Vectormaps
I.M.A.G.E. Consortium
“Sharing resources to achieve a common goal — the discovery of all genes”
Vectors
1. Lafmid BA
2. pAD-GAL4
3. pAMP1
4. pAMP10
5. pBC SK+
6. pBK-CMV
7. pBluescribe (modified)
8. pBluescript (modified)
9. pBluescript II KS+
10. pBluescript II SK+
11. pBluescript KS+
12. pBluescript SK+
13. pBluescript SK-
14. pBluescript-FL
15. pBluescriptR
16. pBSRN3
17. pCDNA3
18. pcDNA3.1
19. pcDNA3.1(-)
20. pcDNA3.1Zeo
21. pcDNAI
22. pCDNAII
23. pCI-neo (updated)
24. pCMV-SPORT
25. pCMV-SPORT2
26. pCMV-SPORT4
27. pCMV-SPORT6
28. pCMV-SPORT6.1
29. pCMV-SPORT6.ccdb
30. pCR-BluntII-TOPO
31. pCR-XL-TOPO
32. pCR2.1-TOPO
33. pCR3.1
34. pCR4-TOPO
35. pCR4Blunt-TOPO
36. pCS105
37. pCS107
38. pCS108
39. pCS111
40. pCS2+
41. pCS22+
42. pCS2G
43. pDeliver1.1
44. pDNR-Dual
45. pDNR-LIB
46. pDNR-NCI
47. pDONR201
48. pDONR223
49. pENTR/D-TOPO
50. pENTR201
51. pENTR221
52. pENTR223
53. pENTR223.1
54. pENTR223.1-LC
55. pENTR223.1-Sfi
56. pET-28a
57. pET-28b
58. pExpress-1
59. pFLC1
60. pGA1
61. pGA14
62. pGA15
63. pGA18
64. pGA19
65. pGA24
66. pGA4
67. pGA7
68. pGH
69. pMA
70. pME18S-FL3
71. pMK
72. pMK-U
73. pOTB7
74. pOTB7-3
75. pOTB7a
76. pPCR-Script Amp SK(+)
77. pRKW2
78. pSMART_cDNA
79. pSPORT1
80. pSPORT2
81. pT7T3D-PacI
82. pUC19
83. pUC19-Cam
84. pUC19-Kan
85. pUC19-Sfi
86. pUC57
87. pYX-Asc
88. pZERO-2
89. pZL-1
Note: The sequencing primers listed below for each vector are not exhaustive (only the most commonly used primers have been checked, -21M13, M13 reverse, t7, t3, and sp6) and others not listed may also be suitable. We also recommend that you check the exact sequence of your primer against the vector sequence to ensure that it matches, as there are often several slightly different versions of these common primers. When custom primers are required, their sequences are listed.
Lafmid BA
Name: Lafmid BA
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Columbia University/Soares
Vector type: phagemid
Vector Map: Available Here
Comments: FLANKING PCR PRIMERS: ab1: gaattgtgagcggataac ab2: gttttcccagtcacgacg or dm1: agctatgaccatgattacgcca dm2: gacggccagtgaattcccct. Annealing Temp: 55C
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
taacaatttcacacaggaaacagctatgaccatgattacgccaagcttgg
ctcgacggatccccttgcggccgcaaggggaattccactggccgtcgttt
tacaacgtcgtgactgggaaaaccc
Full Sequence:
cgatgaacggtaatcgtaaaactagcatgtcaatcatatgtaccccggtt
gataatcagaaaagccccaaaaacaggaagattgtataagcaaatattta
aattgtaaacgttaatattttgttaaaattcgcgttaaatttttgttaaa
tcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaa
tcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaa
gagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccg
tctatcagggcgatggcccactacgtgaaccatcaccctaatcaagtttt
ttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagccc
ccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaag
ggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtc
acgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacaggg
cgcgtactatggttgctttgacgagcacgtataacgtgctttcctcgtta
gaatcagagcgggagctaaacaggaggccgattaaagggattttagacag
gaacggtacgccagaatcctgagaagtgtttttataatcagtgaggccac
cgagtaaaagagtctgtccatcacgcaaattaaccgttgtcgcaatactt
ctttgattagtaataacatcacttgcctgagtagaagaactcaaactatc
ggccttgctggtaatatccagaacaatattaccgccagccattgcaacag
gaaaaacgctcatggaaatacctacattttgacgctcaatcgtctggaat
tnccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcg
ggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggc
gattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccagtgaattccccttgcggccgcaaggggatccgtcgagccaagc
ttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgct
cacaattccacacaacatacgagccggaagcataaagtgtaaagcctggg
gtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgccc
gctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggcca
acgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgc
tcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagct
cactcaaaggcggtaatacggttatccacagaatcaggggataacgcagg
aaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg
ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcac
aaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaag
ataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccga
ccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtg
gcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgt
tcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct
gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgac
ttatcgccactggcagcagccactggtaacaggattagcagagcgaggta
tgtaggcggtgctacagagttcttgaagtggtggcctaactacggctaca
ctagaaggacagtatttggtatctgcgctctgctgaagccagttaccttc
ggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtag
cggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat
ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaac
gaaaactcacgttaagggattttggtcatgagattatcaaaaaggatctt
cacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagta
tatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccc
cgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg
ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca
ataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagta
gttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatc
gtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttccca
acgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtta
gctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgtta
tcactcatggttatggcagcactgcataattctcttactgtcatgccatc
cgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgag
aatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat
aataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacg
ttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagtt
cgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttc
accagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaa
gggaataagggcgacacggaaatgttgaatactcatactcttcctttttc
aat
pAD-GAL4
Name: pAD-GAL4
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Stratagene
Vector type: phagemid
Vector Map: Available Here
Polylinker Sequence:
ccaaacccaaaaaaagagatcgaattcgcccgggcctcgagcccgggtcg
actctagagccctatagtgagtcgtattactgcagagatctatgaatcgt
agatactgaaaaac
pAMP1
Name: pAMP1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
gattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccagtgaattgaatttaggtgacactatagaagagctatgacgtcg
catgcacgcgtacgtaagcttggatcctctagagcggccgcctactacta
ctaatgatgatgatgcccgggaattccggaccggtacctgcaggcgtacc
agctttccctatagtgagtcgtattagagcttggcgtaatcatggtcata
gctgtttcctgtgtgaaattgttatccgctcacaattccacaca
Full Sequence:
cattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggc
ctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat
taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacg
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagcggccgcctactactacta
atgatgatgatgcccgggaattccggaccggtacctgcaggcgtaccagc
tttccctatagtgagtcgtattagagcttggcgtaatcatggtcatagct
gtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgag
ccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactc
acattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtc
gtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgc
gtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacag
ctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtcca
cgctggtttgccccagcaggcgaaaatcctgtttgatggtggttgacggc
gggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagat
atccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgccca
gcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctca
ttcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcc
ttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgcc
agccagccagacgcagacgcgccgagacagaacttaatgggcccgctaac
agcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcg
cgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcag
agacatcaagaaataacgccggaacattagtgcaggcagcttccacagca
atggcatcctggtcatccagcggatagttaatgatcagcccactgacccg
ttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttc
gttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagat
ttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggt
ggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgc
ggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgc
gttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctg
ataagagacaccggcatactctgcgacatcgtataacgttactggtttca
cattcaccaccctgaattgactctcttccgggcgctatcatgccataccg
cgaaaggttttgcgccattcgatggtgtcaacgtaaatgccgcttcgcct
tcgcgcgcgaattgcaagctctgcattaatgaatcggccaacgcgcgggg
agaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactc
gctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaagg
cggtaatacggttatccacagaatcaggggataacgcaggaaagaacatg
tgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgct
ggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac
gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcg
tttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgct
taccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctc
aatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaag
ctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatc
cggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac
tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggt
gctacagagttcttgaagtggtggcctaactacggctacactagaaggac
agtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagag
ttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttt
tttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaga
tcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac
gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatc
cttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagta
aacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcag
cgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtag
ataactacgatacgggagggcttaccatctggccccagtgctgcaatgat
accgcgagacccacgctcaccggctccagatttatcagcaataaaccagc
cagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcc
atccagtctattaattgttgccgggaagctagagtaagtagttcgccagt
taatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcac
gctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaagg
cgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcgg
tcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatgg
ttatggcagcactgcataattctcttactgtcatgccatccgtaagatgc
ttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtat
gcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgc
cacatagcagaactttaaaagtgctcatcattggaaaacgttcttcgggg
cgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacc
cactcgtgcacccaactgatcttcagcatcttttactttcaccagcgttt
ctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagg
gcgacacggaaatgttgaatactcatactcttcctttttcaatattattg
aagcatttatcagggttattgtctcatgagcggatacatatttgaatgta
tttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtg
ccacctgaaattgtaaacgttaatattttgttaaaattcgcgttaaattt
ttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcc
cttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtt
tggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcg
aaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaat
caagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaa
gggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgag
aaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtg
tagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccg
ctacagggcgcgtc
pAMP10
Name: pAMP10
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
cccagtcacgacgttgtaaaacgacggccagtgaattgaatttaggtgac
actatagaagagctatgacgtcgcatgcacgcgtacgtaagcttggatcc
tctagagcggccggccgcctactactactaatgatgatgatgtcgacccg
ggaattccggaccggtacctgcaggcgtaccagctttccctatagtgagt
cgtattagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaat
tgttatc
Full Sequence:
cattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggc
ctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat
taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacg
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagcggccggccgcctactact
actaatgatgatgatgtcgacccgggaattccggaccggtacctgcaggc
gtaccagctttccctatagtgagtcgtattagagcttggcgtaatcatgg
tcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaa
catacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtga
gctaactcacattaattgcgttgcgctcactgcccgctttccagtcggga
aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagagg
cggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacg
ggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaa
gcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtgg
ttgacggcgggatataacatgagctgtcttcggtatcgtcgtatcccact
accgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcat
tgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacga
tgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactc
cagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagata
tttatgccagccagccagacgcagacgcgccgagacagaacttaatgggc
ccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacg
cccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgt
ctggtcagagacatcaagaaataacgccggaacattagtgcaggcagctt
ccacagcaatggcatcctggtcatccagcggatagttaatgatcagccca
ctgacccgttgcgcgagaagattgtgcaccgccgctttacaggcttcgac
gccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcgg
cgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccaga
ctggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttg
tgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactt
tttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaa
acggtctgataagagacaccggcatactctgcgacatcgtataacgttac
tggtttcacattcaccaccctgaattgactctcttccgggcgctatcatg
ccataccgcgaaaggttttgcgccattcgatggtgtcaacgtaaatgccg
cttcgccttcgcgcgcgaattgcaagctctgcattaatgaatcggccaac
gcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctc
actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctca
ctcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa
agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggcc
gcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaa
aaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagat
accaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc
ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggc
gctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttc
gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgc
gccttatccggtaactatcgtcttgagtccaacccggtaagacacgactt
atcgccactggcagcagccactggtaacaggattagcagagcgaggtatg
taggcggtgctacagagttcttgaagtggtggcctaactacggctacact
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgg
aaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg
gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatct
caagaagatcctttgatcttttctacggggtctgacgctcagtggaacga
aaactcacgttaagggattttggtcatgagattatcaaaaaggatcttca
cctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata
tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacc
tatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg
tcgtgtagataactacgatacgggagggcttaccatctggccccagtgct
gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaat
aaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttat
ccgcctccatccagtctattaattgttgccgggaagctagagtaagtagt
tcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgt
ggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaac
gatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagc
tccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatc
actcatggttatggcagcactgcataattctcttactgtcatgccatccg
taagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaa
tagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataa
taccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgtt
cttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcg
atgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac
cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagg
gaataagggcgacacggaaatgttgaatactcatactcttcctttttcaa
tattattgaagcatttatcagggttattgtctcatgagcggatacatatt
tgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccc
gaaaagtgccacctgaaattgtaaacgttaatattttgttaaaattcgcg
ttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcgg
caaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttg
ttccagtttggaacaagagtccactattaaagaacgtggactccaacgtc
aaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatc
accctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcgga
accctaaagggagcccccgatttagagcttgacggggaaagccggcgaac
gtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgct
ggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgctta
atgcgccgctacagggcgcgtc
pBC SK+
Name: pBC SK+
Antibiotic: chloramphenicol
Concentration: 25 ug/ml
Source: Stratagene
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCG
AATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGAT
ATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCAC
CGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGC
TTGGCGTAATCATGGTCATAGCTGTTTCC
Full Sequence:
CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTT
AAA
pBK-CMV
Name: pBK-CMV
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: Stratagene
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
aaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttc
ccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcac
tatagggcgaattgggtacacttacctggtaccccacccgggtggaaaat
cgatgggcccgcggccgctctagaagtactctcgagaagctttttgaatt
ctttggatccactagtgtcgacctgcaggcgcgcgagctccagcttttgt
tccctttagtgagggttaatttcgagcttggcgtaatcaaggtcatagct
gtttcctgtgtgaaattgttatccgctcacaattccacacaatatacgag
ccggaagtataaagtgtaaagcctggggtgccta
Full Sequence:
pBluescribe (modified)
Name: pBluescribe (modified)
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Jackson Laboratory
Vector type: phagemid
Vector Map: Available Here
Comments: Stratagene vector modified by Knowles/Solter (Jackson Laboratory): original EcoRI site changed to MluI, original HindIII site changed to SalI.
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
taatacgactcactatagggatcactatagggcgaattgacgcgtg...5
' end of insert (Knowles/Solter
libraries).........3' end of
insert...gtcgaccggagcttttgttccctttagtgagggttaat
Full Sequence:
aaaaaacggtcgaccggagcttttgttccctttagtgagggttaattccg
agcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatcc
gctcacaattccacacaacatacgagccggaagcataaagtgtaaagcct
ggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactg
cccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcgg
ccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcct
cgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatca
gctcactcaaaggcggtaatacggttatccacagaatcaggggataacgc
aggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaa
aggccgcgttgctggcgtttttccataggctccgcccccctgacgagcat
cacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactata
aagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttc
cgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagc
gtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggt
cgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgacc
gctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacac
gacttatcgccactggcagcagccactggtaacaggattagcagagcgag
gtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct
acactagaaggacagtatttggtatctgcgctctgctgaagccagttacc
ttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctgg
tagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaag
gatctcaagaagatcctttgatcttttctacggggtctgacgctcagtgg
aacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggat
cttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaa
gtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgag
gcacctatctcagcgatctgtctatttcgttcatccatagttgcctgact
ccccgtcgtgtagataactacgatacgggagggcttaccatctggcccca
gtgctgcaatgataccgcgagacccacgctcaccggctccagatttatca
gcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaac
tttatccgcctccatccagtctattaattgttgccgggaagctagagtaa
gtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggc
atcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttc
ccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcgg
ttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtg
ttatcactcatggttatggcagcactgcataattctcttactgtcatgcc
atccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattct
gagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgg
gataataccgcgccacatagcagaactttaaaagtgctcatcattggaaa
acgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcca
gttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttact
ttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaa
aaagggaataagggcgacacggaaatgttgaatactcatactcttccttt
ttcaatattattgaagcatttatcagggttattgtctcatgagcggatac
atatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatt
tccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacat
taacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttc
ggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcac
agcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgt
cagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagag
cagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatg
cgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgca
actgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctg
gcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggtt
ttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgact
cactatagggatcactatagggcgaattgacgcgtga
pBluescript (modified)
Name: pBluescript (modified)
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: UCLA/Lin
Vector type: phagemid
Comments: custom modification from original Stratagene vector
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
ttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcg
aattgggtaccgggccccccctcgaggtcgacggtatcgataagcttgat
atcgaattccactgcgtgctgcgcccgggggatccgcatgccacactgtg
tctagagcggccgccaccgcggtggagctccagttttgttccctttagtg
agggttaattgcgcgcttggcgtaatcatggtcatagctgtttcc
pBluescript II KS+
Name: pBluescript II KS+
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Stratagene
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
ggaaacagctatgaccatgattacgccaagctcgaaattaaccctcacta
aagggaacaaaagctggagctccaccgcggtggcggccgctctagaacta
gtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtatt
acaattcactggccgtcgttttacaa
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat
tggagctccaccgcggtggcggccgctctagaactagtggatcccccggg
ctgcaggaattcgatatcaagcttatcgataccgtcgacctcgagggggg
gcccggtacccagcttttgttccctttagtgagggttaattgcgcgcttg
gcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcac
aattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgct
ttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg
cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctca
ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac
tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg
cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg
ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg
ccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta
tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga
aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa
aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgt
cgtgtagataactacgatacgggagggcttaccatctggccccagtgctg
caatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc
cgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt
cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg
atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct
ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatca
ctcatggttatggcagcactgcataattctcttactgtcatgccatccgt
aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat
accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga
tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg
aataagggcgacacggaaatgttgaatactcatactcttcctttttcaat
attattgaagcatttatcagggttattgtctcatgagcggatacatattt
gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg
aaaagtgccac
pBluescript II SK+
Name: pBluescript II SK+
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Stratagene
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
GCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCT
ACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGC
GATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGT
GCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACG
TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCG
AATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGAT
ATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCAC
CGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGC
TTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT
CACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGG
GTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCC
GCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCA
ACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGC
TCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCT
CACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC
Full Sequence:
CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTT
AAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTAT
AAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAA
CAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAA
CCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGT
TTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG
CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGG
AAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCG
GTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACA
GGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGAT
CGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCT
GCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAAT
TGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATC
GAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGC
GGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTG
GCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC
AATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
CCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCT
TTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
CGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCA
CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCAC
TCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAA
GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA
AATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATA
CCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCG
CTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCG
CTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTA
TCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGT
AGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA
AAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGG
TGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAA
AACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCAC
CTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATAT
ATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCT
ATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGT
CGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTG
CAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATA
AACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATC
CGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTT
CGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTG
GTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACG
ATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCT
CCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCA
CTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGT
AAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAAT
AGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAAT
ACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTC
TTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGA
TGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACC
AGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGG
AATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAT
ATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTT
GAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCG
AAAAGTGCCAC
pBluescript KS+
Name: pBluescript KS+
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Stratagene
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
CGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCT
GCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAAT
TGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGG
CTGCAGGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGG
GCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTG
GCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC
AATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
CCTAATGAGTGAGCTA
Full Sequence:
CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTT
AAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTAT
AAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAA
CAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAA
CCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGT
TTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG
CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGG
AAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCG
GTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACA
GGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGAT
CGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCT
GCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAAT
TGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGG
CTGCAGGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGG
GCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTG
GCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC
AATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
CCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCT
TTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACG
CGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCA
CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCAC
TCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAA
GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA
AATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATA
CCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCG
CTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCG
CTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTA
TCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGT
AGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA
AAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGG
TGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAA
AACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCAC
CTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATAT
ATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCT
ATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGT
CGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTG
CAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATA
AACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATC
CGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTT
CGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTG
GTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACG
ATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCT
CCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCA
CTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGT
AAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAAT
AGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAAT
ACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTC
TTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGA
TGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACC
AGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGG
AATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAT
ATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTT
GAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCG
AAAAGTGCCAC
pBluescript SK+
Name: pBluescript SK+
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Stratagene
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
ggaaacagctatgaccatgattacgccaagctcgaaattaaccctcacta
aagggaacaaaagctggagctccaccgcggtggcggccgctctagaacta
gtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtatt
acaattcactggccgtcgttttacaa
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat
tgggtaccgggccccccctcgaggtcgacggtatcgataagcttgatatc
gaattcctgcagcccgggggatccactagttctagagcggccgccaccgc
ggtggagctccagcttttgttccctttagtgagggttaattgcgcgcttg
gcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcac
aattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgct
ttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg
cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctca
ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac
tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg
cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg
ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg
ccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta
tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga
aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa
aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgt
cgtgtagataactacgatacgggagggcttaccatctggccccagtgctg
caatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc
cgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt
cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg
atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct
ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatca
ctcatggttatggcagcactgcataattctcttactgtcatgccatccgt
aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat
accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga
tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg
aataagggcgacacggaaatgttgaatactcatactcttcctttttcaat
attattgaagcatttatcagggttattgtctcatgagcggatacatattt
gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg
aaaagtgccac
pBluescript SK-
Name: pBluescript SK-
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Stratagene
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
ggaaacagctatgaccatgattacgccaagctcgaaattaaccctcacta
aagggaacaaaagctggagctccaccgcggtggcggccgctctagaacta
gtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtatt
acaattcactggccgtcgttttacaa
Full Sequence:
ctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacg
cgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgc
tttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctc
taaatcgggggctccctttagggttccgatttagtgctttacggcacctc
gaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgcc
ctgatagacggtttttcgccctttgacgttggagtccacgttctttaata
gtggactcttgttccaaactggaacaacactcaaccctatctcggtctat
tcttttgatttataagggattttgccgatttcggcctattggttaaaaaa
tgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgc
ttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat
tgggtaccgggccccccctcgaggtcgacggtatcgataagcttgatatc
gaattcctgcagcccgggggatccactagttctagagcggccgccaccgc
ggtggagctccagcttttgttccctttagtgagggttaattgcgcgcttg
gcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcac
aattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgct
ttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg
cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctca
ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac
tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg
cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg
ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg
ccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta
tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga
aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa
aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgt
cgtgtagataactacgatacgggagggcttaccatctggccccagtgctg
caatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc
cgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt
cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg
atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct
ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatca
ctcatggttatggcagcactgcataattctcttactgtcatgccatccgt
aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat
accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga
tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg
aataagggcgacacggaaatgttgaatactcatactcttcctttttcaat
attattgaagcatttatcagggttattgtctcatgagcggatacatattt
gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg
aaaagtgccac
pBluescript-FL
Name: pBluescript-FL
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: University of Tokyo/Sugano lab
Vector type: phagemid
Has Custom Primer: yes
Vector Map: Available Here
Comments: NOTE [previously located in "full_seq" table]: the polylinker sequence above replaces the following sequence in pBluescript: actagtggatcccccgggctgcag
Sequencing Primers: t7, t3
Polylinker Sequence:
actagtggatccattgtttggccttgttggccatcatttgggctgcag
Full Sequence:
CTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACG
CGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGC
TTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTC
TAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTC
GACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCC
CTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATA
GTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTAT
TCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAA
TGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGC
TTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGAT
CGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCT
GCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAAT
TGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCATTGTT
TGGCCTTGTTGGCCATCATTTGGGCTGCAGGAATTCGATATCAAGCTTAT
CGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTT
TAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCC
TGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAA
GCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTA
ATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCA
GCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTG
GGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC
AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTC
CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCG
AAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCC
TTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG
TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAA
CAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCT
CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG
CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACG
GGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCAT
GAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAA
GTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTAC
CAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTC
ATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGG
GCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCA
CCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCG
CAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTT
GCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTT
GTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGC
TTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCA
TGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGA
AGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAA
TTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGT
ACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCT
TGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAA
AGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCT
TACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGA
TCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGG
AAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAA
TACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTAT
TGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAAT
AGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC
pBluescriptR
Name: pBluescriptR
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Brownstein
Vector type: phagemid
Vector Map: Available Here
Comments: modified pBluescript from Riken.
