System Biosciences
Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns
- SKU:
- PB210PA-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 ug
- Shipping Temperature:
- Blue Ice/ Dry Ice
- Qualifications:
- Requires signed license from commercial customers
Description
Super piggyBac Transposase expression vector (replaces PB200A-1), 50 rxns. Cat# PB210PA. Supplier: SBI System Biosciences
Overview
Get easy, consistent transgenesisAt the heart of SBI’s PiggyBac Transposon System* is the Super PiggyBac Transposase, an engineered, hyperactive version of the enzyme that can efficiently integrate any-sized DNA insert into the genome.Why use the PiggyBac Transposon System?Easy, consistent transgenesis with no limits on cargo size—For transgenesis that’s easy, consistent, and not limited by cargo size, SBI’s PiggyBac Transposon System is an excellent choice. The system consists of a PiggyBac Vector and the Super PiggyBac Transposase which recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA sites. The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors.
Footprint-free removal that leaves no PiggyBac sequences behind—In addition to ease-of-use, consistency, and the lack of limits on DNA insert size, what sets this system apart is the ability to reverse the integration reaction in a footprint-free way—with the Excision Only PiggyBac Transposase (Cat.# PB220PA-1), the ITRs and cargo that the Super PiggyBac Transposase integrates into the genome can be removed, leaving behind the original genomic sequence and nothing else.
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (Cat.# PBC100A-1)
Customer AgreementsAcademic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.For For end user license information, see the following:
- Academic Customers—End-User License Information
- Commercial Customers—End-User License Information
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.
How It Works
The PiggyBac Transposon System’s Cut-and-Paste Mechanism
The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
Step 1 The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
Step 2 The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
Step 3 The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal.
Supporting Data
One transfection can integrate one or more genes that can be precisely removed
Figure 2. Efficient transgenesis with the Super PiggyBac Transposase and both single- and dual-promoter PiggyBac Vectors. (Top four panels) Co-transfection with the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1) and a Dual Promoter PiggyBac Cloning and Expression Vector (Cat.# PB513B-1) into HeLa cells demonstrates the efficient integration delivered by SBI’s PiggyBac Transposon System. After ten days of puromycin selection, only the cells co-transfected with the Super PiggyBac Transposase (+PB, right two panels) show robust growth and GFP fluorescence. (Bottom four panels) Co-transfection with the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1) and a Single Promoter PiggyBac Cloning and Expression Vector (Cat.# PB531A-2) into HEK293 cells further demonstrates the efficient integration delivered by SBI’s PiggyBac Transposon System. After seven days of growth, the majority of cells that received the Super PiggyBac Transposase Expression Vector (+PB, right two panels) were RFP positive.
Figure 3. Simultaneous integration of multiple PiggyBac Vectors is also highly efficient. METHODS: Three different PiggyBac transposon vectors (Cat.# PB513B-1, Cat.# PB533A-2, and Cat.# PB531A-2) were co-transfected with (left panels) or without (right panels) the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1) into Human HT1080 cells. Puromycin and neomycin selection was applied for seven days. The cells that were co-transfected with the Super PiggyBac Transposase Expression Vector were puro and neo resistant, GFP-positive, and RFP-positive. Background GFP-positive cells that are puro resistant stem from random PB513B-1 integrations during the puromycin selection. The non-PiggyBac-mediated integration rate in those cells was extremely low and no RFP-positive cells were identified.
Related Products

Super PiggyBac Transposase (PB200PA- 1) | PVT10877
Nova Lifetech

Super PiggyBac Transposase (PB200PA- 1) plasmid | PVT1602
Nova Lifetech

Tet-on circRNA Expression Vector Plasmid | PVT14643
Nova Lifetech


Excision only piggyBac Transposase expression vector, 50 rxns
System Biosciences

Lenti-III-HA Expression Vector | LV022
ABMGood
Customers Also Viewed


Excision only piggyBac Transposase expression vector, 50 rxns
System Biosciences


pMSCV-Human RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Mouse RORgt-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/14689/sbi%20system%20biosciences_1633075021__00205.original__16600.1633076840.jpg?c=1)
![piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correctio](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14421/29947/PBHR100A-1__86845.1640081714.png?c=1)
piggyBac-HR with GFP+Puro markers and TK selection [MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2] for Gene Correction
System Biosciences


