synthesize double-tagged cDNA
Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis
Supporting Data
Better qPCR profiling with SeraMir
Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.
Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.
Related Products


ExoQuick Exosome RNA Column Purification kit
System Biosciences

SeraMir Exosome RNA Ampflication Kit
System Biosciences


SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
System Biosciences

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)
System Biosciences


SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
System Biosciences
Customers Also Viewed


SeraMir Exosome RNA Amplification kit from Media and Urine (10ml ExoQuick-TC and 10 exoRNA columns)
System Biosciences


SeraMir Exosome RNA Purification kit (10ml ExoQuick-TC and 10 exoRNA columns) 10 preps
System Biosciences


ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
System Biosciences


ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
System Biosciences


ExoQuick PLUS Exosome Purification Kit - for serum and plasma
System Biosciences


ExoQuick Exosome RNA Column Purification kit
System Biosciences


ExoQuick-LP for lipoprotein pre-clear & exosome isolation
System Biosciences


ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL)
System Biosciences


ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media
System Biosciences


ExoQuick exosome precipitation solution (5 ml)
System Biosciences


ExoQuick TC 50 ml
System Biosciences


ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
System Biosciences


ExoQuick TC 10 ml
System Biosciences


ExoQuick exosome precipitation solution (20 ml)
System Biosciences


ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)
System Biosciences


ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
System Biosciences


Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50ml ExoQuick-TC, RA805A-1 and RA812A-1 components)
System Biosciences


EVeryRNA™ EV RNA Purification System with ExoQuick-TC EV Isolation (10 ml)
System Biosciences


FluoroCet Exosome Quantitation Kit
System Biosciences

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)
System Biosciences


EVeryRNA™ EV RNA Purification System with ExoQuick EV Isolation (5 ml)
System Biosciences


XStamp Pro anti-PD-1 EV Targeting Kit
System Biosciences


SmartSEC™ HT EV Isolation System (for Serum & Plasma)
System Biosciences


Exo-Urine EV Isolation Kit
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


Exosome-Depleted FBS Media Supplement
System Biosciences


Exo-FITC Exosome FACS stain
System Biosciences

pLenti-PGK-Blank Virus | LVP627
ABM Good

AAV Packaging Mix (Serotype 4) & DNAfectin Combo Pack | AAV1004-G2100
ABM Good

AAV Packaging Mix (Serotype 2) | AAV1002
ABM Good

Adeno-8™ pShuttle-2A-RFP Complete Cloning Kit | A800
ABM Good


XStamp Pro Streptavidin Customizable EV Targeting Kit
System Biosciences


XPack MSCV-XP-GFP-EF1-Puro Expression Lentivector
System Biosciences


XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)
System Biosciences

SeraMir Exosome RNA Ampflication Kit
System Biosciences

miRZip-184 anti-miR-184 microRNA construct
System Biosciences


Exosome ELISA Complete Kit (CD9 detection) 96 reactions
System Biosciences


Exosome ELISA Complete Kit (CD63 detection) 96 rxns
System Biosciences


Exosome binding buffer for ELISAs (20 ml)
System Biosciences


Exo-Fect Exosome Transfection Kit
System Biosciences


Cas9 Nickase: CAG-hspCas9-nickase-H1-gRNA SmartNickase vector
System Biosciences

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro Plasmid | PVT17271
Nova Lifetech

Anti-Mesothelin (SS1) CAR4 Lentivirus | LVP754
ABM Good

Autophagosome Labeling Lentivirus (MAP1LC3B)(GFP) | LVP730
ABM Good

Mitochondria Labeling Lentivirus (MTS)(GFP) | LVP721
ABM Good

qPCR Lentivirus Complete Titration Kit (MasterMix-LR) | LV900-LR
ABM Good

qPCR Lentivirus Complete Titration Kit (MasterMix-R) | LV900-R
ABM Good

AAV Packaging Mix (Serotype 6) & DNAfectin Combo Pack | AAV1006-G2100
ABM Good

AAV Packaging Mix (Serotype 4) | AAV1004
ABM Good

AAV Packaging Mix (Serotype 2) & DNAfectin Combo Pack | AAV1002-G2100
ABM Good