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
tgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaa
agggggatgtgctgcaaggcgattaagttgggtaacgccagggttttccc
agtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcacta
tagggcgaattggagctccaccgcggtggcggccgcataacttcgtatag
catacattatacgaagttatggatcaggccaaatcggccgagctcgaatt
cgtcgag----5'-insert-3'----ggatccggccataagggcctgat
ccgtcgagggggggcccggtaccagcttttgttccctttagtgagggtta
atttcgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattg
ttatccgctcacaattccacacaacatacgagccggaagcataaagtgta
aagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgc
tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatg
aatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccg
cttcctcgctcactgactcgctgcgctcgg
Full Sequence:
cacctaaattgtaagcgttaatattttgttaaaattcgcgttaaattttt
gttaaatcagctcattttttaaccaataggccgaaatcggcaaaatccct
tataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttg
gaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaa
aaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatca
agttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagg
gagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaa
aggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgta
gcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgct
acagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggc
gatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgt
gctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacg
ttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaat
tggagctccaccgcggtggcggccgcataacttcgtatagcatacattat
acgaagttatggatcaggccaaatcggccgagctcgaattcgtcgag---
-"-insert-"----ggatccggccataagggcctgatccgtcgaggggg
ggcccggtaccagcttttgttccctttagtgagggttaatttcgagcttg
gcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcac
aattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgct
ttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg
cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctca
ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac
tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg
cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg
ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg
ccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta
tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga
aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa
aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgt
cgtgtagataactacgatacgggagggcttaccatctggccccagtgctg
caatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc
cgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt
cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg
atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct
ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatca
ctcatggttatggcagcactgcataattctcttactgtcatgccatccgt
aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat
accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga
tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg
aataagggcgacacggaaatgttgaatactcatactcttcctttttcaat
attattgaagcatttatcagggttattgtctcatgagcggatacatattt
gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg
aaaagtgc
pBSRN3
Name: pBSRN3
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Wellcome Trust
Vector type: plasmid
Has Custom Primer: yes
Comments: modified pBluescript KS+, REFERENCE: Lemaire, et al., Cell (1995) 81(1)85-94.
Polylinker Sequence:
gagctaagcttgcttgttctttttgcagaagctcagaataaacgctcaac
tttggcagatctgaattccccggggcggccgcggatctggttaccactaa
accagcctcaagaacacccgaatggagtctctaagctacataataccaac
ttacactttacaaaatgttgtcccccaaaatgtagccattcgtatctgct
cctaataaaaagaaagtttcttcacttctaaaaa
pCDNA3
Name: pCDNA3
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Polylinker Sequence:
ATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTA
CGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT
ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATT
GACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACAT
CAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAA
ATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTA
CTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGG
TTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATT
TCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAA
ATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAA
ATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTG
GCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTC
ACTATAGGGAGACCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGG
CCGCCAGTGTGCTGGAATTCTGCAGATATCCATCACACTGGCGGCCGCTC
GAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAG
CTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTT
TGCCCCTCCCCCGTGCC
Full Sequence:
GACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATC
TGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTT
GGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAG
GCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCG
CTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGAC
TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA
TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG
CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGT
AACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGT
AAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCC
CCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA
CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCA
TCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGA
TAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA
TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTA
ACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAG
GTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTG
GCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTGGTACC
GAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGAT
ATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCT
ATAGTGTCACCTAAATGCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTT
CTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACC
CTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGC
ATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGC
AGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGAT
GCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAG
GGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG
TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT
CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG
TCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTAC
GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG
CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT
CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT
CGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGG
TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGG
AATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGGCAGGCA
GAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAG
TCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTA
GTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTC
CGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATT
TATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTG
AGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTT
GTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGC
ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGA
GAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATG
CCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAG
ACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCT
ATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTG
TCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAG
GATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGC
TGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCG
ACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCC
GGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCC
AGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATC
TCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAAT
GGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCG
CTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCG
GCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGAT
TCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGG
ACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACG
AGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCG
TTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTG
GAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAA
ATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGC
ATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGT
ATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTT
TCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCG
GAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACA
TTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTG
CCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA
TTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTT
CGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATC
CACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGG
CTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG
GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCC
GCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAG
GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTT
GAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGC
GCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATC
CGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCT
ACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGT
CATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAAT
GAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGT
TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCG
TTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGG
AGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGC
TCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGA
GCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATT
GTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAAC
GTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTAT
GGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCC
CCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTC
AGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCA
TAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTG
AGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGC
TCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTT
AAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA
TCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAAC
TGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAAC
AGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTT
GAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGT
TATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACA
AATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC
pcDNA3.1
Name: pcDNA3.1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Has Custom Primer: yes
Vector Map: Available Here
Sequencing Primers: t7, sp6
Polylinker Sequence:
agtacatcaatgggcgtggatagcggtttgactcacggggatttccaagt
ctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacg
ggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcg
gtaggcgtgtacggtgggaggtctatataagcagagctctctggctaact
agagaacccactgcttactggcttatcgaaattaatacgactcactatag
ggagacccaagcttggtaccgagctcggatccactagtaacggccgccag
tgtgctggaattctgcagatatccatcacactggcggccgctcgagcatg
catctagagggccctattctatagtgtcacctaaatgctagagctcgctg
atcagcctcgactgtgcctctagttgccagccatctgttgtttgcccctc
ccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcct
aataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctatt
ctggggggtggggtggggcaggacagcaagggggaggattgggaagacaa
tagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaa
gaaccagctggggctctagggggtatccccacgcgccctgtagcggcgca
ttaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgc
cagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgcca
cgttcgccggctttccccgtcaagctctaaatcggggca
Full Sequence:
gacggatcgggagatctcccgatcccctatggtcgactctcagtacaatc
tgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgtt
ggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaag
gcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcg
ctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgac
tagttattaatagtaatcaattacggggtcattagttcatagcccatata
tggagttccgcgttacataacttacggtaaatggcccgcctggctgaccg
cccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagt
aacgccaatagggactttccattgacgtcaatgggtggactatttacggt
aaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccc
cctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta
catgaccttatgggactttcctacttggcagtacatctacgtattagtca
tcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtgga
tagcggtttgactcacggggatttccaagtctccaccccattgacgtcaa
tgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgta
acaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggag
gtctatataagcagagctctctggctaactagagaacccactgcttactg
gcttatcgaaattaatacgactcactatagggagacccaagcttggtacc
gagctcggatccactagtaacggccgccagtgtgctggaattctgcagat
atccatcacactggcggccgctcgagcatgcatctagagggccctattct
atagtgtcacctaaatgctagagctcgctgatcagcctcgactgtgcctt
ctagttgccagccatctgttgtttgcccctcccccgtgccttccttgacc
ctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgc
atcgcattgtctgagtaggtgtcattctattctggggggtggggtggggc
aggacagcaagggggaggattgggaagacaatagcaggcatgctggggat
gcggtgggctctatggcttctgaggcggaaagaaccagctggggctctag
ggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtgg
tggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgct
cctttcgctttcttcccttcctttctcgccacgttcgccggctttccccg
tcaagctctaaatcggggcatccctttagggttccgatttagtgctttac
ggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtggg
ccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt
ctttaatagtggactcttgttccaaactggaacaacactcaaccctatct
cggtctattcttttgatttataagggattttggggatttcggcctattgg
ttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtgg
aatgtgtgtcagttagggtgtggaaagtccccaggctccccaggcaggca
gaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaag
tccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaatta
gtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactc
cgcccagttccgcccattctccgccccatggctgactaattttttttatt
tatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtg
aggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagctt
gtatatccattttcggatctgatcaagagacaggatgaggatcgtttcgc
atgattgaacaagatggattgcacgcaggttctccggccgcttgggtgga
gaggctattcggctatgactgggcacaacagacaatcggctgctctgatg
ccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaag
accgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggct
atcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttg
tcactgaagcgggaagggactggctgctattgggcgaagtgccggggcag
gatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggc
tgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcg
accaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagcc
ggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgcc
agccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatc
tcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaat
ggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccg
ctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcg
gcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgat
tcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcggg
actctggggttcgaaatgaccgaccaagcgacgcccaacctgccatcacg
agatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcg
ttttccgggacgccggctggatgatcctccagcgcggggatctcatgctg
gagttcttcgcccaccccaacttgtttattgcagcttataatggttacaa
ataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgc
attctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgt
ataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtt
tcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccg
gaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcaca
ttaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtg
ccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgta
ttgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt
cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc
cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagc
aaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagg
ctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg
gcgaaacccgacaggactataaagataccaggcgtttccccctggaagct
ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc
gcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtag
gtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacg
aaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt
gagtccaacccggtaagacacgacttatcgccactggcagcagccactgg
taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttga
agtggtggcctaactacggctacactagaaggacagtatttggtatctgc
gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagc
agattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttct
acggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt
catgagattatcaaaaaggatcttcacctagatccttttaaattaaaaat
gaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagt
taccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcg
ttcatccatagttgcctgactccccgtcgtgtagataactacgatacggg
agggcttaccatctggccccagtgctgcaatgataccgcgagacccacgc
tcaccggctccagatttatcagcaataaaccagccagccggaagggccga
gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaatt
gttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaac
gttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtat
ggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccc
ccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtc
agaagtaagttggccgcagtgttatcactcatggttatggcagcactgca
taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtg
agtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgc
tcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttt
aaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaagga
tcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaac
tgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaac
aggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgtt
gaatactcatactcttcctttttcaatattattgaagcatttatcagggt
tattgtctcatgagcggatacatatttgaatgtatttagaaaaataaaca
aataggggttccgcgcacatttccccgaaaagtgccacctgacgtc
pcDNA3.1(-)
Name: pcDNA3.1(-)
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13 reverse, t7
Polylinker Sequence:
atgtacgggccagatatacgcgttgacattgattattgactagttattaa
tagtaatcaattacggggtcattagttcatagcccatatatggagttccg
cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacc
cccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaata
gggactttccattgacgtcaatgggtggagtatttacggtaaactgccca
cttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacg
tcaatgacggtaaatggcccgcctggcattatgcccagtacatgacctta
tgggactttcctacttggcagtacatctacgtattagtcatcgctattac
catggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttg
actcacggggatttccaagtctccaccccattgacgtcaatgggagtttg
ttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgc
cccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataa
gcagagctctctggctaactagagaacccactgcttactggcttatcgaa
attaatacgactcactatagggagacccaagctggctagcgtttaaacgg
gccctctagactcgagcggccgccactgtgctggatatctgcagaattcc
accacactggactagtggatccgagctcggtaccaagcttaagtttaaac
cgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttg
cccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcc
tttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcat
tctattctggggggtggggtggggcaggacagcaagggggaggattggga
agacaatagcaggcatgctggggatgcggtgggctctatggcttctgagg
Full Sequence:
gacggatcgggagatctcccgatcccctatggtgcactctcagtacaatc
tgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgtt
ggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaag
gcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcg
ctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgac
tagttattaatagtaatcaattacggggtcattagttcatagcccatata
tggagttccgcgttacataacttacggtaaatggcccgcctggctgaccg
cccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagt
aacgccaatagggactttccattgacgtcaatgggtggagtatttacggt
aaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccc
cctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta
catgaccttatgggactttcctacttggcagtacatctacgtattagtca
tcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtgga
tagcggtttgactcacggggatttccaagtctccaccccattgacgtcaa
tgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgta
acaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggag
gtctatataagcagagctctctggctaactagagaacccactgcttactg
gcttatcgaaattaatacgactcactatagggagacccaagctggctagc
gtttaaacgggccctctagactcgagcggccgccactgtgctggatatct
gcagaattccaccacactggactagtggatccgagctcggtaccaagctt
aagtttaaaccgctgatcagcctcgactgtgccttctagttgccagccat
ctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccact
cccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgag
taggtgtcattctattctggggggtggggtggggcaggacagcaaggggg
aggattgggaagacaatagcaggcatgctggggatgcggtgggctctatg
gcttctgaggcggaaagaaccagctggggctctagggggtatccccacgc
gccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcg
tgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttc
ccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcg
ggggctccctttagggttccgatttagtgctttacggcacctcgacccca
aaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatag
acggtttttcgccctttgacgttggagtccacgttctttaatagtggact
cttgttccaaactggaacaacactcaaccctatctcggtctattcttttg
atttataagggattttgccgatttcggcctattggttaaaaaatgagctg
atttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagtta
gggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcat
gcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccag
caggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtc
ccgcccctaactccgcccatcccgcccctaactccgcccagttccgccca
ttctccgccccatggctgactaattttttttatttatgcagaggccgagg
ccgcctctgcctctgagctattccagaagtagtgaggaggcttttttgga
ggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcgg
atctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatg
gattgcacgcaggttctccggccgcttgggtggagaggctattcggctat
gactgggcacaacagacaatcggctgctctgatgccgccgtgttccggct
gtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtg
ccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacg
acgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaag
ggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctc
accttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcgg
ctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaaca
tcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcagg
atgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgcc
aggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatgg
cgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggat
tcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcg
ttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccg
cttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgcct
tctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaa
tgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccacc
gccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccgg
ctggatgatcctccagcgcggggatctcatgctggagttcttcgcccacc
ccaacttgtttattgcagcttataatggttacaaataaagcaatagcatc
acaaatttcacaaataaagcatttttttcactgcattctagttgtggttt
gtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctcta
gctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgt
tatccgctcacaattccacacaacatacgagccggaagcataaagtgtaa
agcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgct
cactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatga
atcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgc
ttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg
tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggat
aacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccg
taaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacg
agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga
ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcc
tgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg
gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtg
taggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcc
cgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaa
gacacgacttatcgccactggcagcagccactggtaacaggattagcaga
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaacta
cggctacactagaagaacagtatttggtatctgcgctctgctgaagccag
ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccacc
gctggtagcggtttttttgtttgcaagcagcagattacgcgcagaaaaaa
aggatctcaagaagatcctttgatcttttctacggggtctgacgctcagt
ggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaagg
atcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcta
aagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtg
aggcacctatctcagcgatctgtctatttcgttcatccatagttgcctga
ctccccgtcgtgtagataactacgatacgggagggcttaccatctggccc
cagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat
cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgca
actttatccgcctccatccagtctattaattgttgccgggaagctagagt
aagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacag
gcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggt
tcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcag
tgttatcactcatggttatggcagcactgcataattctcttactgtcatg
ccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatac
gggataataccgcgccacatagcagaactttaaaagtgctcatcattgga
aaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatc
cagttcgatgtaacccactcgtgcacccaactgatcttcagcatctttta
ctttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgca
aaaaagggaataagggcgacacggaaatgttgaatactcatactcttcct
ttttcaatattattgaagcatttatcagggttattgtctcatgagcggat
acatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca
tttccccgaaaagtgccacctgacgtc
pcDNA3.1Zeo
Name: pcDNA3.1Zeo
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13 reverse, t7
Polylinker Sequence:
cccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagt
aacgccaatagggactttccattgacgtcaatgggtggactatttacggt
aaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccc
cctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta
catgaccttatgggactttcctacttggcagtacatctacgtattagtca
tcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtgga
tagcggtttgactcacggggatttccaagtctccaccccattgacgtcaa
tgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgta
acaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggag
gtctatataagcagagctctctggctaactagagaacccactgcttactg
gcttatcgaaattaatacgactcactatagggagacccaagctggctagc
gtttaaacttaagcttggtaccgagctcggatccactagtccagtgtggt
ggaattctgcagatatccagcacagtggcggccgctcgagtctagagggc
ccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagcca
tctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccac
tcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctga
Full Sequence:
gacggatcgggagatctcccgatcccctatggtcgactctcagtacaatc
tgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgtt
ggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaag
gcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcg
ctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgac
tagttattaatagtaatcaattacggggtcattagttcatagcccatata
tggagttccgcgttacataacttacggtaaatggcccgcctggctgaccg
cccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagt
aacgccaatagggactttccattgacgtcaatgggtggactatttacggt
aaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccc
cctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta
catgaccttatgggactttcctacttggcagtacatctacgtattagtca
tcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtgga
tagcggtttgactcacggggatttccaagtctccaccccattgacgtcaa
tgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgta
acaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggag
gtctatataagcagagctctctggctaactagagaacccactgcttactg
gcttatcgaaattaatacgactcactatagggagacccaagctggctagc
gtttaaacttaagcttggtaccgagctcggatccactagtccagtgtggt
ggaattctgcagatatccagcacagtggcggccgctcgagtctagagggc
ccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagcca
tctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccac
tcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctga
gtaggtgtcattctattctggggggtggggtggggcaggacagcaagggg
gaggattgggaagacaatagcaggcatgctggggatgcggtgggctctat
ggcttctgaggcggaaagaaccagctggggctctagggggtatccccacg
cgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagc
gtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttctt
cccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatc
ggggcatccctttagggttccgatttagtgctttacggcacctcgacccc
aaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgata
gacggtttttcgccctttgacgttggagtccacgttctttaatagtggac
tcttgttccaaactggaacaacactcaaccctatctcggtctattctttt
gatttataagggattttggggatttcggcctattggttaaaaaatgagct
gatttaacaaaaatttaacgcgaattaattctgtggaatgtgtgtcagtt
agggtgtggaaagtccccaggctccccaggcaggcagaagtatgcaaagc
atgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctcccc
agcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatag
tcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcc
cattctccgccccatggctgactaattttttttatttatgcagaggccga
ggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttg
gaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttc
ggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcggc
atagtataatacgacaaggtgaggaactaaaccatggccaagttgaccag
tgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttct
ggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgcc
ggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggacca
ggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacg
agctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcc
tccgggccggccatgaccgagatcggcgagcagccgtgggggcgggagtt
cgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgaggagc
aggactgacacgtgctacgagatttcgattccaccgccgccttctatgaa
aggttgggcttcggaatcgttttccgggacgccggctggatgatcctcca
gcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattg
cagcttataatggttacaaataaagcaatagcatcacaaatttcacaaat
aaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaa
tgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgt
aatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaatt
ccacacaacatacgagccggaagcataaagtgtaaagcctggggtgccta
atgagtgagctaactcacattaattgcgttgcgctcactgcccgctttcc
agtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcg
gggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga
ctcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaa
aggcggtaatacggttatccacagaatcaggggataacgcaggaaagaac
atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgtt
gctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccag
gcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgcc
gcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgcttt
ctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcc
aagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctt
atccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgc
cactggcagcagccactggtaacaggattagcagagcgaggtatgtaggc
ggtgctacagagttcttgaagtggtggcctaactacggctacactagaag
gacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaa
gagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggt
ttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaga
agatcctttgatcttttctacggggtctgacgctcagtggaacgaaaact
cacgttaagggattttggtcatgagattatcaaaaaggatcttcacctag
atccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatga
gtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatct
cagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtg
tagataactacgatacgggagggcttaccatctggccccagtgctgcaat
gataccgcgagacccacgctcaccggctccagatttatcagcaataaacc
agccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcc
tccatccagtctattaattgttgccgggaagctagagtaagtagttcgcc
agttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgt
cacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatca
aggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt
cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactca
tggttatggcagcactgcataattctcttactgtcatgccatccgtaaga
tgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtg
tatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccg
cgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcg
gggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgta
acccactcgtgcacccaactgatcttcagcatcttttactttcaccagcg
tttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaata
agggcgacacggaaatgttgaatactcatactcttcctttttcaatatta
ttgaagcatttatcagggttattgtctcatgagcggatacatatttgaat
gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaa
gtgccacctgacgtc
pcDNAI
Name: pcDNAI
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: t7, sp6
Polylinker Sequence:
acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaa
tgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacg
gtgggaggtctatataagcagagctctctggctaactagagaacccactg
cttactggcttatcgaaattaatacgactcactatagggagacccaagct
tggtaccgagctcggatccactagtaacggccgccagtgtgctggaattc
tgcagatatccatcacactggcggccgctcgagcatgcatctagagggcc
ctattctatagtgtcacctaaatgctagaggatctttgtgaaggaacctt
acttctgtggtgtgacataattggacaaactacctacagagatttaaagc
Full Sequence:
ggcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggt
ttgtttgccggatcaagagctaccaactctttttccgaaggtaactggct
tcagcagagcgcagataccaaatactgtccttctagtgtagccgtagtta
ggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgct
aatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccg
ggttggactcaagacgatagttaccggataaggcgcagcggtcgggctga
acggggggttcgtgcacacagcccagcttggagcgaacgacctacaccga
actgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaag
ggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagag
cgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgt
cgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcag
gggggcggagcctatggaaaaacgccagcaacgcaagctagcttctagct
agaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagcccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggccgcccactacgtgaaccatcacccaaatca
agttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagg
gagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaa
aggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgta
gcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgct
acagggcgcgtactatggttgctttgacgagaccgtataacgtgctttcc
tcgttggaatcagagcgggagctaaacaggaggccgattaaagggatttt
agacaggaacggtacgccagctggattaccgcggtctttctcaacgtaac
actttacagcggcgcgtcatttgatatgatgcgccccgcttcccgataag
ggagcaggccagtaaaagcattacccgtggtggggttcccgagcggccaa
agggagcagactctaaatctgccgtcatcgacttcgaaggttcgaatcct
tcccccaccaccatcactttcaaaagtccgaaagaatctgctccctgctt
gtgtgttggaggtcgctgagtagtgcgcgagtaaaatttaagctacaaca
aggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcg
ttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgat
tattgactagttattaatagtaatcaattacggggtcattagttcatagc
ccatatatggagttccgcgttacataacttacggtaaatggcccgcctgg
ctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttc
ccatagtaacgccaatagggactttccattgacgtcaatgggtggactat
ttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaag
tacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatg
cccagtacatgaccttatgggactttcctacttggcagtacatctacgta
ttagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgg
gcgtggatagcggtttgactcacggggatttccaagtctccaccccattg
acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaa
tgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacg
gtgggaggtctatataagcagagctctctggctaactagagaacccactg
cttactggcttatcgaaattaatacgactcactatagggagacccaagct
tggtaccgagctcggatccactagtaacggccgccagtgtgctggaattc
tgcagatatccatcacactggcggccgctcgagcatgcatctagagggcc
ctattctatagtgtcacctaaatgctagaggatctttgtgaaggaacctt
acttctgtggtgtgacataattggacaaactacctacagagatttaaagc
tctaaggtaaatataaaatttttaagtgtataatgtgttaaactactgat
tctaattgtttgtgtattttagattccaacctatggaactgatgaatggg
agcagtggtggaatgcctttaatgaggaaaacctgttttgctcagaagaa
atgccatctagtgatgatgaggctactgctgactctcaacattctactcc
tccaaaaaagaagagaaaggtagaagaccccaaggactttccttcagaat
tgctaagttttttgagtcatgctgtgtttagtaatagaactcttgcttgc
tttgctatttacaccacaaaggaaaaagctgcactgctatacaagaaaat
tatggaaaaatatttgatgtatagtgccttgactagagatcataatcagc
cataccacatttgtagaggttttacttgctttaaaaaacctcccacacct
ccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgt
ttattgcagcttataatggttacaaataaagcaatagcatcacaaatttc
acaaataaagcatttttttcactgcattctagttgtggtttgtccaaact
catcaatgtatcttatcatgtctggatcatcccgccatggtatcaacgcc
atatttctatttacagtagggacctcttcgttgtgtaggtaccgctgtat
tcctagggaaatagtagaggcaccttgaactgtctgcatcagccatatag
cccccgctgttcgacttacaaacacaggcacagtactgacaaacccatac
acctcctctgaaatacccatagttgctagggctgtctccgaactcattac
accctccaaagtcagagctgtaatttcgccatcaagggcagcgagggctt
ctccagataaaatagcttctgccgagagtcccgtaagggtagacacttca
gctaatccctcgatgaggtctactagaatagtcagtgcggctcccatttt
gaaaattcacttacttgatcagcttcagaagatggcggagggcctccaac
acagtaattttcctcccgactcttaaaatagaaaatgtcaagtcagttaa
gcaggaagtggactaactgacgcagctggccgtgcgacatcctcttttaa
ttagttgctaggcaacgccctccagagggcgtgtggttttgcaagaggaa
gcaaaagcctctccacccaggcctagaatgtttccacccaatcattacta
tgacaacagctgttttttttagtattaagcagaggccggggacccctggg
cccgcttactctggagaaaaagaagagaggcattgtagaggcttccagag
gcaacttgtcaaaacaggactgcttctatttctgtcacactgtctggccc
tgtcacaaggtccagcacctccataccccctttaataagcagtttgggaa
cgggtgcgggtcttactccgcccatcccgcccctaactccgcccagttcc
gcccattctccgccccatggctgactaattttttttatttatgcagaggc
cgaggccgcctcggcctctgagctattccagaagtagtgaggaggctttt
ttggaggcctaggcttttgcaaaaagctaattc
pCDNAII
Name: pCDNAII
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
ccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgc
cagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaa
tacgactcactatagggcgaattgggccctctagatgcatgctcgagcgg
ccgccagtgtgatggatatctgcagaattccagcacactggcggccgtta
ctagtggatccgagctcggtaccaagcttgatgcatagcttgagtattct
atagtgtcacctaaatagcttggcgtaatcatggtcatagctgtttcctg
tgtgaaattgtt
Full Sequence:
gggcgaattgggccctctagatgcatgctcgagcggccgccagtgtgatg
gatatctgcagaattccagcacactggcggccgttactagtggatccgag
ctcggtaccaagcttgatgcatagcttgagtattctatagtgtcacctaa
atagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttat
ccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagc
ctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcac
tgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatc
ggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttc
ctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtat
cagctcactcaaaggcggtaatacggttatccacagaatcaggggataac
gcaggaaagaacatgtgagcaaaaggccagcaaaagcccaggaaccgtaa
aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagc
atcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggacta
taaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgt
tccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaa
gcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtag
gtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccga
ccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagac
acgacttatcgccactggcagcagccactggtaacaggattagcagagcg
aggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacgg
ctacactagaaggacagtatttggtatctgcgctctgctgaagccagtta
ccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgct
ggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaa
aggatctcaagaagatcctttgatcttttctacggggtctgacgctcagt
ggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaagg
atcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcta
aagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtg
aggcacctatctcagcgatctgtctatttcgttcatccatagttgcctga
ctccccgtcgtgtagataactacgatacgggagcgcttaccatctggccc
cagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat
cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgca
actttatccgcctccattcagtctattaattgttgccgggaagctagagt
aagtagttcgccagttaatagtttgcgcaacgttgttggcattgctacag
gcatcgtggtgtcactctcgtcgtttggtatggcttcattcagctccggt
tcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcag
tgttatcactcatggttatggcagcactgcataattctcttactgtcatg
ccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatac
gggataatagtgtatcacatagcagaactttaaaagtgctcatcattgga
aaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatc
cagttcgatgtaacccactcgtgcacccaactgatcttcagcatctttta
ctttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgca
aaaaagggaataagggcgacacggaaatgttgaatactcatactcttcct
ttttcaatattattgaagcatttatcagggttattgtctcatgagcggat
acatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca
tttccccgaaaagtgccacctgtatgcggtgtgaaataccgcacagatgc
gtaaggagaaaataccgcatcaggcgaaattgtaaacgttaatattttgt
taaaattcgcgttaaatatttgttaaatcagctcattttttaaccaatag
gccgaaatcggcaaaatcccttataaatcaaaagaatagaccgcgatagg
gttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgg
actccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccacta
cgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagc
tctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaa
agccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggc
gctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacc
cgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggc
tgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgc
cagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgcc
agggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaat
acgactcactata
pCI-neo (updated)
Name: pCI-neo (updated)
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: McArdle Laboratory for Cancer Research
Vector type: plasmid
Has Custom Primer: yes
Sequencing Primers: t3, custom t7
Polylinker Sequence:
tgcagaagttggtcgtgaggcactgggcaggtaagtatcaaggttacaag
acaggtttaaggagaccaatagaaactgggcttgtcgagacagagaagac
tcttgcgtttctgataggcacctattggtcttactgacatgtccactccc
agttcaattacagctcttaaggctagagtacttaatacgactcactatag
gctagcggcgcgccgaattcgtcgacttaattaagcggccgcttcccttt
agtgagggttaatgcttcgagcagacatgataagatacattgatgagttt
ggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaat
ttgtgatgctattgctttatttgtaaccattataagctgcaataaacaag
ttaacaacaacaattgcattcattttatgtttcaggttcagggggagatg
tgggaggttttttaaagcaagt
pCMV-SPORT
Name: pCMV-SPORT
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Comments: An MluI site is introduced upon ligation of a cDNA insert.
Sequencing Primers: t7, sp6
Polylinker Sequence:
taatacgactcactatagggagagagctatgacgtcgcatgcacgcgtaa
gcttgggcccctcgagggatcctctagagcggccgc{3' - cDNA
insert -
5'}cggACGCGTgggtcgacccgggaattccggaccggtacctgcaggcg
taccttctatagtgtcacctaaatagctttttgcaaaagcctaggctaga
gtccggagg{CMV Promoter}
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcaggatcgatccagacatgataagatacattga
tgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttattt
gtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaat
aaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggg
ggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggta
tggctgattatgatcatgaacagactgtgaggactgaggggcctgaaatg
agccttgggactgtgaatctaaaatacacaaacaattagaatcactagct
cctgtgtataatattttcataaatcatactcagtaagcaaaactctcaag
cagcaagcatatgcagctagtttaacacattatacacttaaaaattttat
atttaccttagagctttaaatctctgtaggtagtttgtccaattatgtca
caccacagaagtaaggttccttcacaaagatcccaagctagcttataata
cgactcactatagggagagagctatgacgtcgcatgcacgcgtaagcttg
ggcccctcgagggatcctctagagcggccgccgactagtgagctcgtcga
cccgggaattccggaccggtacctgcaggcgtaccttctatagtgtcacc
taaatagctttttgcaaaagcctaggctagagtccggaggctggatcggt
cccggtgtcttctatggaggtcaaaacagcgtggatggcgtctccaggcg
atctgacggttcactaaacgagctctgcttatatagacctcccaccgtac
acgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttg
gaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatg
gggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccatt
gatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgt
agatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataa
tgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttgg
catatgatacacttgatgtactgccaagtgggcagtttaccgtaaatact
ccacccattgacgtcaatggaaagtccctattggcgttactatgggaaca
tacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccagg
cgggccatttaccgtaagttatgtaacgacctgcacgatgctgtttcctg
tgtgaaattgttatccgctcacaattccacacattatacgagccggaagc
tataaagtgtaaagcctggggtgcctaatgagtgaaagggcctcgtatac
gcctatttttataggttaatgtcatgataataatggtttcttagacgtca
ggtggcacttttcggggaaatgtgcgcggaacccctatttgtttattttt
ctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaa
tgcttcaataatattgaaaaacgcgcgaattgcaagctctgcattaatga
atcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgc
ttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg
tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggat
aacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccg
taaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacg
agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga
ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcc
tgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg
gaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtg
taggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcc
cgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaa
gacacgacttatcgccactggcagcagccactggtaacaggattagcaga
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaacta
cggctacactagaaggacagtatttggtatctgcgctctgctgaagccag
ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccacc
gctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaa
aaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgccataacttcgt
atagcatacattatacgaagttatggcatgagattatcaaaaaggatctt
cacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagta
tatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccc
cgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg
ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca
ataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagta
gttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatc
gtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttccca
acgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtta
gctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgtta
tcactcatggttatggcagcactgcataattctcttactgtcatgccatc
cgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgag
aatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat
aataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacg
ttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagtt
cgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttc
accagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaa
gggaataagggcgacacggaaatgttgaatactcatactcttcctttttc
aatattattgaagcatttatcagggttattgtctcatgccaggggtgggc
acacatatttgataccagcgatccctacacagcacataattcaatgcgac
ttccctctatcgcacatcttagacctttattctccctccagcacacatcg
aagctgccgagcaagccgttctcaccagtccaagacctggcatgagcgga
tacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcac
atttccccgaaaagtgccacctgaaattgtaaacgttaatattttgttaa
aattcgcgttaaatttttgttaaatcagctcattttttaaccaataggcc
gaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggtt
gagtgttgttccagtttggaacaagagtccactattaaagaacgtggact
ccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgt
gaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcact
aaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagc
cggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgct
agggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgc
cgcgcttaatgcgccgctacagggcgcgtc
pCMV-SPORT2
Name: pCMV-SPORT2
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Comments: An MluI site is introduced upon ligation of a cDNA insert.
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
gacgttgtaaaacgacggccagtgcctagcttataatacgactcactata
gggagagagctatgacgtcgcatgcacgcgtaagcttgggcccctcgagg
gatcctctagagcggccgc{3' - cDNA insert -
5'}cggACGCGTgggtcgacccgggaattccggaccggtacctgcaggcg
taccttctatagtgtcacctaaatagctttttgcaaaagcctagtggtca
tagctgtttcctgtgtgaaattgttatccgctccgcggcctaggctagag
tcc{CMV Promoter}
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcaggatcgatccagacatgataagatacattga
tgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttattt
gtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaat
aaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggg
ggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggta
tggctgattatgatcatgaacagactgtgaggactgaggggcctgaaatg
agccttgggactgtgaatctaaaatacacaaacaattagaatcactagct
cctgtgtataatattttcataaatcatactcagtaagcaaaactctcaag
cagcaagcatatgcagctagtttaacacattatacacttaaaaattttat
atttaccttagagctttaaatctctgtaggtagtttgtccaattatgtca
caccacagaagtaaggttccttcacaaagatcccaagctagcagatctcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggagagagctatgacgtcgcatgcacgcgtaagcttgggccc
ctcgagggatcctctagagcggccgccgactagtgagctcgtcgacccgg
gaattccggaccggtacctgcaggcgtaccttctatagtgtcacctaaat
agctttttgcaaaagcctagtggtcatagctgtttcctgtgtgaaattgt
tatccgctccgcggcctaggctagagtccggaggctggatcggtcccggt
gtcttctatggaggtcaaaacagcgtggatggcgtctccaggcgatctga
cggttcactaaacgagctctgcttatatagacctcccaccgtacacgcct
accgcccatttgcgtcaatggggcggagttgttacgacattttggaaagt
cccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtgg
agacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgta
ctgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgt
actgccaagtaggaaagtcccataaggtcatgtactgggcataatgccag
gcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatg
atacacttgatgtactgccaagtgggcagtttaccgtaaatactccaccc
attgacgtcaatggaaagtccctattggcgttactatgggaacatacgtc
attattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggcc
atttaccgtaagttatgtaacgacctgcacgatgctgtttcctgtgtgaa
attgttatccgctcacaattccacacattatacgagccggaagctataaa
gtgtaaagcctggggtgcctaatgagtgaaagggcctcgtatacgcctat
ttttataggttaatgtcatgataataatggtttcttagacgtcaggtggc
acttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaat
acattcaaatatgtatccgctcatgagacaataaccctgataaatgcttc
aataatattgaaaaacgcgcgaattgcaagctctgcattaatgaatcggc
caacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctc
gctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag
ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgca
ggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaa
ggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatc
acaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataa
agataccaggcgtttccccctggaagctccctcgtgcgctctcctgttcc
gaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcg
tggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtc
gttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccg
ctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacg
acttatcgccactggcagcagccactggtaacaggattagcagagcgagg
tatgtaggcggtgctacagagttcttgaagtggtggcctaactacggcta
cactagaaggacagtatttggtatctgcgctctgctgaagccagttacct
tcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt
agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagg
atctcaagaagatcctttgatcttttctacggggtctgacgctcagtgga
acgaaaactcacgttaagggattttggtcatgccataacttcgtatagca
tacattatacgaagttatggcatgagattatcaaaaaggatcttcaccta
gatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatg
agtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatc
tcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgt
gtagataactacgatacgggagggcttaccatctggccccagtgctgcaa
tgataccgcgagacccacgctcaccggctccagatttatcagcaataaac
cagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgc
ctccatccagtctattaattgttgccgggaagctagagtaagtagttcgc
cagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtg
tcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatc
aaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcct
tcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactc
atggttatggcagcactgcataattctcttactgtcatgccatccgtaag
atgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagt
gtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataatacc
gcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttc
ggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgt
aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagc
gtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaat
aagggcgacacggaaatgttgaatactcatactcttcctttttcaatatt
attgaagcatttatcagggttattgtctcatgccaggggtgggcacacat
atttgataccagcgatccctacacagcacataattcaatgcgacttccct
ctatcgcacatcttagacctttattctccctccagcacacatcgaagctg
ccgagcaagccgttctcaccagtccaagacctggcatgagcggatacata
tttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcc
ccgaaaagtgccacctgaaattgtaaacgttaatattttgttaaaattcg
cgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatc
ggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgt
tgttccagtttggaacaagagtccactattaaagaacgtggactccaacg
tcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaacca
tcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcg
gaaccctaaagggagcccccgatttagagcttgacggggaaagccggcga
acgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcg
ctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgct
taatgcgccgctacagggcgcgtc
pCMV-SPORT4
Name: pCMV-SPORT4
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Comments: An MluI site is introduced upon ligation of a cDNA insert.