PB-SV40-Neo-EF1-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector
System Biosciences


PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector
System Biosciences


PB-Cuo-miR-302/367-IRES-GFP-EF1-CymR-Puro Inducible iPSC Vector
System Biosciences

Super PiggyBac Transposase (PB200PA- 1) plasmid | PVT1602
Nova Lifetech


pMSCV-Mouse Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


pMSCV-Human Foxp3-EF1-GFP-T2A-Puro piggyBac transposon plasmid
System Biosciences


PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector
System Biosciences


PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector
System Biosciences


PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector
System Biosciences


Cumate Solution, high concentration, 10,000x for use with PiggyBac
System Biosciences

Ultra-high Titer, 200 µL (> 1 x 10^9 IFU/mL) | 568-S501UL
101Bio

iPSC differentiation | 568-S404
101Bio

iPSC gene editing | 568-S403
101Bio

hTERT (CMV, RFP-Bsd) lentivirus in PBS | LVP1130-RB-PBS
GenTarget

H_RE-SEAP (Neo) Lentivirus in PBS | LVP1274-PBS
GenTarget

G_RE-SEAP (Bsd) Lentivirus in PBS | LVP1265-PBS
GenTarget

ER-SEAP (Neo) Lentivirus in PBS | LVP1262-PBS
GenTarget

EF1a Control lentiviral particles (GFP-Bsd) in PBS | EF1a-Null-GB-PBS
GenTarget

CRE (Puro), CMV lentivirus in PBS | LVP339-PBS
GenTarget

CRE (Neo), CMV lentivirus, in PBS | LVP297-PBS
GenTarget

CRE (Neo), CAG lentivirus, in PBS | LVP575-PBS
GenTarget

CamKII-Luc (Bsd) Lentivirus in PBS | LVP1055-B-PBS
GenTarget

ARE-GFP (Bsd) Lentivirus in PBS | LVP981-B-PBS
GenTarget

AP1-SEAP (Puro) Lentivirus in PBS | LVP1280-PBS
GenTarget

AP1-Luc (Neo) Lentivirus in PBS | LVP955-N-PBS
GenTarget


PureFection™ Transfection reagent
System Biosciences


PureFection Transfection Reagent
System Biosciences


pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
System Biosciences


pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
System Biosciences
![pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM plasmid] pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM plasmid]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14476/14744/sbi%20system%20biosciences_1633075021__00205.original__26154.1633076848.jpg?c=1)
![pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM plasmid] pLL-EF1a-RFP-T2A-Puro [Lenti-LabelerTM plasmid]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14476/30003/LL210PA__01215.1640249171.png?c=1)
pLL-EF1a-RFP-T2A-Puro [Lenti-Labeler™ plasmid]
System Biosciences


piggyBac qPCR copy number kit, 20 reactions
System Biosciences


pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector
System Biosciences


PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector
System Biosciences


PB-EF1a-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC Vector
System Biosciences


PB-CMV-miR-302/367-EF1-GFP iPSC Vector
System Biosciences

Mouse pre-microRNA Expression Construct mir-210
System Biosciences

miRZip-210 anti-miR-210 microRNA construct
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


Exosome-Depleted FBS Media Supplement
System Biosciences


Constitutive (CAGs promoter) EEV cloning and expression vector
System Biosciences

"S" SARS-CoV-2 Spike Glycoprotein mRNA (20 ug, Transfection-ready)
System Biosciences

Tet-on circRNA Expression Vector Plasmid | PVT14643
Nova Lifetech

Super PiggyBac Transposase (PB200PA- 1) | PVT10877
Nova Lifetech

PiggyBac Dual promoter-SHCBP1 Plasmid | PVT13403
Nova Lifetech