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
gacgttgtaaaacgacggccagtgcctagcttataatacgactcactata
gggagagagctatgacgtcgcatgcacgcgtaagcttgggcccctcgagg
gatcctctagagcggccgc{3' - cDNA insert -
5'}cggACGCGTgggtcgacccgggaattccggaccggtacctgcaggcg
taccttctatagtgtcacctaaatagctttttgcaaaagcctagtggtca
tagctgtttcctgtgtgaaattgttatccgctccgcggcctaggctagag
tcc{CMV Promoter}
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcaggatcgatccagacatgataagatacattga
tgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttattt
gtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaat
aaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggg
ggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggta
tggctgattatgatcatgaacagactgtgaggactgaggggcctgaaatg
agccttgggactgtgaatctaaaatacacaaacaattagaatcactagct
cctgtgtataatattttcataaatcatactcagtaagcaaaactctcaag
cagcaagcatatgcagctagtttaacacattatacacttaaaaattttat
atttaccttagagctttaaatctctgtaggtagtttgtccaattatgtca
caccacagaagtaaggttccttcacaaagatcccaagctagcagttttcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggagagagctatgacgtcgcatgcacgcgtaagcttgggccc
ctcgagggatcctctagagcggccgccgactagtgagctcgtcgacccgg
gaattccggaccggtacctgcaggcgtaccttctatagtgtcacctaaat
agctttttgcaaaggcctaatggtcatagctgtttcctgtgtgaaattgt
tatccgctccgcggcctaggctagagtccggaggctggatcggtcccggt
gtcttctatggaggtcaaaacagcgtggatggcgtctccaggcgatctga
cggttcactaaacgagctctgcttatatagacctcccaccgtacacgcct
accgcccatttgcgtcaatggggcggagttgttacgacattttggaaagt
cccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtgg
agacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgta
ctgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgt
actgccaagtaggaaagtcccataaggtcatgtactgggcataatgccag
gcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatg
atacacttgatgtactgccaagtgggcagtttaccgtaaatactccaccc
attgacgtcaatggaaagtccctattggcgttactatgggaacatacgtc
attattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggcc
atttaccgtaagttatgtaacgacatgcatctaatgagtgaaagggcctc
gtactacgcctatttttataggttaatgtcatgataataatggtttctta
gacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtt
tatttttctaaatacattcaaatatgtatccgctcatgagacaataaccc
tgataaatgcttcaataatattgaaaaacgcgcgaattgcaagctctgca
ttaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct
cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgg
cgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatc
aggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcca
ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccc
cctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaaccc
gacaggactataaagataccaggcgtttccccctggaagctccctcgtgc
gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctc
ccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcag
ttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccg
ttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaac
ccggtaagacacgacttatcgccactggcagcagccactggtaacaggat
tagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggc
ctaactacggctacactagaaggacagtatttggtatctgcgctctgctg
aagccagttaccttcggaaaaagagttggtagctcttgatccggcaaaca
aaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgc
gcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtct
gacgctcagtggaacgaaaactcacgttaagggattttggtcatgccata
acttcgtatagcatacattatacgaagttatggcatgagattatcaaaaa
ggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc
taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcag
tgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct
gactccccgtcgtgtagataactacgatacgggagggcttaccatctggc
cccagtgctgcaatgataccgcgagacccacgctcaccggctccagattt
atcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctg
caactttatccgcctccatccagtctattaattgttgccgggaagctaga
gtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctac
aggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg
gttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaa
gcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgc
agtgttatcactcatggttatggcagcactgcataattctcttactgtca
tgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtca
ttctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaat
acgggataataccgcgccacatagcagaactttaaaagtgctcatcattg
gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgaga
tccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttt
tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccg
caaaaaagggaataagggcgacacggaaatgttgaatactcatactcttc
ctttttcaatattattgaagcatttatcagggttattgtctcatgccagg
ggtgggcacacatatttgataccagcgatccctacacagcacataattca
atgcgacttccctctatcgcacatcttagacctttattctccctccagca
cacatcgaagctgccgagcaagccgttctcaccagtccaagacctggcat
gagcggatacatatttgaatgtatttagaaaaataaacaaataggggttc
cgcgcacatttccccgaaaagtgccacctgaaattgtaaacgttaatatt
ttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaacca
ataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgaga
tagggttgagtgttgttccagtttggaacaagagtccactattaaagaac
gtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggccc
actacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgta
aagcactaaatcggaaccctaaagggagcccccgatttagagcttgacgg
ggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagc
gggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccacca
cacccgccgcgcttaatgcgccgctacagggcgcgtc
pCMV-SPORT6
Name: pCMV-SPORT6
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Comments: An MluI site is introduced upon ligation of a cDNA insert.
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
gatcccaagctagcagttttcccagtcacgacgttgtaaaacgacggcca
gtgcctagcttataatacgactcactatagggaccactttgtacaagaaa
gctgggtacgcgtaagcttgggcccctcgagggatactctagagcggccg
ccgactagtgagctcgtcgacgatatcccgggaattccggaccggtacca
gcctgcttttttgtacaaacttgttctatagtgtcacctaaataggccta
atggtcatagctgtttcctgtgtgaaatt
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcaggatcgatccagacatgataagatacattga
tgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttattt
gtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaat
aaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggg
ggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggta
tggctgattatgatcatgaacagactgtgaggactgaggggcctgaaatg
agccttgggactgtgaatctaaaatacacaaacaattagaatcactagct
cctgtgtataatattttcataaatcatactcagtaagcaaaactctcaag
cagcaagcatatgcagctagtttaacacattatacacttaaaaattttat
atttaccttagagctttaaatctctgtaggtagtttgtccaattatgtca
caccacagaagtaaggttccttcacaaagatcccaagctagcagttttcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggaccactttgtacaagaaagctgggtacgcgtaagcttggg
cccctcgagggatactctagagcggccgccgactagtgagctcgtcgacg
atatcccgggaattccggaccggtaccagcctgcttttttgtacaaactt
gttctatagtgtcacctaaataggcctaatggtcatagctgtttcctgtg
tgaaattgttatccgctccgcggcctaggctagagtccggaggctggatc
ggtcccggtgtcttctatggaggtcaaaacagcgtggatggcgtctccag
gcgatctgacggttcactaaacgagctctgcttatatagacctcccaccg
tacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacatt
ttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtca
atggggtggagacttggaaatccccgtgagtcaaaccgctatccacgccc
attgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaata
cgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggca
taatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtact
tggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaat
actccacccattgacgtcaatggaaagtccctattggcgttactatggga
acatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagcc
aggcgggccatttaccgtaagttatgtaacgacatgcatctaatgagtga
aagggcctcgtactacgcctatttttataggttaatgtcatgataataat
ggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaaccc
ctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaga
caataaccctgataaatgcttcaataatattgaaaaacgcgcgaattgca
agctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgta
ttgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt
cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc
cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagc
aaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagg
ctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg
gcgaaacccgacaggactataaagataccaggcgtttccccctggaagct
ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc
gcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtag
gtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacg
aaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt
gagtccaacccggtaagacacgacttatcgccactggcagcagccactgg
taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttga
agtggtggcctaactacggctacactagaaggacagtatttggtatctgc
gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagc
agattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttct
acggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt
catgccataacttcgtatagcatacattatacgaagttatggcatgagat
tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttt
aaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatg
cttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcca
tagttgcctgactccccgtcgtgtagataactacgatacgggagggctta
ccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggc
tccagatttatcagcaataaaccagccagccggaagggccgagcgcagaa
gtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg
gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgc
cattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcat
tcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttg
tgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaa
gttggccgcagtgttatcactcatggttatggcagcactgcataattctc
ttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactca
accaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgccc
ggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgc
tcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccg
ctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttc
agcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggc
aaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactc
atactcttcctttttcaatattattgaagcatttatcagggttattgtct
catgccaggggtgggcacacatatttgataccagcgatccctacacagca
cataattcaatgcgacttccctctatcgcacatcttagacctttattctc
cctccagcacacatcgaagctgccgagcaagccgttctcaccagtccaag
acctggcatgagcggatacatatttgaatgtatttagaaaaataaacaaa
taggggttccgcgcacatttccccgaaaagtgccacctgaaattgtaaac
gttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatt
ttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaat
agaccgagatagggttgagtgttgttccagtttggaacaagagtccacta
ttaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcaggg
cgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcga
ggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttaga
gcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagc
gaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcg
taaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtc
pCMV-SPORT6.1
Name: pCMV-SPORT6.1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Comments: also known as pCMV-SPORT6.1.ccdb
Sequencing Primers: M13(-21), M13 reverse, sp6
Polylinker Sequence:
caccacagaagtaaggttccttcacaaagatcccaagctagcagttttcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggaccactttgtacaagaaagctgggtacgcgtaagcttggg
cccctcgagggatactctagagcggccgccgatccggcttactaaaagcc
agataacagtatgcgtatttgcgcgctgatttttgcggtataagaatata
tactgatatgtatacccgaagtatgtcaaaaagaggtgtgcttctagaat
gcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttg
tggatgtacagagtgatattattgacacgcccgggcgacggatggtgatc
cccctggccagtgcacgtctgctgtcagataaagtctcccgtgaacttta
cccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgata
tggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagc
caccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaat
ataagtcgacgatatcccgggaattccggaccggtaccagcctgcttttt
tgtacaaacttgttctatagtgtcacctaaataggcctaatggtcatagc
tgtttcctgtgtgaaattgttatccgctccgcggcctaggctagagtccg
gaggctggatcggtcccggtgtcttctatggaggtcaaaacag
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcaggatcgatccagacatgataagatacattga
tgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttattt
gtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaat
aaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggg
ggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggta
tggctgattatgatcatgaacagactgtgaggactgaggggcctgaaatg
agccttgggactgtgaatctaaaatacacaaacaattagaatcactagct
cctgtgtataatattttcataaatcatactcagtaagcaaaactctcaag
cagcaagcatatgcagctagtttaacacattatacacttaaaaattttat
atttaccttagagctttaaatctctgtaggtagtttgtccaattatgtca
caccacagaagtaaggttccttcacaaagatcccaagctagcagttttcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggaccactttgtacaagaaagctgggtacgcgtaagcttggg
cccctcgagggatactctagagcggccgccgatccggcttactaaaagcc
agataacagtatgcgtatttgcgcgctgatttttgcggtataagaatata
tactgatatgtatacccgaagtatgtcaaaaagaggtgtgcttctagaat
gcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttg
tggatgtacagagtgatattattgacacgcccgggcgacggatggtgatc
cccctggccagtgcacgtctgctgtcagataaagtctcccgtgaacttta
cccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgata
tggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagc
caccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaat
ataagtcgacgatatcccgggaattccggaccggtaccagcctgcttttt
tgtacaaacttgttctatagtgtcacctaaataggcctaatggtcatagc
tgtttcctgtgtgaaattgttatccgctccgcggcctaggctagagtccg
gaggctggatcggtcccggtgtcttctatggaggtcaaaacagcgtggat
ggcgtctccaggcgatctgacggttcactaaacgagctctgcttatatag
acctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagtt
gttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcc
cattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgc
tatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagc
gatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtca
tgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaata
gggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagt
ttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcg
ttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttg
ggcggtcagccaggcgggccatttaccgtaagttatgtaacgacatgcat
ctaatgagtgaaagggcctcgtactacgcctatttttataggttaatgtc
atgataataatggtttcttagacgtcaggtggcacttttcggggaaatgt
gcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatc
cgctcatgagacaataaccctgataaatgcttcaataatattgaaaaacg
cgcgaattgcaagctctgcattaatgaatcggccaacgcgcggggagagg
cggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgc
gctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggta
atacggttatccacagaatcaggggataacgcaggaaagaacatgtgagc
aaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgt
ttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctca
agtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcc
ccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccg
gatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctggg
ctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggta
actatcgtcttgagtccaacccggtaagacacgacttatcgccactggca
gcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctac
agagttcttgaagtggtggcctaactacggctacactagaaggacagtat
ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggt
agctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgt
ttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctt
tgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaa
gggattttggtcatgagattatcaaaaaggatcttcacctagatcctttt
aaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaactt
ggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatc
tgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataac
tacgatacgggagggcttaccatctggccccagtgctgcaatgataccgc
gagacccacgctcaccggctccagatttatcagcaataaaccagccagcc
ggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcca
gtctattaattgttgccgggaagctagagtaagtagttcgccagttaata
gtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcg
tcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagt
tacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctc
cgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatg
gcagcactgcataattctcttactgtcatgccatccgtaagatgcttttc
tgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggc
gaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacat
agcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaa
actctcaaggatcttaccgctgttgagatccagttcgatgtaacccactc
gtgcacccaactgatcttcagcatcttttactttcaccagcgtttctggg
tgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgac
acggaaatgttgaatactcatactcttcctttttcaatattattgaagca
tttatcagggttattgtctcatgagcggatacatatttgaatgtatttag
aaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacc
tgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtc
pCMV-SPORT6.ccdb
Name: pCMV-SPORT6.ccdb
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Comments: for microquantity libraries
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
cactatagggaccactttgtacaagaaagctgggtacgcgtaagcttggg
cccctcgagggatcctctagagcggccgccgatccggcttactaaaagcc
agataacagtatgcgtatttgcgcgctgatttttgcggtataagaatata
tactgatatgtatacccgaagtatgtcaaaaagaggtgtgcttctagaat
gcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttg
tggatgtacagagtgatattattgacacgcccgggcgacggatggtgatc
cccctggccagtgcacgtctgctgtcagataaagtctcccgtgaacttta
cccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgata
tggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagc
caccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaat
ataagtcgacgatatcccgggaattccggaccggtaccagcctgcttttt
tgtacaaacttgttctatagtgtcacctaaataggcctaatggtcatagc
tgtttcctgtgtgaaattgttatccgctccgcggcctaggctagagtccg
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcaggatcgatccagacatgataagatacattga
tgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttattt
gtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaat
aaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggg
ggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggta
tggctgattatgatcatgaacagactgtgaggactgaggggcctgaaatg
agccttgggactgtgaatctaaaatacacaaacaattagaatcactagct
cctgtgtataatattttcataaatcatactcagtaagcaaaactctcaag
cagcaagcatatgcagctagtttaacacattatacacttaaaaattttat
atttaccttagagctttaaatctctgtaggtagtttgtccaattatgtca
caccacagaagtaaggttccttcacaaagatcccaagctagcagttttcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggaccactttgtacaagaaagctgggtacgcgtaagcttggg
cccctcgagggatactctagagcggccgccgactagtgagctcgtcgacg
atatcccgggaattccggaccggtaccagcctgcttttttgtacaaactt
gttctatagtgtcacctaaataggcctaatggtcatagctgtttcctgtg
tgaaattgttatccgctccgcggcctaggctagagtccggaggctggatc
ggtcccggtgtcttctatggaggtcaaaacagcgtggatggcgtctccag
gcgatctgacggttcactaaacgagctctgcttatatagacctcccaccg
tacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacatt
ttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtca
atggggtggagacttggaaatccccgtgagtcaaaccgctatccacgccc
attgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaata
cgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggca
taatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtact
tggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaat
actccacccattgacgtcaatggaaagtccctattggcgttactatggga
acatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagcc
aggcgggccatttaccgtaagttatgtaacgacatgcatctaatgagtga
aagggcctcgtactacgcctatttttataggttaatgtcatgataataat
ggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaaccc
ctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaga
caataaccctgataaatgcttcaataatattgaaaaacgcgcgaattgca
agctctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgta
ttgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt
cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc
cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagc
aaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagg
ctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg
gcgaaacccgacaggactataaagataccaggcgtttccccctggaagct
ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc
gcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtag
gtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacg
aaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt
gagtccaacccggtaagacacgacttatcgccactggcagcagccactgg
taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttga
agtggtggcctaactacggctacactagaaggacagtatttggtatctgc
gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagc
agattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttct
acggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt
catgccataacttcgtatagcatacattatacgaagttatggcatgagat
tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttt
aaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatg
cttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcca
tagttgcctgactccccgtcgtgtagataactacgatacgggagggctta
ccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggc
tccagatttatcagcaataaaccagccagccggaagggccgagcgcagaa
gtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg
gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgc
cattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcat
tcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttg
tgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaa
gttggccgcagtgttatcactcatggttatggcagcactgcataattctc
ttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactca
accaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgccc
ggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgc
tcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccg
ctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttc
agcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggc
aaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactc
atactcttcctttttcaatattattgaagcatttatcagggttattgtct
catgccaggggtgggcacacatatttgataccagcgatccctacacagca
cataattcaatgcgacttccctctatcgcacatcttagacctttattctc
cctccagcacacatcgaagctgccgagcaagccgttctcaccagtccaag
acctggcatgagcggatacatatttgaatgtatttagaaaaataaacaaa
taggggttccgcgcacatttccccgaaaagtgccacctgaaattgtaaac
gttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatt
ttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaat
agaccgagatagggttgagtgttgttccagtttggaacaagagtccacta
ttaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcaggg
cgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcga
ggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttaga
gcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagc
gaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcg
taaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtc
pCR-BluntII-TOPO
Name: pCR-BluntII-TOPO
Antibiotic: kanamycin
Concentration: 10ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Comments: PCR cloning vector, cloning site between the EcoRI sites (capitalized) as follows: GAATTCgccctt--cDNA INSERT--agggcGAATTC.
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
acagctatgaccatgattacgccaagctatttaggtgacactatagaata
ctcaagctatgcatcaagcttggtaccgagctcggatccactagtaacgg
ccgccagtgtgctggaattcgcccttaagggcgaattctgcagatatcca
tcacactggcggccgctcgagcatgcatctagagggcccaattcgcccta
tagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgact
gggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccct
ttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccca
acagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaa
gagagagccgttatcgtctgtttgtggatgtacagagtgatattattgac
acgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtc
agataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaa
gctggcgcat
Full Sequence:
agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagctcactcattaggcaccccaggctttacact
ttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacaggaaacagctatgaccatgattacgccaagctatttaggtgaca
ctatagaatactcaagctatgcatcaagcttggtaccgagctcggatcca
ctagtaacggccgccagtgtgctggaattcgcccttaagggcgaattctg
cagatatccatcacactggcggccgctcgagcatgcatctagagggccca
attcgccctatagtgagtcgtattacaattcactggccgtcgttttacaa
cgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagc
acatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatc
gcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttac
acctataaaagagagagccgttatcgtctgtttgtggatgtacagagtga
tattattgacacgccggggcgacggatggtgatccccctggccagtgcac
gtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatc
ggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggt
ctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgaca
tcaaaaacgccattaacctgatgttctggggaatataaatgtcaggcatg
agattatcaaaaaggatcttcacctagatccttttcacgtagaaagccag
tccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatct
ggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtggg
cttacatggcgatagctagactgggcggttttatggacagcaagcgaacc
ggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaag
taaactggatggctttctcgccgccaaggatctgatggcgcaggggatca
agctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaaga
tggattgcacgcaggttctccggccgcttgggtggagaggctattcggct
atgactgggcacaacagacaatcggctgctctgatgccgccgtgttccgg
ctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccgg
tgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggcca
cgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggga
agggactggctgctattgggcgaagtgccggggcaggatctcctgtcatc
tcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggc
ggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaa
catcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatca
ggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcg
ccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccat
ggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctgg
attcatcgactgtggccggctgggtgtggcggaccgctatcaggacatag
cgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgac
cgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgc
cttctatcgccttcttgacgagttcttctgaattattaacgcttacaatt
tcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgc
atacaggtggcacttttcggggaaatgtgcgcggaacccctatttgttta
tttttctaaatacattcaaatatgtatccgctcatgagacaataaccctg
ataaatgcttcaataatagcacgtgaggagggccaccatggccaagttga
ccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgag
ttctggaccgaccggctcgggttctcccgggacttcgtggaggacgactt
cgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccagg
accaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctg
gacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccggga
cgcctccgggccggccatgaccgagatcggcgagcagccgtgggggcggg
agttcgccctgcgcgacccggccggcaactgcgtgcacttcgtggccgag
gagcaggactgacacgtgctaaaacttcatttttaatttaaaaggatcta
ggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagt
tttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttct
tgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacc
accgctaccagcggtggtttgtttgccggatcaagagctaccaactcttt
ttccgaaggtaactggcttcagcagagcgcagataccaaatactgtcctt
ctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcc
tacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcg
ataagtcgtgtcttaccgggttggactcaagacgatagttaccggataag
gcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgga
gcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaa
gcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggc
agggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctg
gtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgat
ttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaac
gcggcctttttacggttcctgggcttttgctggccttttgctcacatgtt
ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttg
agtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtca
gtgagcgaggaagcggaag
pCR-XL-TOPO
Name: pCR-XL-TOPO
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Comments: this vector has both kanamycin and zeocin resistance
Sequencing Primers: M13(-21), M13 reverse, t7
Polylinker Sequence:
CACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACG
CGTTAGAATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCA
CTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTAAGGGCGAATTCTG
CAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCA
ATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAA
CGTCGTGACTGGGAAAACCCTGGCGT
Full Sequence:
AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAA
TGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAA
CGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACT
TTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTT
CACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACG
CGTTAGAATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCA
CTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTAAGGGCGAATTCTG
CAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCA
ATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAA
CGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGC
ACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATC
GCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTTTAAGGTTTAC
ACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGA
TATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCAC
GTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATC
GGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGT
CTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACA
TCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATG
AGATTATCAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAG
TCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCT
GGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGG
CTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACC
GGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAG
TAAACTGGATGGCTTTCTCGCCGCCAAGGATCTGATGGCGCAGGGGATCA
AGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGA
TGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCT
ATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGG
CTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGG
TGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCA
CGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGA
AGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATC
TCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGC
GGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAA
CATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCA
GGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCG
CCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCAT
GGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGG
ATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAG
CGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGAC
CGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGC
CTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAACGCTTACAATT
TCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGC
ATACAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTG
ATAAATGCTTCAATAATAGCACGTGAGGAGGGCCACCATGGCCAAGTTGA
CCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAG
TTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTT
CGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGG
ACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTG
GACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGA
CGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGG
AGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAG
GAGCAGGACTGACACGTGCTAAAACTTCATTTTTAATTTAAAAGGATCTA
GGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGT
TTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCT
TGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACC
ACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTT
TTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTT
CTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCC
TACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCG
ATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAG
GCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGA
GCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAA
GCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGC
AGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTG
GTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAT
TTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAC
GCGGCCTTTTTACGGTTCCTGGGCTTTTGCTGGCCTTTTGCTCACATGTT
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG
AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA
GTGAGCGAGGAAGCGGAAG
pCR2.1-TOPO
Name: pCR2.1-TOPO
Antibiotic: ampicillin
Concentration: 100 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Comments: PCR cloning vector, cloning site between the EcoRI sites (capitalized) as follows: GAATTCgccctt--cDNA INSERT--agggcGAATTC.
Sequencing Primers: M13(-21), M13 reverse, t7
Polylinker Sequence:
cacacaggaaacagctatgaccatgattacgccaagcttggtaccgagct
cggatccactagtaacggccgccagtgtgctggaattcgcccttaagggc
gaattctgcagatatccatcacactggcggccgctcgagcatgcatctag
agggcccaattcgccctatagtgagtcgtattacaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg
caccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacg
Full Sequence:
agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagctcactcattaggcaccccaggctttacact
ttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacaggaaacagctatgaccatgattacgccaagcttggtaccgagct
cggatccactagtaacggccgccagtgtgctggaattcgcccttaagggc
gaattctgcagatatccatcacactggcggccgctcgagcatgcatctag
agggcccaattcgccctatagtgagtcgtattacaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg
caccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacg
cgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagc
gtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttctt
cccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatc
gggggctccctttagggttccgatttagagctttacggcacctcgaccgc
aaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgata
gacggtttttcgccctttgacgttggagtccacgttctttaatagtggac
tcttgttccaaactggaacaacactcaaccctatcgcggtctattctttt
gatttataagggattttgccgatttcggcctattggttaaaaaatgagct
gatttaacaaattcagggcgcaagggctgctaaaggaaccggaacacgta
gaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctact
gggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagct
tgcagtgggcttacatggcgatagctagactgggcggttttatggacagc
aagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagc
cctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgc
aggggatcaagatctgatcaagagacaggatgaggatcgtttcgcatgat
tgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggc
tattcggctatgactgggcacaacagacaatcggctgctctgatgccgcc
gtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccga
cctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgt
ggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcact
gaagcgggaagggactggctgctattgggcgaagtgccggggcaggatct
cctgtcatctcgccttgctcctgccgagaaagtatccatcatggctgatg
caatgcggcggctgcatacgcttgatccggctacctgcccattcgaccac
caagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtct
tgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccg
aactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtc
gtgatccatggcgatgcctgcttgccgaatatcatggtggaaaatggccg
cttttctggattcaacgactgtggccggctgggtgtggcggaccgctatc
aggacatagcgttggatacccgtgatattgctgaagagcttggcggcgaa
tgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgca
gcgcatcgccttctatcgccttcttgacgagttcttctgaattgaaaaag
gaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg
cggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagta
aaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactgga
tctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttc
caatgatgagcacttttaaagttctgctatgtcatacactattatcccgt
attgacgccgggcaagagcaactcggtcgccgggcgcggtattctcagaa
tgacttggttgagtactcaccagtcacagaaaagcatcttacggatggca
tgacagtaagagaattatgcagtgctgccataaccatgagtgataacact
gcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgc
ttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaac
cggagctgaatgaagccataccaaacgacgagagtgacaccacgatgcct
gtagcaatgccaacaacgttgcgcaaactattaactggcgaactacttac
tctagcttcccggcaacaattaatagactggatggaggcggataaagttg
caggaccacttctgcgctcggcccttccggctggctggtttattgctgat
aaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggg
gccagatggtaagccctcccgtatcgtagttatctacacgacggggagtc
aggcaactatggatgaacgaaatagacagatcgctgagataggtgcctca
ctgattaagcattggtaactgtcagaccaagtttactcatatatacttta
gattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcc
tttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccac
tgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatccttt
ttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccag
cggtggtttgtttgccggatcaagagctaccaactctttttccgaaggta
actggcttcagcagagcgcagataccaaatactgtccttctagtgtagcc
gtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcg
ctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgt
cttaccgggttggactcaagacgatagttaccggataaggcgcagcggtc
gggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacct
acaccgaactgagatacctacagcgtgagcattgagaaagcgccacgctt
cccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaac
aggagagcgcacgagggagcttccagggggaaacgcctggtatctttata
gtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgc
tcgtcaggggggcggagcctatggaaaaacgccagcaacgcggccttttt
acggttcctggccttttgctggccttttgctcacatgttctttcctgcgt
tatcccctgattctgtggataaccgtattaccgcctttgagtgagctgat
accgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgagga
agcggaag
pCR3.1
Name: pCR3.1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Polylinker Sequence:
tcgaaattaatacgactcactatagggagacccaagctggctagcgttta
aacttaagcttggtaccgagctcggatccactagtccagtgtggtggaat
tcggcttaagccgaattctgcagatatccagcacagtggcggccgctcga
gtctagagggcccgtttaaacccgctgatcagcctcgactgtgccttcta
gttgccagcc
Full Sequence:
gcgcgcgttgacattgattattgactagttattaatagtaatcaattacg
gggtcattagttcatagcccatatatggagttccgcgttacataacttac
ggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgt
caataatgacgtatgttcccatagtaacgccaatagggactttccattga
cgtcaatgggtggactatttacggtaaactgcccacttggcagtacatca
agtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaat
ggcccgcctggcattatgcccagtacatgaccttatgggactttcctact
tggcagtacatctacgtattagtcatcgctattaccatggtgatgcggtt
ttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttc
caagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaat
caacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaat
gggcggtaggcgtgtacggtgggaggtctatataagcagagctctctggc
taactagagaacccactgcttactggcttatcgaaattaatacgactcac
tatagggagacccaagctggctagcgtttaaacttaagcttggtaccgag
ctcggatccactagtccagtgtggtggaattcggcttaagccgaattctg
cagatatccagcacagtggcggccgctcgagtctagagggcccgtttaaa
cccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtt
tgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgt
cctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtc
attctattctggggggtggggtggggcaggacagcaagggggaggattgg
gaagacaatagcaggcatgctggggatgcggtgggctctatggcttctga
ggcggaaagaaccagtggcggtaatacggttatccacagaatcaggggat
aacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccg
taaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacg
agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga
ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcc
tgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg
gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtg
taggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcc
cgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaa
gacacgacttatcgccactggcagcagccactggtaacaggattagcaga
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaacta
cggctacactagaaggacagtatttggtatctgcgctctgctgaagccag
ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccacc
gctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaa
aaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa
aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaat
ctaaagtatatatgagtaacctgaggctatggcagggcctgccgccccga
cgttggctgcgagccctgggccttcacccgaacttggggggtggggtggg
gaaaaggaagaaacgcgggcgtattggccccaatggggtctcggtggggt
atcgacagagtgccagccctgggaccgaaccccgcgtttatgaacaaacg
acccaacaccgtgcgttttattctgtctttttattgccgtcatagcgcgg
gttccttccggtattgtctccttccgtgtttcagttagcctccccctagg
gtgggcgaagaactccagcatgagatccccgcgctggaggatcatccagc
cggcgtcccggaaaacgattccgaagcccaacctttcatagaaggcggcg
gtggaatcgaaatctcgtgatggcaggttgggcgtcgcttggtcggtcat
ttcgaaccccagagtcccgctcagaagaactcgtcaagaaggcgatagaa
ggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagc
ggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaac
gctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaa
tccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgc
catgggtcacgacgagatcctcgccgtcgggcatgctcgccttgagcctg
gcgaacagttcggctggcgcgagcccctgatgctcttgatcatcctgatc
gacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcg
cttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgc
attgcatcagccatgatggatactttctcggcaggagcaaggtgagatga
caggagatcctgccccggcacttcgcccaatagcagccagtcccttcccg
cttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggcc
agccacgatagccgcgctgcctcgtcttgcagttcattcagggcaccgga
caggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccgga
acacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccg
aatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttg
ttcaatcatgcgaaacgatcctcatcctgtctcttgatcgatctttgcaa
aagcctaggcctccaaaaaagcctcctcactacttctggaatagctcaga
ggccgaggcggcctcggcctctgcataaataaaaaaaattagtcagccat
ggggcggagaatgggcggaactgggcggagttaggggcgggatgggcgga
gttaggggcgggactatggttgctgactaattgagatgcatgctttgcat
acttctgcctgctggggagcctggggactttccacacctggttgctgact
aattgagatgcatgctttgcatacttctgcctgctggggagcctggggac
tttccacaccctaactgacacacattccacagctggttctttccgcctca
ggactcttcctttttcaataaatcaatctaaagtatatatgagtaaactt
ggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatc
tgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataac
tacgatacgggagggcttaccatctggccccagtgctgcaatgataccgc
gagacccacgctcaccggctccagatttatcagcaataaaccagccagcc
ggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcca
gtctattaattgttgccgggaagctagagtaagtagttcgccagttaata
gtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcg
tcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagt
tacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctc
cgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatg
gcagcactgcataattctcttactgtcatgccatccgtaagatgcttttc
tgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggc
gaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacat
agcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaa
actctcaaggatcttaccgctgttgagatccagttcgatgtaacccactc
gtgcacccaactgatcttcagcatcttttactttcaccagcgtttctggg
tgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgac
acggaaatgttgaatactcatactcttcctttttcaatattattgaagca
tttatcagggttattgtctcatgagcggatacatatttgaatgtatttag
aaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacc
tgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgc
gcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgct
ttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctct
aaatcgggggctccctttagggttccgatttagtgctttacggcacctcg
accccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccc
tgatagacggtttttcgccctttgacgttggagtccacgttctttaatag
tggactcttgttccaaactggaacaacactcaaccctatctcggtctatt
cttttgatttataagggattttgccgatttcggcctattggttaaaaaat
gagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgct
tacaatttac
pCR4-TOPO
Name: pCR4-TOPO
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Comments: this vector has both ampicillin and kanamycin-resistance
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
GCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCAC
GACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGT
GAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGG
CTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAAC
AGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGA
CTAGTCCTGCAGGTTTAAACGAATTCGCCCTTAAGGGCGAATTCGCGGCC
GCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGT
CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATC
GCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCC
CGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTT
TAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATG
TACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTG
GCCAGTGCACGTCTGCTG
Full Sequence:
AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAA
TGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAA
CGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACT
TTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTT
CACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAACCC
TCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTAAGGGC
GAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAA
TTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTA
CCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAAT
AGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATA
CGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTC
TGTTTGTGGATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATG
GTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGA
ACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCA
CCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGAT
CTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTG
GGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATG
AATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAG
AAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGG
TTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGT
AAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAG
GATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGAT
CGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCT
TGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTG
CTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTT
TTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCA
GCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCT
CGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGC
CGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCC
ATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTG
CCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGA
TGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGG
CTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGG
CGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGG
TGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTG
GCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGA
GCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCG
CTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTC
TGAATTATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCA
TCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCC
GCTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAA
AATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGAC
AGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATT
TCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATAC
GGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCA
CGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGC
CGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTA
ATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGC
AACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGG
TATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGAT
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT
GTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACT
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG
GTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGT
TGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAAC
TTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAA
GGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCC
AACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAA
AACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAAT
GTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAG
GGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTG
AGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTT
TTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCG
GTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAC
TGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGT
AGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCT
CTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCT
TACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGG
GCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTAC
ACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC
CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG
GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGT
CCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTC
GTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAC
GGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTA
TCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATAC
CGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAG
CGGAAG
pCR4Blunt-TOPO
Name: pCR4Blunt-TOPO
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Comments: This vector has both kanamycin and ampicillin resistance
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagctcactcattaggcaccccaggctttacact
ttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacaggaaacagctatgaccatgattacgccaagctcagaattaaccc
tcactaaagggactagtcctgcaggtttaaacgaattcgcccttaagggc
gaattcgcggccgctaaattcaattcgccctatagtgagtcgtattacaa
ttcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta
cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaat
agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctata
cgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtc
tgtttgtggatgtacagagtgatattattgacacgccggggcgacggatg
gtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtga
actttacccggtggtgcatatcggggatgaaagctggcgcatgatgacca
ccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgat
ctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctg
gggaatataaatgtcaggcatgagattatcaaaaaggatcttcacctaga
tccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatg
aatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagag
aaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcgg
ttttatggacagcaagcgaaccggaattgccagctggggcgccctctggt
aaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaag
gatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggat
cgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgct
tgggtggagaggctattcggctatgactgggcacaacagacaatcggctg
ctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttcttt
ttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggca
gcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgct
cgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgc
cggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatcc
atcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctg
cccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcgga
tggaagccggtcttgtcgatcaggatgatctggacgaagagcatcagggg
ctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacgg
cgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatgg
tggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtg
gcggaccgctatcaggacatagcgttggctacccgtgatattgctgaaga
gcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccg
ctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttc
tgaattattaacgcttacaatttcctgatgcggtattttctccttacgca
tctgtgcggtatttcacaccgcatcaggtggcacttttcggggaaatgtg
cgcggaacccctatttgtttatttttctaaatacattcaaatatgtatcc
gctcatgagattatcaaaaaggatcttcacctagatccttttaaattaaa
aatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgac
agttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatt
tcgttcatccatagttgcctgactccccgtcgtgtagataactacgatac
gggagggcttaccatctggccccagtgctgcaatgataccgcgagaccca
cgctcaccggctccagatttatcagcaataaaccagccagccggaagggc
cgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatta
attgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgc
aacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttgg
tatggcttcattcagctccggttcccaacgatcaaggcgagttacatgat
cccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgtt
gtcagaagtaagttggccgcagtgttatcactcatggttatggcagcact
gcataattctcttactgtcatgccatccgtaagatgcttttctgtgactg
gtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagt
tgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaac
tttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaa
ggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcaccc
aactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaa
aacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaat
gttgaatactcatactcttcctttttcaatattattgaagcatttatcag
ggttattgtctcatgaccaaaatcccttaacgtgagttttcgttccactg
agcgtcagaccccgtagaaaagatcaaaggatcttcttgagatccttttt
ttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcg
gtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaac
tggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgt
agttaggccaccacttcaagaactctgtagcaccgcctacatacctcgct
ctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtct
taccgggttggactcaagacgatagttaccggataaggcgcagcggtcgg
gctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctac
accgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcc
cgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacag
gagagcgcacgagggagcttccagggggaaacgcctggtatctttatagt
cctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctc
gtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttac
ggttcctggccttttgctggccttttgctcacatgttctttcctgcgtta
tcccctgattctgtggataaccgtattaccgcctttgagtgagctgatac
cgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaag
cggaag
pCS105
Name: pCS105
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: UCBerkeley/Harland
Vector type: plasmid
Has Custom Primer: yes
Vector Map: Available Here
Comments: vector size 4.1 kb
Polylinker Sequence:
cgccattctgcctggggacgtcggagcaagcttgatttaggtgacactat
agaatacaagctacttgttctttttgcaggatcccatcgattcgaattcg
tcgacaggccaatctggccgcggccgcaaggcctctcgagcctctagaac
tatagtgagtcgtattacgtagatccagacatgataagatacattgatga
gtttggacaaaccacaactagaatgcagtgaaaaaaatgc
Full Sequence:
cgccattctgcctggggacgtcggagcaagcttgatttaggtgacactat
agaatacaagctacttgttctttttgcaggatcccatcgattcgaattcg
tcgacaggccaatctggccgcggccgcaaggcctctcgagcctctagaac
tatagtgagtcgtattacgtagatccagacatgataagatacattgatga
gtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtg
aaatttgtgatgctattgctttatttgtaaccattataagctgcaataaa
caagttaacaacaacaattgcattcattttatgtttcaggttcaggggga
ggtgtgggaggttttttaattcgcggcgcgccgcggcgccaatgcattgg
gcccggtacccagcttttgttccctttagtgagggttaattgcgcgcttg
gcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcac
aattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgct
ttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg
cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctca
ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac
tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg
cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg
ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg
ccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta
tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga
aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa
aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgt
cgtgtagataactacgatacgggagggcttaccatctggccccagtgctg
caatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc
cgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt
cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg
atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct
ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatca
ctcatggttatggcagcactgcataattctcttactgtcatgccatccgt
aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat
accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga
tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg
aataagggcgacacggaaatgttgaatactcatactcttcctttttcaat
attattgaagcatttatcagggttattgtctcatgagcggatacatattt
gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg
aaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgtt
aaatttttgttaaatcagctcattttttaaccaataggccgaaatcggca
aaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgtt
ccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaa
agggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcac
cctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaac
cctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgt
ggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctgg
caagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaat
gcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttg
ggaagggcgatcggtgcgggcctcttcgctattacgccagtcgatcgacc
atagccaattcaatatggcgtatatggactcatgccaattcaatatggtg
gatctggacctgtgccaattcaatatggcgtatatggactcgtgccaatt
caatatggtggatctggaccccagccaattcaatatggcggacttggcac
catgccaattcaatatggcggacttggcactgtgccaactggggaggggt
ctacttggcacggtgccaagtttgaggaggggtcttggccctgtgccaag
tccgccatattgaattggcatggtgccaataatggcggccatattggcta
tatgccaggatcaatatataggcaatatccaatatggccctatgccaata
tggctattggccaggttcaatactatgtattggccctatgccatatagta
ttccatatatgggttttcctattgacgtagatagcccctcccaatgggcg
gtcccatataccatatatggggcttcctaataccgcccatagccactccc
ccattgacgtcaatggtctctatatatggtctttcctattgacgtcatat
gggcggtcctattgacgtatatggcgcctcccccattgacgtcaattacg
gtaaatggcccgcctggctcaatgcccattgacgtcaataggaccaccca
ccattgacgtcaatgggatggctcattgcccattcatatccgttctcacg
ccccctattgacgtcaatgacggtaaatggcccacttggcagtacatcaa
tatctattaatagtaacttggcaagtacattactattggcaagtacgcca
agggtacattggcagtactcccattgacgtcaatggcggtaaatggcccg
cgatggctgccaagtacatccccattgacgtcaatggggaggggcaatga
cgcaaatgggcgttccattgacgtaaatgggcggtaggcgtgcctaatgg
gaggtctatataagcaatgctcgtttagggaac
pCS107
Name: pCS107
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: UCBerkeley/Harland
Vector type: plasmid
Has Custom Primer: yes
Vector Map: Available Here
Comments: vector size 4.1 kb
Sequencing Primers: t7, sp6
Polylinker Sequence:
cgccattctgcctggggacgtcggagcaagcttgatttaggtgacactat
agaatacaagctacttgttctttttgcaggatcccatcgattcgaattcg
tcgacaggccaatctggccgcggccgcaaggcctctcgagcctctgcggc
tatagtgagtcgtattacgtagatccagacatgataagatacattgatga
gtttggacaaaccacaactagaatgcagtgaaaaaaatgc
Full Sequence:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGACACTAT
AGAATACAAGCTACTTGTTCTTTTTGCAGGATCCCATCGATTCGAATTCG
TCGACAGGCCAATCTGGCCGCGGCCGCAAGGCCTCTCGAGCCTCTGCGGC
TATAGTGAGTCGTATTACGTAGATCCAGACATGATAAGATACATTGATGA
GTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTG
AAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGA
GGTGTGGGAGGTTTTTTAATTCGCGGCCGCGGCGCCAATGCATTGGGCCC
GGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGT
AATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATT
CCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTA
ATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCC
AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG
GGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGA
CTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAAC
ATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT
GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAG
GCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCC
GCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC
AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT
ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGC
GGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAG
GACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGT
TTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGA
AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAG
ATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGA
GTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCT
CAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTG
TAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAAT
GATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACC
AGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCC
TCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCC
AGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGT
CACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCA
AGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTT
CGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCA
TGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGA
TGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTG
TATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCG
CGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCG
GGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTA
ACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCG
TTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATA
AGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTA
TTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAAT
GTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAA
GTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAAT
TTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAAT
CCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAG
TTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGG
CGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTA
ATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTA
AAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCG
AGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAG
TGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGC
CGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAA
GGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGTCGACCATAGCCAA
TTCAATATGGCGTATATGGACTCATGCCAATTCAATATGGTGGATCTGGA
CCTGTGCCAATTCAATATGGCGTATATGGACTCGTGCCAATTCAATATGG
TGGATCTGGACCCCAGCCAATTCAATATGGCGGACTTGGCACCATGCCAA
TTCAATATGGCGGACTTGGCACTGTGCCAACTGGGGAGGGGTCTACTTGG
CACGGTGCCAAGTTTGAGGAGGGGTCTTGGCCCTGTGCCAAGTCCGCCAT
ATTGAATTGGCATGGTGCCAATAATGGCGGCCATATTGGCTATATGCCAG
GATCAATATATAGGCAATATCCAATATGGCCCTATGCCAATATGGCTATT
GGCCAGGTTCAATACTATGTATTGGCCCTATGCCATATAGTATTCCATAT
ATGGGTTTTCCTATTGACGTAGATAGCCCCTCCCAATGGGCGGTCCCATA
TACCATATATGGGGCTTCCTAATACCGCCCATAGCCACTCCCCCATTGAC
GTCAATGGTCTCTATATATGGTCTTTCCTATTGACGTCATATGGGCGGTC
CTATTGACGTATATGGCGCCTCCCCCATTGACGTCAATTACGGTAAATGG
CCCGCCTGGCTCAATGCCCATTGACGTCAATAGGACCACCCACCATTGAC
GTCAATGGGATGGCTCATTGCCCATTCATATCCGTTCTCACGCCCCCTAT
TGACGTCAATGACGGTAAATGGCCCACTTGGCAGTACATCAATATCTATT
AATAGTAACTTGGCAAGTACATTACTATTGGAAGGACGCCAGGGTACATT
GGCAGTACTCCCATTGACGTCAATGGCGGTAAATGGCCCGCGATGGCTGC
CAAGTACATCCCCATTGACGTCAATGGGGAGGGGCAATGACGCAAATGGG
CGTTCCATTGACGTAAATGGGCGGTAGGCGTGCCTAATGGGAGGTCTATA
TAAGCAATGCTCGTTTAGGGAAC
pCS108
Name: pCS108
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: UCBerkeley/Harland
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: t7, t3, sp6
Polylinker Sequence:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGACACTAT
AGAATACAAGCTACTTGTTCTTTTTGCAGGATCCCATCGATTCGAATTCG
TCGACAGGCCAATCTGGCCGCGGCCGCAAGGCCTGATATCTCTAGACCGC
GGCTCGAGCCTCTCGCCCTATAGTGAGTCGTATTACGTAGATCCAGACAT
GATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA
AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTAT
GTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCGCGCC
GCGGCGCCAATGCATTGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGA
GGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTG
AAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAA
AGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCG
Full Sequence:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGACACTAT
AGAATACAAGCTACTTGTTCTTTTTGCAGGATCCCATCGATTCGAATTCG
TCGACAGGCCAATCTGGCCGCGGCCGCAAGGCCTGATATCTCTAGACCGC
GGCTCGAGCCTCTCGCCCTATAGTGAGTCGTATTACGTAGATCCAGACAT
GATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA
AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTAT
GTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCGCGCC
GCGGCGCCAATGCATTGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGA
GGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTG
AAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAA
AGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCG
TTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA
TTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCT
CTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGG
CGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC
AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCC
CCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCC
GACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTC
CCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAG
TTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAAC
CCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGAT
TAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTG
AAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA
AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCT
GACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATT
ATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTA
AATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGC
TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCAT
AGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTAC
CATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCT
CCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAG
TGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGG
AAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCC
ATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATT
CAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGT
GCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAG
TTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCT
TACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAA
CCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCG
GCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCT
CATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGC
TGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCA
GCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCA
AAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCA
TACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTC
ATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGT
TCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATAT
TTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACC
AATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAG
ATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAA
CGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCC
CACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGT
AAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACG
GGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAG
CGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACC
ACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATT
CAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TACGCCAGTCGATCGACCATAGCCAATTCAATATGGCGTATATGGACTCA
TGCCAATTCAATATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTA
TATGGACTCGTGCCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCA
ATATGGCGGACTTGGCACCATGCCAATTCAATATGGCGGACTTGGCACTG
TGCCAACTGGGGAGGGGTCTACTTGGCACGGTGCCAAGTTTGAGGAGGGG
TCTTGGCCCTGTGCCAAGTCCGCCATATTGAATTGGCATGGTGCCAATAA
TGGGGCCATATTGGCTATATGCCAGGATCAATATATAGGCAATATCCAAT
ATGGCCCTATGCCAATATGGCTATTGGCCAGGTTCAATACTATGTATTGG
CCCTATGCCATATAGTATTCCATATATGGGTTTTCCTATTGACGTAGATA
GCCCCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTTCCTAATAC
CGCCCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATATATGGTCTT
TCCTATTGACGTCATATGGGCGGTCCTATTGACGTATATGGCGCCTCCCC
CATTGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGCCCATTGAC
GTCAATAGGACCACCCACCATTGACGTCAATGGGATGGCTCATTGCCCAT
TCATATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTAAATGGCCC
ACTTGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAGTACATTAC
TATTGGCAAGTACGCCAAGGGTACATTGGCAGTACTCCCATTGACGTCAA
TGGCGGTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTGACGTCA
ATGGGGAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAATGGGCG
GTAGGCGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTAGGGAAC
pCS111
Name: pCS111
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: UCBerkeley/Harland
Vector type: plasmid
Vector Map: Available Here
Comments: Mustafa Khokha modified pCS108 by cloning a linker between the EcoRI and NotI sites eliminating the SfiI site and creating a SmaI site. This creates a vector suitable for cloning cDNAs using the methods from the XGC.
Sequencing Primers: t7, t3, sp6
Polylinker Sequence:
TGTGCCAATTCAATATGGCGTATATGGACTCGTGCCAATTCAATATGGTG
GATCTGGACCCCAGCCAATTCAATATGGCTATTGGCCAGGTTCAATACTA
TGTATTGGCCCTATGCCATATAGTATTCCATATATGGGTTTTCCTATTGA
CGTAGATAGCCCCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTT
CCTAATACCGCCCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATAT
ATGGTCTTTCCTATTGACGTCATATGGGCGGTCCTATTGACGTATATGGC
GCCTCCCCCATTGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGC
CCATTGACGTCAATAGGACCACCCACCATTGACGTCAATGGGATGGCTCA
TTGCCCATTCATATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTA
AATGGCCCACTTGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAG
TACATTACTATTGGAAGTACGCCAGGGTACATTGGCAGTACTCCCATTGA
CGTCAATGGCGGTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTG
ACGTCAATGGGGAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAA
TGGGCGGTAGGCGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTA
GGGAACCGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGA
CACTATAGAATACAAGCTACTTGTTCTTTTTGCAGGATCCCATCGATTCG
AATTCGTCCCGGGAATCTGGCCGCGGCCGCAAGGCCTGATATCTCTAGAC
CGCGGCTCGAGCCTCTCGCCCTATAGTGAGTCGTATTACGTAGATCCAGA
CATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGT
GAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTT
TATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCGC
GCCGCGGCGCCAATGCATTGGGCCCGGTACCCAGCTTTTGTTCCCTTTAG
TGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGT
GTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGGAGCA
TAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATT
GCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
GCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGC
GCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTG
CGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGA
TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGG
pCS2+
Name: pCS2+
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Max Planck Institute/Rupp
Vector type: plasmid
Has Custom Primer: yes
Vector Map: Available Here
Comments: Expression vector, T7 may be mutated. NOTES: Vector size 4095 bp. pCS2+ is an expression vector primarily designed for expressing proteins in Xenopus embryos from either injected RNA or DNA, pCS2+ is also useful for high-level transient expression in a wide variety of mammalian and avian cells. It is also functional in zebrafish embryos (as DNA or RNA) and it can be used for invitro transcription/translation. pCS2+ contains a strong enhancer/promoter (simian CMV 1E94) followed by a polylinker and the SV40 late polyadenylation site. An sp6 promoter is present in the 5' UTR, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site provide several sites to linearize the vector for Sp6 RNA transcription. Vector backbone is pBluescript II KS+ and includes the amp gene and an f1 origin for single stranded DNA.
Sequencing Primers: t7, sp6
Polylinker Sequence:
cgccattctgcctggggacgtcggagcaagcttgatttaggtgacactat
agaatacaagctacttgttctttttgcaggatcccatcgattcgaattca
aggcctctcgagcctctagaactatagtgagtcgtattacgtagatcgag
a
Full Sequence:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGACACTAT
AGAATACAAGCTACTTGTTCTTTTTGCAGGATCCCATCGATTCGAATTCA
AGGCCTCTCGAGCCTCTAGAACTATAGTGAGTCGTATTACGTAGATCCAG
ACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGT
AACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATT
TTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCC
GCGGCGCCAATGCATTGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGA
GGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTG
AAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAA
AGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCG
TTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA
TTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCT
CTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGG
CGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC
AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCC
CCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCC
GACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTC
CCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAG
TTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAAC
CCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGAT
TAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTG
AAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA
AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCT
GACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATT
ATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTA
AATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGC
TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCAT
AGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTAC
CATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCT
CCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAG
TGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGG
AAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCC
ATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATT
CAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGT
GCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAG
TTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCT
TACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAA
CCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCG
GCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCT
CATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGC
TGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCA
GCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCA
AAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCA
TACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTC
ATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGT
TCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATAT
TTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACC
AATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAG
ATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAA
CGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCC
CACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGT
AAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACG
GGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAG
CGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACC
ACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATT
CAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TACGCCAGTCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGCC
AATTCAATATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATG
GACTCGTGCCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATAT
GGCGGACTTGGCACCATGCCAATTCAATATGGCGGACTTGGCACTGTGCC
AACTGGGGAGGGGTCTACTTGGCACGGTGCCAAGTTTGAGGAGGGGTCTT
GGCCCTGTGCCAAGTCCGCCATATTGAATTGGCATGGTGCCAATAATGGC
GGCCATATTGGCTATATGCCAGGATCAATATATAGGCAATATCCAATATG
GCCCTATGCCAATATGGCTATTGGCCAGGTTCAATACTATGTATTGGCCC
TATGCCATATAGTATTCCATATATGGGTTTTCCTATTGACGTAGATAGCC
CCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTTCCTAATACCGC
CCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATATATGGTCTTTCC
TATTGACGTCATATGGGCGGTCCTATTGACGTATATGGCGCCTCCCCCAT
TGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGCCCATTGACGTC
AATAGGACCACCCACCATTGACGTCAATGGGATGGCTCATTGCCCATTCA
TATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCACT
TGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAGTACATTACTAT
TGGAAGGACGCCAGGGTACATTGGCAGTACTCCCATTGACGTCAATGGCG
GTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTGACGTCAATGGG
GAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAATGGGCGGTAGG
CGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTAGGGAAC
pCS22+
Name: pCS22+
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: UCBerkeley/Harland
Vector type: plasmid
Vector Map: Available Here
Polylinker Sequence:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTTAATACGACTCACTATA
GGGCAATACAAGCTACTTGTTTAAACTCTGCAGGGATCCGAATTCCTCGA
GCCCTTTAGTGAGGGTTAATTTACGTAGATCCAGACATGATAAGATACAT
TGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTA
TTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCA
GGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCCGCGGCGCCAATGCATT
GGGCCCGGTACCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGT
GAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATA
AAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGC
GTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGC
ATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC
TCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCG
GCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCC
AGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCC
CCCTGACGAGCATCACAAAA
Full Sequence:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTTAATACGACTCACTATA
GGGCAATACAAGCTACTTGTTTAAACTCTGCAGGGATCCGAATTCCTCGA
GCCCTTTAGTGAGGGTTAATTTACGTAGATCCAGACATGATAAGATACAT
TGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTA
TTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCA
GGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCCGCGGCGCCAATGCATT
GGGCCCGGTACCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGT
GAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATA
AAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGC
GTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGC
ATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC
TCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCG
GCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCC
AGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCC
CCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTG
CGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCT
CCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCC
GTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAA
CCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGG
CCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCT
GAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACG
CGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTC
TGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTT
AAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATG
CTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCA
TAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTA
CCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGC
TCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAA
GTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGG
GAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGC
CATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCAT
TCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTG
TGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAA
GTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTC
TTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCA
ACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCC
GGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGC
TCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCG
CTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTC
AGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGC
AAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTC
ATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCT
CATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGG
TTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATA
TTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAAC
CAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGA
GATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGA
ACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGC
CCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCG
TAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGAC
GGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGA
GCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCAC
CACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCAT
TCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTA
TTACGCCAGTCGACCATAGCCAATTCAATATGGCGTATATGGACTCATGC
CAATTCAATATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATAT
GGACTCGTGCCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATA
TGGCGGACTTGGCACCATGCCAATTCAATATGGCGGACTTGGCACTGTGC
CAACTGGGGAGGGGTCTACTTGGCACGGTGCCAAGTTTGAGGAGGGGTCT
TGGCCCTGTGCCAAGTCCGCCATATTGAATTGGCATGGTGCCAATAATGG
CGGCCATATTGGCTATATGCCAGGATCAATATATAGGCAATATCCAATAT
GGCCCTATGCCAATATGGCTATTGGCCAGGTTCAATACTATGTATTGGCC
CTATGCCATATAGTATTCCATATATGGGTTTTCCTATTGACGTAGATAGC
CCCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTTCCTAATACCG
CCCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATATATGGTCTTTC
CTATTGACGTCATATGGGCGGTCCTATTGACGTATATGGCGCCTCCCCCA
TTGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGCCCATTGACGT
CAATAGGACCACCCACCATTGACGTCAATGGGATGGCTCATTGCCCATTC
ATATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCAC
TTGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAGTACATTACTA
TTGGAAGGACGCCAGGGTACATTGGCAGTACTCCCATTGACGTCAATGGC
GGTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTGACGTCAATGG
GGAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAATGGGCGGTAG
GCGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTAGGGAAC
pCS2G
Name: pCS2G
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: RIKEN/Carninci
Vector type: plasmid
Has Custom Primer: yes
Vector Map: Available Here
Comments: pCS2-based expression vector
Sequencing Primers: t7, sp6
Polylinker Sequence:
aagcttgatttaggtgacactatagaatacaagctacttgttctttttgc
aggatctctcgaatcacaagtttgtacaaaaaagcaggctctcgagctct
aataaggtgacactatagaacca--------5'-cDNA_insert-3'--
----ctctccagtattggatccacccagctttcttgtacaaagtggtgat
gatccgtccgtcgagcctctacattctgcagccctatagtgagtcgtatt
acgtaga
Full Sequence:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGACACTAT
AGAATACAAGCTACTTGTTCTTTTTGCAGGATCTCTCGAATCACAAGTTT
GTACAAAAAAGCAGGCTCTCGAGCTCTAATAAGGTGACACTATAGAACCA
NNNCTCTCCAGTATTGGATCCACCCAGCTTTCTTGTACAAAGTGGTGATG
ATCCGTCCGTCGAGCCTCTACATTCTGCAGCCCTATAGTGAGTCGTATTA
CGTAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAA
CTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAA
TTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTT
AATTCGCGGCCGCGGCGCCAATGCATTGGGCCCGGTACCCAGCTTTTGTT
CCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTG
TTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGC
CGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCA
CATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCG
TGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG
TATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCG
TTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTA
TCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCA
GCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGG
TGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAG
CTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGT
AGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCA
CGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT
GGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTT
GAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGA
TCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCA
GCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTG
GTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAA
ATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACA
GTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTT
CGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACG
GGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCAC
GCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCC
GAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAA
TTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCA
ACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGT
ATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATC
CCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTG
TCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTG
CATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGG
TGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTT
GCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACT
TTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAG
GATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCA
ACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAA
ACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATG
TTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGG
GTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAA
CAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTA
AGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTC
ATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAG
AATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCA
CTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCA
GGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGT
CGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTT
AGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAA
AGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGC
GCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCC
CATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGC
CTCTTCGCTATTACGCCAGTCGACCATAGCCAATTCAATATGGCGTATAT
GGACTCATGCCAATTCAATATGGTGGATCTGGACCTGTGCCAATTCAATA
TGGCGTATATGGACTCGTGCCAATTCAATATGGTGGATCTGGACCCCAGC
CAATTCAATATGGCGGACTTGGCACCATGCCAATTCAATATGGCGGACTT
GGCACTGTGCCAACTGGGGAGGGGTCTACTTGGCACGGTGCCAAGTTTGA
GGAGGGGTCTTGGCCCTGTGCCAAGTCCGCCATATTGAATTGGCATGGTG
CCAATAATGGCGGCCATATTGGCTATATGCCAGGATCAATATATAGGCAA
TATCCAATATGGCCCTATGCCAATATGGCTATTGGCCAGGTTCAATACTA
TGTATTGGCCCTATGCCATATAGTATTCCATATATGGGTTTTCCTATTGA
CGTAGATAGCCCCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTT
CCTAATACCGCCCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATAT
ATGGTCTTTCCTATTGACGTCATATGGGCGGTCCTATTGACGTATATGGC
GCCTCCCCCATTGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGC
CCATTGACGTCAATAGGACCACCCACCATTGACGTCAATGGGATGGCTCA
TTGCCCATTCATATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTA
AATGGCCCACTTGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAG
TACATTACTATTGGAAGGACGCCAGGGTACATTGGCAGTACTCCCATTGA
CGTCAATGGCGGTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTG
ACGTCAATGGGGAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAA
TGGGCGGTAGGCGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTA
GGGAAC
pDeliver1.1
Name: pDeliver1.1
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: GeneCopoeia
Vector type: Gateway entry vector
pDNR-Dual
Name: pDNR-Dual
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Clontech
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13(-21), t7
Polylinker Sequence:
gcggccgcataacttcgtatagcatacattatacgaagttatcagtcgac
ggtaccggacatatgcccgggaattcctgcaggatccgctcgagaagctt
tctagaccattcgtttggcgcgcgggcccaggtgagtggtcataatcata
atcataatcataatcataatcacaactagcctaggagatcctggtcatga
ctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgt
tctgaacaaatccagatggagttctgaggtcattactggatctatc
Full Sequence:
gcggccgcataacttcgtatagcatacattatacgaagttatcagtcgac
ggtaccggacatatgcccgggaattcctgcaggatccgctcgagaagctt
tctagaccattcgtttggcgcgcgggcccaggtgagtggtcataatcata
atcataatcataatcataatcacaactagcctaggagatcctggtcatga
ctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgt
tctgaacaaatccagatggagttctgaggtcattactggatctatcaaca
ggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatc
gcagtactgttgtaattcattaagcattctgccgacatggaagccatcac
aaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgcct
tgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccat
attggccacgtttaaatcaaaactggtgaaactcacccagggattggctg
agacgaaaaacatattctcaataaaccctttagggaaataggccaggttt
tcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaa
atcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcat
ggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccg
tctttcattgccatacgaaattccggatgagcattcatcaggcgggcaag
aatgtgaataaaggccggataaaacttgtgcttatttttctttacggtct
ttaaaaaggccgtaatatccagctgaacggtctggttataggtacattga
gcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatat
atcaacggtggtatatccagtgatttttttctccattttagcttccttag
ctcctgaaagatccataacttcgtatagcatacattatacgaagttatgc
ggccgcgacgtccacatatacctgccgttcactattatttagtgaaatga
gatattatgatattttctgaattgtgattaaaaaggcaactttatgccca
tgcaacagaaactataaaaaatacagagaatgaaaagaaacagatagatt
ttttagttctttaggcccgtagtctgcaaatccttttatgattttctatc
aaacaaaagaggaaaatagaccagttgcaatccaaacgagagtctaatag
aatgaggtcgaaaagtaaatcgcgcgggtttgttactgataaagcaggca
agacctaaaatgtgtaaagggcaaagtgtatactttggcgtcacccctta
catattttaggtctttttttattgtgcgtaactaacttgccatcttcaaa
caggagggctggaagaagcagaccgctaacacagtacataaaaaaggaga
catgaacgatgaacatcaaaaagtttgcaaaacaagcaacagtattaacc
tttactaccgcactgctggcaggaggcgcaactcaagcgtttgcgaaaga
aacgaaccaaaagccatataaggaaacatacggcatttcccatattacac
gccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaa
gttcctgagttcgattcgtccacaattaaaaatatctcttctgcaaaagg
cctggacgtttgggacagctggccattacaaaacgctgacggcactgtcg
caaactatcacggctaccacatcgtctttgcattagccggagatcctaaa
aatgcggatgacacatcgatttacatgttctatcaaaaagtcggcgaaac
ttctattgacagctggaaaaacgctggccgcgtctttaaagacagcgaca
aattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtca
ggttcagccacatttacatctgacggaaaaatccgtttattctacactga
tttctccggtaaacattacggcaaacaaacactgacaactgcacaagtta
acgtatcagcatcagacagctctttgaacatcaacggtgtagaggattat
aaatcaatctttgacggtgacggaaaaacgtatcaaaatgtacagcagtt
catcgatgaaggcaactacagctcaggcgacaaccatacgctgagagatc
ctcactacgtagaagataaaggccacaaatacttagtatttgaagcaaac
actggaactgaagatggctaccaaggcgaagaatctttatttaacaaagc
atactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttc
tgcaaagcgataaaaaacgcacggctgagttagcaaacggcgctctcggt
atgattgagctaaacgatgattacacactgaaaaaagtgatgaaaccgct
gattgcatctaacacagtaacagatgaaattgaacgcgcgaacgtcttta
aaatgaacggcaaatggtacctgttcactgactcccgcggatcaaaaatg
acgattgacggcattacgtctaacgatatttacatgcttggttatgtttc
taattctttaactggcccatacaagccgctgaacaaaactggccttgtgt
taaaaatggatcttgatcctaacgatgtaacctttacttactcacacttc
gctgtacctcaagcgaaaggaaacaatgtcgtgattacaagctatatgac
aaacagaggattctacgcagacaaacaatcaacgtttgcgcctagcttcc
tgctgaacatcaaaggcaagaaaacatctgttgtcaaagacagcatcctt
gaacaaggacaattaacagttaacaaataaaaacgcaaaagaaaatgccg
atatcctattggcattgacgtcaggtggcacttttcggggaaatgtgcgc
ggaacccctatttgtttatttttctaaatacattcaaatatgtatccgct
catgagacaataaccctgataaatgcttcaataatattgaaaaaggaaga
gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca
ttttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaaga
tgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctca
acagcggtaagatccttgagagttttcgccccgaagaacgttttccaatg
atgagcacttttaaagttctgctatgtggcgcggtattatcccgtattga
cgccgggcaagagcaactcggtcgccgcatacactattctcagaatgact
tggttgagtactcaccagtcacagaaaagcatcttacggatggcatgaca
gtaagagaattatgcagtgctgccataaccatgagtgataacactgcggc
caacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttt
tgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggag
ctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagc
aatggcaacaacgttgcgcaaactattaactggcgaactacttactctag
cttcccggcaacaattaatagactggatggaggcggataaagttgcagga
ccacttctgcgctcggcccttccggctggctggtttattgctgataaatc
tggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccag
atggtaagccctcccgtatcgtagttatctacacgacggggagtcaggca
actatggatgaacgaaatagacagatcgctgagataggtgcctcactgat
taagcattggtaactgtcagaccaagtttactcatatatactttagattg
atttaaaacttcatttttaatttaaaaggatctaggtgaagatccttttt
gataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagc
gtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttc
tgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtg
gtttgtttgccggatcaagagctaccaactctttttccgaaggtaactgg
cttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagt
taggccaccacttcaagaactctgtagcaccgcctacatacctcgctctg
ctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttac
cgggttggactcaagacgatagttaccggataaggcgcagcggtcgggct
gaacggggggttcgtgcacacagcccagcttggagcgaacgacctacacc
gaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccga
agggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggag
agcgcacgagggagcttccagggggaaacgcctggtatctttatagtcct
gtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtc
aggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggt
tcctggccttttgctggccttttgctcacatgttctttcctgcgttatcc
cctgattctgtggataaccgtattaccgccttacgcgtgtaaaacgacgg
ccagtagatctgtaatacgactcactatagggcgctagctgctcgccgca
gccgaacgaccgagcgcagcgagtcagtgagcgaggaa
pDNR-LIB
Name: pDNR-LIB
Antibiotic: chloramphenicol
Concentration: 25 ug/ml
Source: Clontech
Vector type: plasmid
Vector Map: Available Here
Polylinker Sequence:
gcggccgcataacttcgtatagcatacattatacgaagttatcagtcgac
ggtaccggacatatgcccgggaattcggccattacggcctgcaggatccg
aaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaa
ttgttgttgttaacttgtttattgcagcttataatggttacaaataaagc
aatagcatcacaaatttcacaaataaagcatttttttcactgcattctag
ttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccggc
cgcctcggccctcgagaagctttctagaccattcgtttggcgcgcgggcc
cagtaggtaagtgaacatggtcatagctgtttccctaggagatcctggta
tgactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgag
cgttctgaacaaatccagatggagttctgaggtcattactggatctatca
Full Sequence:
gcggccgcataacttcgtatagcatacattatacgaagttatcagtcgac
ggtaccggacatatgcccgggaattcggccattacggcctgcaggatccg
aaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaa
ttgttgttgttaacttgtttattgcagcttataatggttacaaataaagc
aatagcatcacaaatttcacaaataaagcatttttttcactgcattctag
ttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccggc
cgcctcggccctcgagaagctttctagaccattcgtttggcgcgcgggcc
cagtaggtaagtgaacatggtcatagctgtttccctaggagatcctggta
tgactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgag
cgttctgaacaaatccagatggagttctgaggtcattactggatctatca
acaggagtccaagcgagctcgatatcaaattacgccccgccctgccactc
atcgcagtactgttgtaattcattaagcattctgccgacatggaagccat
cacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcg
ccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtc
catattggccacgtttaaatcaaaactggtgaaactcacccagggattgg
ctgagacgaaaaacatattctcaataaaccctttagggaaataggccagg
ttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccg
gaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgct
catggaaaacggtgtaacaagggtgaacactatcccatatcaccagctca
ccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggc
aagaatgtgaataaaggccggataaaacttgtgcttatttttctttacgg
tctttaaaaaggccgtaatatccagctgaacggtctggttataggtacat
tgagcaactgactgaaatgcctcaaaatgttctttacgatgccattggga
tatatcaacggtggtatatccagtgatttttttctccattttagcttcct
tagctcctgaaagatccataacttcgtatagcatacattatacgaagtta
tgcggccgcgacgtcaatgccaataggatatcggcattttcttttgcgtt
tttatttgttaactgttaattgtccttgttcaaggatgctgtctttgaca
acagatgttttcttgcctttgatgttcagcaggaagctaggcgcaaacgt
tgattgtttgtctgcgtagaatcctctgtttgtcatatagcttgtaatca
cgacattgtttcctttcgcttgaggtacagcgaagtgtgagtaagtaaag
gttacatcgttaggatcaagatccatttttaacacaaggccagttttgtt
cagcggcttgtatgggccagttaaagaattagaaacataaccaagcatgt
aaatatcgttagacgtaatgccgtcaatcgtcatttttgatccgcgggag
tcagtgaacaggtaccatttgccgttcattttaaagacgttcgcgcgttc
aatttcatctgttactgtgttagatgcaatcagcggtttcatcacttttt
tcagtgtgtaatcatcgtttagctcaatcataccgagagcgccgtttgct
aactcagccgtgcgttttttatcgctttgcagaagtttttgactttcttg
acggaagaatgatgtgcttttgccatagtatgctttgttaaataaagatt
cttcgccttggtagccatcttcagttccagtgtttgcttcaaatactaag
tatttgtggcctttatcttctacgtagtgaggatctctcagcgtatggtt
gtcgcctgagctgtagttgccttcatcgatgaactgctgtacattttgat
acgtttttccgtcaccgtcaaagattgatttataatcctctacaccgttg
atgttcaaagagctgtctgatgctgatacgttaacttgtgcagttgtcag
tgtttgtttgccgtaatgtttaccggagaaatcagtgtagaataaacgga
tttttccgtcagatgtaaatgtggctgaacctgaccattcttgtgtttgg
tcttttaggatagaatcatttgcatcgaatttgtcgctgtctttaaagac
gcggccagcgtttttccagctgtcaatagaagtttcgccgactttttgat
agaacatgtaaatcgatgtgtcatccgcatttttaggatctccggctaat
gcaaagacgatgtggtagccgtgatagtttgcgacagtgccgtcagcgtt
ttgtaatggccagctgtcccaaacgtccaggccttttgcagaagagatat
ttttaattgtggacgaatcgaactcaggaacttgattttttcattttttt
gctgttcagggatttgcagcatatcatggcgtgtaatatgggaaatgccg
tatgtttccttatatggcttttggttcgtttctttcgcaaacgcttgagt
tgcgcctcctgccagcagtgcggtagtaaaggttaatactgttgcttgtt
ttgcaaactttttgatgttcatcgttcatgtctccttttttatgtactgt
gttagcggtctgcttcttccagccctcctgtttgaagatggcaagttagt
tacgcacaataaaaaaagacctaaaatatgtaaggggtgacgccaaagta
tacactttgccctttacacattttaggtcttgcctgctttatcagtaaca
aacccgcgcgatttacttttcgacctcattctattagactctcgtttgga
ttgcaactggtctattttcctcttttgtttgatagaaaatcataaaagga
tttgcagactacgggcctaaagaactaaaaaatctatctgtttcttttca
ttctctgtattttttatagtttctgttgcatgggcataaagttgcctttt
taatcacaattcagaaaatatcataatatctcatttcactaaataatagt
gaacggcaggtatatgtggcgcgcctaagcattggtaactgtcagaccaa
gtttactcatatatactttagattgatttaaaacttcatttttaatttaa
aaggatctaggtgaagatcctttttgataatctcatgaccaaaatccctt
aacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaa
ggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaac
aaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctac
caactctttttccgaaggtaactggcttcagcagagcgcagataccaaat
actgttcttctagtgtagccgtagttaggccaccacttcaagaactctgt
agcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctg
ccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtta
ccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcc
cagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagc
tatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccg
gtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggg
aaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttg
agcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaac
gccagcaacgcggcctttttacggttcctggccttttgctggccttttgc
tcacatgttctttcctgcgttatcccctgattctgtggataaccgtatta
ccgccttacgcgtgtaaaacgacggccagtagatctgtaatacgactcac
tatagggcgctagctcgccgcagccgaacgaccgagcgcagcgagtcagt
gagcgaggaa
pDNR-NCI
Name: pDNR-NCI
Antibiotic: chloramphenicol
Concentration: 25 ug/ml
Source: Clontech
Vector type: phagemid
Vector Map: Available Here
Comments: Clontech preliminary MGC vector
Polylinker Sequence:
GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTCGAC
GAATTCTGGATCTGGAAACAGCTATGACCATGCTCGGGAAGCGCGCCATT
GTGTTGGTACCCGGGAATTCGGCCATTATGGCCTGCAGGATCCGGTGGTG
GTGCAAATCAAAGAACTGCTCCTCAGTGGATGTTGCCTTTACTTCTAGGC
CTGTACGGAAGTGTTACTTCTGCTCTAAAAGCTGCGGAATTGTACCCGCG
GGCCCACCATGGCATCAATGCAGAAGCTGATCTCAGAGGAGGACCTGCTT
ATGGCCATGGAGGCCCGAATTCGGTCGACCGAGATCTCTCGAGGTACCGC
GGCCGCGGGGATCCGGCCGCCTCGGCCCAGTCGACTCTAGACTCGAGCAA
GCTTATGCATGCGGCCGCAATTCGAGCTCACTTGGCCAATTCGCCCTATA
GTGAGTCGTATTAACTGGCCGTCGTTTTACAGATCCTGGCATGACTAGTG
CTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAA
CAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTC
CAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTA
CTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGG
CATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGC
Full Sequence:
GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTCGAC
GAATTCTGGATCTGGAAACAGCTATGACCATGCTCGGGAAGCGCGCCATT
GTGTTGGTACCCGGGAATTCGGCCATTATGGCCTGCAGGATCCGGTGGTG
GTGCAAATCAAAGAACTGCTCCTCAGTGGATGTTGCCTTTACTTCTAGGC
CTGTACGGAAGTGTTACTTCTGCTCTAAAAGCTGCGGAATTGTACCCGCG
GGCCCACCATGGCATCAATGCAGAAGCTGATCTCAGAGGAGGACCTGCTT
ATGGCCATGGAGGCCCGAATTCGGTCGACCGAGATCTCTCGAGGTACCGC
GGCCGCGGGGATCCGGCCGCCTCGGCCCAGTCGACTCTAGACTCGAGCAA
GCTTATGCATGCGGCCGCAATTCGAGCTCACTTGGCCAATTCGCCCTATA
GTGAGTCGTATTAACTGGCCGTCGTTTTACAGATCCTGGCATGACTAGTG
CTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAA
CAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTC
CAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTA
CTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGG
CATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTA
TAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGC
CACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGA
AAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCG
TAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTC
GTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAA
CGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTC
ATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTG
AATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAA
AGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACT
GACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAAC
GGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTG
AAAGATCCATAACTTCGTATAGCATACATTATACGAAGTTATGCGGCCGC
GACGTCAATGCCAATAGGATATCGGCATTTTCTTTTGCGTTTTTATTTGT
TAACTGTTAATTGTCCTTGTTCAAGGATGCTGTCTTTGACAACAGATGTT
TTCTTGCCTTTGATGTTCAGCAGGAAGCTAGGCGCAAACGTTGATTGTTT
GTCTGCGTAGAATCCTCTGTTTGTCATATAGCTTGTAATCACGACATTGT
TTCCTTTCGCTTGAGGTACAGCGAAGTGTGAGTAAGTAAAGGTTACATCG
TTAGGATCAAGATCCATTTTTAACACAAGGCCAGTTTTGTTCAGCGGCTT
GTATGGGCCAGTTAAAGAATTAGAAACATAACCAAGCATGTAAATATCGT
TAGACGTAATGCCGTCAATCGTCATTTTTGATCCGCGGGAGTCAGTGAAC
AGATACCATTTGCCGTTCATTTTAAAGACGTTCGCGCGTTCAATTTCATC
TGTTACTGTGTTAGATGCAATCAGCGGTTTCATCACTTTTTTCAGTGTGT
AATCATCGTTTAGCTCAATCATACCGAGAGCGCCGTTTGCTAACTCAGCC
GTGCGTTTTTTATCGCTTTGCAGAAGTTTTTGACTTTCTTGACGGAAGAA
TGATGTGCTTTTGCCATAGTATGCTTTGTTAAATAAAGATTCTTCGCCTT
GGTAGCCATCTTCAGTTCCAGTGTTTGCTTCAAATACTAAGTATTTGTGG
CCTTTATCTTCTACGTAGTGAGGATCTCTCAGCGTATGGTTGTCGCCTGA
GCTGTAGTTGCCTTCATCGATGAACTGCTGTACATTTTGATACGTTTTTC
CGTCACCGTCAAAGATTGATTTATAATCCTCTACACCGTTGATGTTCAAA
GAGCTGTCTGATGCTGATACGTTAACTTGTGCAGTTGTCAGTGTTTGTTT
GCCGTAATGTTTACCGGAGAAATCAGTGTAGAATAAACGGATTTTTCCGT
CAGATGTAAATGTGGCTGAACCTGACCATTCTTGTGTTTGGTCTTTTAGG
ATAGAATCATTTGCATCGAATTTGTCGCTGTCTTTAAAGACGCGGCCAGC
GTTTTTCCAGCTGTCAATAGAAGTTTCGCCGACTTTTTGATAGAACATGT
AAATCGATGTGTCATCCGCATTTTTAGGATCTCCGGCTAATGCAAAGACG
ATGTGGTAGCCGTGATAGTTTGCGACAGTGCCGTCAGCGTTTTGTAATGG
CCAGCTGTCCCAAACGTCCAGGCCTTTTGCAGAAGAGATATTTTTAATTG
TGGACGAATCGAATTCAGGAACTTGATATTTTTCATTTTTTTGCTGTTCA
GGGATTTGCAGCATATCATGGCGTGTAATATGGGAAATGCCGTATGTTTC
CTTATATGGCTTTTGGTTCGTTTCTTTCGCAAACGCTTGAGTTGCGCCTC
CTGCCAGCAGTGCGGTAGTAAAGGTTAATACTGTTGCTTGTTTTGCAAAC
TTTTTGATGTTCATCGTTCATGTCTCCTTTTTTATGTACTGTGTTAGCGG
TCTGCTTCTTCCAGCCCTCCTGTTTGAAGATGGCAAGTTAGTTACGCACA
ATAAAAAAAGACCTAAAATATGTAAGGGGTGACGCCAAAGTATACACTTT
GCCCTTTACACATTTTAGGTCTTGCCTGCTTTATCAGTAACAAACCCGCG
CGATTTACTTTTCGACCTCATTCTATTAGACTCTCGTTTGGATTGCAACT
GGTCTATTTTCCTCTTTTGTTTGATAGAAAATCATAAAAGGATTTGCAGA
CTACGGGCCTAAAGAACTAAAAAATCTATCTGTTTCTTTTCATTCTCTGT
ATTTTTTATAGTTTCTGTTGCATGGGCATAAAGTTGCCTTTTTAATCACA
ATTCAGAAAATATCATAATATCTCATTTCACTAAATAATAGTGAACGGCA
GGTATATGTGGCGCGCCTAAGCATTGGTAACTGTCAGACCAAGTTTACTC
ATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCT
AGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAG
TTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTC
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC
CACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTT
TTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCT
TCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGC
CTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGC
GATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAA
GGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGG
AGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAA
AGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGG
CAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCT
GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGA
TTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA
CGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT
TCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT
GAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTC
AGTGAGCGAGGAA
pDONR201
Name: pDONR201
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Has Custom Primer: yes
Vector Map: Available Here
Polylinker Sequence:
cctactctcgcgttaacgctagcatggatctcgggccccaaataatgatt
ttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatg
cttttttataatgccaagtttgtacaaaaaagcaggctnn....cDNA
insert...nacccagctttcttgtacaaagtgggcattataagaaagca
ttgcttatcaatttgttgcaacgaacaggtcactatcagtcaaaataaaa
tcattatttgccatccagctgcagctctggcccgtgtctcaaaatctctg
atgttacattgcacaagataaaaatatatcatcatgaacaataaaactgt
ctgcttaca
Full Sequence:
ctttcctgcgttatcccctgattctgtggataaccgtattaccgctagcc
aggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttct
gcttagtttgatgcctggcagtttatggcgggcgtcctgcccgccaccct
ccgggccgttgcttcacaacgttcaaatccgctcccggcggatttgtcct
actcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagt
cttccgactgagcctttcgttttatttgatgcctggcagttccctactct
cgcgttaacgctagcatggatctcgggccccaaataatgattttattttg
actgatagtgacctgttcgttgcaacaaattgatgagcaatgctttttta
taatgccaagtttgtacaaaaaagcagaacgagaaacgtaaaatgatata
aatatcaatatattaaattagattttgcataaaaaacagactacataata
ctgtaaaacacaacatatccagtcactatgaatcaactacttagatggta
ttagtgacctgtagtcgaccgacagccttccaaatgttcttcgggtgatg
ctgccaacttagtcgaccgacagccttccaaatgttcttctcaaacggaa
tcgtcgtatccagcctactcgctattgtcctcaatgccgtattaaatcat
aaaaagaaataagaaaaagaggtgcgagcctcttttttgtgtgacaaaat
aaaaacatctacctattcatatacgctagtgtcatagtcctgaaaatcat
ctgcatcaagaacaatttcacaactcttatacttttctcttacaagtcgt
tcggcttcatctggattttcagcctctatacttactaaacgtgataaagt
ttctgtaatttctactgtatcgacctgcagactggctgtgtataagggag
cctgacatttatattccccagaacatcaggttaatggcgtttttgatgtc
attttcgcggtggctgagatcagccacttcttccccgataacggagaccg
gcacactggccatatcggtggtcatcatgcgccagctttcatccccgata
tgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgc
actggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcac
tctgtacatccacaaacagacgataacggctctctcttttataggtgtaa
accttaaactgcatttcaccagtccctgttctcgtcagcaaaagagccgt
tcatttcaataaaccgggcgacctcagccatcccttcctgattttccgct
ttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgct
taccagaccggagatattgacatcatatatgccttgagcaactgatagct
gtcgctgtcaactgtcactgtaatacgctgcttcatagcacacctctttt
tgacatacttcgggtatacatatcagtatatattcttataccgcaaaaat
cagcgcgcaaatacgcatactgttatctggcttttagtaagccggatcca
cgcgattacgccccgccctgccactcatcgcagtactgttgtaattcatt
aagcattctgccgacatggaagccatcacagacggcatgatgaacctgaa
tcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatg
gtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaa
actggtgaaactcacccagggattggctgagacgaaaaacatattctcaa
taaaccctttagggaaataggccaggttttcaccgtaacacgccacatct
tgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactcca
gagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggt
gaacactatcccatatcaccagctcaccgtctttcattgccatacggaat
tccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggata
aaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatcca
gctgaacggtctggttataggtacattgagcaactgactgaaatgcctca
aaatgttctttacgatgccattgggatatatcaacggtggtatatccagt
gatttttttctccattttagcttccttagctcctgaaaatctcgataact
caaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaa
cctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccaggg
cttcccggtatcaacagggacaccaggatttatttattctgcgaagtgat
cttccgtcacaggtatttattcggcgcaaagtgcgtcgggtgatgctgcc
aacttagtcgactacaggtcactaataccatctaagtagttgattcatag
tgactggatatgttgtgttttacagtattatgtagtctgttttttatgca
aaatctaatttaatatattgatatttatatcattttacgtttctcgttca
gctttcttgtacaaagtgggcattataagaaagcattgcttatcaatttg
ttgcaacgaacaggtcactatcagtcaaaataaaatcattatttgccatc
cagctgcagctctggcccgtgtctcaaaatctctgatgttacattgcaca
agataaaaatatatcatcatgaacaataaaactgtctgcttacataaaca
gtaatacaaggggtgttatgagccatattcaacgggaaacgtcgaggccg
cgattaaattccaacatggatgctgatttatatgggtataaatgggctcg
cgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagc
ccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaat
gatgttacagatgagatggtcagactaaactggctgacggaatttatgcc
tcttccgaccatcaagcattttatccgtactcctgatgatgcatggttac
tcaccactgcgatccccggaaaaacagcattccaggtattagaagaatat
cctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccg
gttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtat
ttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcg
agtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaa
agaaatgcataaacttttgccattctcaccggattcagtcgtcactcatg
gtgatttctcacttgataaccttatttttgacgaggggaaattaataggt
tgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgc
catcctatggaactgcctcggtgagttttctccttcattacagaaacggc
tttttcaaaaatatggtattgataatcctgatatgaataaattgcagttt
catttgatgctcgatgagtttttctaatcagaattggttaattggttgta
acactggcagagcattacgctgacttgacgggacggcgcaagctcatgac
caaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtag
aaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgc
tgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccgga
tcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgc
agataccaaatactgtccttctagtgtagccgtagttaggccaccacttc
aagaactctgtagcaccgcctacatacctcgctctgctaatcctgttacc
agtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaa
gacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcg
tgcacacagcccagcttggagcgaacgacctacaccgaactgagatacct
acagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcgg
acaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggag
cttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcca
cctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcc
tatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgc
tggccttttgctcacatgtt
pDONR223
Name: pDONR223
Antibiotic: spectinomycin
Concentration: 100 ug/ml
Source: Dana Farber Cancer Institute/Vidal Lab
Vector type: plasmid
Vector Map: Available Here
Polylinker Sequence:
AACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGAC
TGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATA
ATGCCAACTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAA
TATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACT
GTAAAACACAACATATCCAGTCACTATGAATCAACTACTTAGATGGTATT
AGTGACCTGTA-insert-CCCTATAGTGAGTCGTATTACATGGTCATAG
CTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTG
Full Sequence:
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG
AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA
GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC
GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA
AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA
GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA
GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG
CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA
GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC
GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT
TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC
AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC
CTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTT
TGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATA
TTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACA
ACATATCCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGT
AGTCGACCGACAGCCTTCCAAATGTTCTTCGGGTGATGCTGCCAACTTAG
TCGACCGACAGCCTTCCAAATGTTCTTCTCAAACGGAATCGTCGTATCCA
GCCTACTCGCTATTGTCCTCAATGCCGTATTAAATCATAAAAAGAAATAA
GAAAAAGAGGTGCGAGCCTCTTTTTTGTGTGACAAAATAAAAACATCTAC
CTATTCATATACGCTAGTGTCATAGTCCTGAAAATCATCTGCATCAAGAA
CAATTTCACAACTCTTATACTTTTCTCTTACAAGTCGTTCGGCTTCATCT
GGATTTTCAGCCTCTATACTTACTAAACGTGATAAAGTTTCTGTAATTTC
TACTGTATCGACCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTAT
ATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTG
GCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCA
TATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGG
TAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGG
GATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCA
CAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGC
ATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAA
ACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCG
GCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGA
GATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAAC
TGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCG
GGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAAT
ACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCC
CCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCC
GACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCA
TCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGG
GCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACT
CACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAG
GGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATG
TGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAA
CGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCC
ATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCA
TTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTT
ATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCT
GGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTA
CGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTC
CATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGC
CCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGC
CGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATC
AACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAG
GTATTTATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTAGTCGAC
TACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGGATATG
TTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTA
ATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTAC
AAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACA
GGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCC
CCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGG
CCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATC
ATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGC
TGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGA
ACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCG
TATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTA
ACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTA
CAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCG
ATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCC
CTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCG
ACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGAC
GTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGC
CACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAA
ACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCC
TGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACG
ACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGA
GAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGAT
CGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTG
CCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAG
GATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCC
CGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTT
GGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGAC
TGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGC
TAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAG
ATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTA
GTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAG
TTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT
CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAA
AAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAAC
TCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTG
TCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCA
CCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAG
TGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGG
ATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGC
TTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTG
AGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAA
GCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAAC
GCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCG
TCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCA
GCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC
ATGTT
pENTR/D-TOPO
Name: pENTR/D-TOPO
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: Invitrogen Life Technologies
Vector type: Gateway entry vector
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7
Polylinker Sequence:
TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC
AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC
CTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTT
TGTACAAAAAAGCAGGCTCCGCGGCCGCCCCCTTCACC-ORF-AAGGGTG
GGCGCGCCGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCAT
TGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAAT
CATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGG
TCATAGCTGTTTCCTGGCAGCTCTG
Full Sequence:
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG
AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA
GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC
GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA
AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA
GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA
GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG
CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA
GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC
GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT
TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC
AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC
CTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTT
TGTACAAAAAAGCAGGCTCCGCGGCCGCCCCCTTCACC-ORF-AAGGGTG
GGCGCGCCGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCAT
TGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAAT
CATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGG
TCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGT
TACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGC
TTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAA
CGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTAT
AAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTT
GTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTA
GCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACG
GAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGA
TGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTAT
TAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTG
TTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAG
CGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTT
TGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAA
CAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGT
CGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGA
AATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATAC
CAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATT
ACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATA
AATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTT
AATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGC
AAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAG
CGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTT
CTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGT
GGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTG
GCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAG
TTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCT
GCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTA
CCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGC
TGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACAC
CGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCG
AAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGA
GAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCC
TGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGT
CAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGG
TTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT
pENTR201
Name: pENTR201
Antibiotic: kanamycin
Concentration: 10ug/ml
Source: Invitrogen Life Technologies
Vector type: Gateway entry vector
Vector Map: Available Here
Sequencing Primers: pDONR201-forward, pDONR201-reverse
Polylinker Sequence:
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACC
GCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATG
GCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCG
CCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGAT
TTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAG
GCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCC
CTACTCTCGCGTTAACGCTAGCATGGATCTCGGGCCCCAAATAATGATTT
TATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGC
TTTTTTATAATGCCAACTTTGTACAAAAAAGCTGGCACCATG-linker-
ORF-linker-GGCGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGA
AAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAA
TAAAATCATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAAT
CTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAA
ACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTC
AACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTA
TATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAAT
CTATCGCTTGTATGGGAAGCCCGATGCGC
Full Sequence:
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACC
GCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATG
GCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCG
CCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGAT
TTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAG
GCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCC
CTACTCTCGCGTTAACGCTAGCATGGATCTCGGGCCCCAAATAATGATTT
TATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGC
TTTTTTATAATGCCAACTTTGTACAAAAAAGCTGGCACCATG-linker-
ORF-linker-GGCGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGA
AAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAA
TAAAATCATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAAT
CTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAA
ACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTC
AACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTA
TATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAAT
CTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATG
GCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAAC
TGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTAC
TCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCAT
TCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCG
CTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCC
TTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGA
ATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGG
CCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACC
GGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTG
ACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCA
GACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTC
TCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTG
ATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCA
GAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACG
GGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCA
CTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTT
TTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA
GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGT
AACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGC
CGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTC
GCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTG
TCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGT
CGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACC
TACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCT
TCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAA
CAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTAT
AGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATG
CTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTT
TACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTC
pENTR221
Name: pENTR221
Antibiotic: kanamycin
Concentration: 10ug/ml
Source: Invitrogen Life Technologies
Vector type: Gateway entry vector
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
CGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTC
GTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATG
GATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCG
GGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAA
CAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGC
AGGCT-linker-ORF-linker-ACCCAGCTTTCTTGTACAAAGTTGGC
ATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTAT
CAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTG
AGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCT
CAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAAC
AATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCC
ATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCA
Full Sequence:
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACC
GCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAG
CGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTC
TCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCC
GACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAG
CCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTT
CTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACC
CTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTC
CTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCA
GTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACT
CTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAAC
GACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGA
TAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATG
CCAACTTTGTACAAAAAAGCAGGCT-linker-ORF-linker-ACCCAG
CTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGT
TGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCC
AGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCT
GGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGAT
AAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAA
TACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGAT
TAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGAT
AATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGA
TGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATG
TTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTT
CCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCAC
CACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTG
ATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTG
CATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCG
TCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTG
ATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAA
ATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGA
TTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTA
TTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATC
CTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTT
TCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATT
TGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACAC
TGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAA
ATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAG
AAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGC
TGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGA
TCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGC
AGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTC
AAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACC
AGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAA
GACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCG
TGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCT
ACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGG
ACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAG
CTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCA
CCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCC
TATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGC
TGGCCTTTTGCTC
pENTR223
Name: pENTR223
Antibiotic: spectinomycin
Concentration: 100 ug/ml
Source: Dana Farber Cancer Institute/Vidal Lab
Vector type: Gateway entry vector
Vector Map: Available Here
Sequencing Primers: M13(-21), t7
Polylinker Sequence:
CAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT
TTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTT
TCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCA
AATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTG
ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTG-link
er-ORF-linker-CCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAG
CATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAA
AATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACA
TGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGA
TGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAG
CCAGGACAGAAATGCCTCGACTTCGCTGC
Full Sequence:
CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG
AGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCA
GTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGC
GCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGA
AAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAA
GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTA
GTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGG
CCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCA
GGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCC
GACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGT
TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC
AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC
CTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTT
TGTACAAAAAAGTTG-linker-ORF-linker-CCAACTTTCTTGTACA
AAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAG
GTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCC
CTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGC
CCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCA
TCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCT
ACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAA
CCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGT
ATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAA
CGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTAC
AGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGA
TGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCC
TAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGA
CTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACG
TTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCC
ACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAA
CAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCT
GGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGA
CGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAG
AATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATC
GACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGC
CTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGG
ATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCC
GACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTG
GTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACT
GGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCT
AGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGA
TCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAG
TCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGT
TACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATC
TTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAA
AACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACT
CTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGT
CCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAC
CGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGT
GGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA
TAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT
TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGA
GAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAG
CGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACG
CCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGT
CGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAG
CAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACA
TGTT
pENTR223.1
Name: pENTR223.1
Antibiotic: spectinomycin
Concentration: 100 ug/ml
Source: Invitrogen Life Technologies
Vector type: Gateway entry vector
Vector Map: Available Here
Sequencing Primers: M13(-21), t7
Polylinker Sequence:
GCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTC
TTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGT
TCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTA
CAAAAAAGCAGAAG-linker-ORF-linker-GCCCAGCTTTCTTGTAC
AAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACA
GGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCC
CCTATAGTGAGTCGTATTACATGGTCAT
Full Sequence:
pENTR223.1-LC
Name: pENTR223.1-LC
Antibiotic: spectinomycin
Concentration: 100 ug/ml
Source: Blue Heron
Vector type: Gateway entry vector
Comments: The pENTR223.1-LC vector has the pBR ORI. Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pENTR223.1-LC.doc ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13F
Polylinker Sequence:
GTACAAAAAAGCAGAAG- ggccgtcaaggcccacc(linker)-ORF-
ggcctcatgg(linker)-GCCCAGCTTTCTTGTAC
Full Sequence:
AAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCA
AGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC
CCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGC
TCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGG
CTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCA
GCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTAC
AGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGT
AGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT
TTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAA
GGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT
AAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTT
GGTCTGACAGTTACCAATGGGTCTCTCGCGTACCGCTAGCCAGGAAGAGT
TTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTT
GATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGT
TGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAG
AGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACT
GAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAAC
GCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTC
TTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGT
TCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTA
CAAAAAAGCTGAACGAGAACGTAAAATGATATAAATATCAATATATTAAA
TTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATA
TCCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTAGTCG
ACCGACAGCCTTCCAAATGTTCTTCGGGTGATGCTGCCAACTTAGTCGAC
CGACAGCCTTCCAAATGTTCTTCTCAAACGGAATCGTCGTATCCAGCCTA
CTCGCTATTGTCCTCAATGCCGTATTAAATCATAAAAAGAAATAAGAAAA
AGAGGTGCGAGCCTCTTTTTTGTGTGACAAAATAAAAACATCTACCTATT
CATATACGCTAGTGTCATAGTCCTGAAAATCATCTGCATCAAGAACAATT
TCACAACTCTTATACTTTTCTCTTACAAGTCGTTCGGCTTCATCTGGATT
TTCAGCCTCTATACTTACTAAACGTGATAAAGTTTCTGTAATTTCTACTG
TATCGACCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCC
CCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGA
GATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCG
GTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAG
TTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCA
CCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAAC
AGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTC
ACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGG
GCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACG
CAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATAT
TGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCA
CTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTAT
ACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCA
TACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCC
CTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACAT
GGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGC
ACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAA
GAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCC
AGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAA
TAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAG
AAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTT
CAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATC
ACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCAT
CAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTT
TCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTA
TAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATG
CCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTT
TAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGT
AGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATC
AACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAG
GGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATT
TATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTAGTCGACTACAG
GTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGGATATGTTGTG
TTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATA
TTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGT
TGGCATTATAAGAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCA
CTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTAT
AGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGT
GTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCAT
GCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCC
AAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCA
GTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGC
GCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGC
GCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTC
TATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTT
TGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAA
ACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCC
pENTR223.1-Sfi
Name: pENTR223.1-Sfi
Antibiotic: spectinomycin
Concentration: 100 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Comments: same as pENTR223.1; Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pENTR223.1-Sfi-map.doc ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13F, T7 Rev
Polylinker Sequence:
GAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAAC
GCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTC
TTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGT
TCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTA
CAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGG
GAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGAT
GTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGA
CCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCG
ATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACG
TGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATAT
CACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTG
TAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGC
CGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCC
GCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGT
GCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATA
GCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCT
TTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAA
AATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGAT
CCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTC
ATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCT
GAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCC
ATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATC
AAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCT
CAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACA
TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACT
CCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAG
GGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGG
AATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGG
ATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATAT
CCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCC
TCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCC
AGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATA
ACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTG
GAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCA
GGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGT
ACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAA
CAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATAT
CCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCT
GGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATA
Full Sequence:
CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCC
GAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAG
TGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACA
TACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAA
GTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGC
AGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGA
ACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGC
CACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGG
TCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT
CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT
GTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG
CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTT
CCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTG
AGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGA
GCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGT
TGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGC
GGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGT
TTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTT
GATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGT
TGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAG
AGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACT
GAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAAC
GCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTC
TTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGT
TCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTA
CAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGG
GAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGAT
GTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGA
CCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCG
ATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACG
TGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATAT
CACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTG
TAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGC
CGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCC
GCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGT
GCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATA
GCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCT
TTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAA
AATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGAT
CCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTC
ATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCT
GAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCC
ATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATC
AAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCT
CAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACA
TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACT
CCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAG
GGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGG
AATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGG
ATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATAT
CCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCC
TCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCC
AGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATA
ACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTG
GAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCA
GGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGT
ACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAA
CAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATAT
CCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCT
GGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATA
TCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCT
GCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCAC
GAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAG
CGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGG
TAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGG
TACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGT
CGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCG
CCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTAT
CGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCG
ACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAA
GCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATG
AAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCC
CCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCA
CGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTG
GAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACG
ATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGT
TGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAAC
AGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCG
CCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCAT
TTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCG
ACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAA
GCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGC
AGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGG
TAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTG
AGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGG
ATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA
AAAAACCACCG
pET-28a
Name: pET-28a
Antibiotic: kanamycin
Concentration: 10ug/ml
Source: Novagen
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: pET Upstream Primer, T7 Terminator Primer
pET-28b
Name: pET-28b
Antibiotic: kanamycin
Concentration: 10ug/ml
Source: Novagen
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: pET Upstream Primer, T7 Terminator Primer
pExpress-1
Name: pExpress-1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Express Genomics/Gruber
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
gcagttttcccagtcacgacgttgtaaaacgacggccagtgcctagctta
taatacgactcactatagggaccactcctcgaggttaattaagcggccgc
cc...cDNA
insert...atccgggaattccggaccgaaacttgttctatagtgtcacc
taaataggcctaatggtcatagctgtttcctgtgtgaaattgttatccgc
t
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcaggatcgatccagacatgataagatacattga
tgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttattt
gtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaat
aaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggg
ggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggta
tggctgattatgatcatgaacagactgtgaggactgaggggcctgaaatg
agccttgggactgtgaatctaaaatacacaaacaattagaatcactagct
cctgtgtataatattttcataaatcatactcagtaagcaaaactctcaag
cagcaagcatatgcagctagtttaacacattatacacttaaaaattttat
atttaccttagagctttaaatctctgtaggtagtttgtccaattatgtca
caccacagaagtaaggttccttcacaaagatcccaagctagcagttttcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggaccactcctcgaggttaattaagcggccgcgatatcccgg
gaattccggaccgaaacttgttctatagtgtcacctaaataggcctaatg
gtcatagctgtttcctgtgtgaaattgttatccgctccgcggcctaggct
agagtccggaggctggatcggtcccggtgtcttctatggaggtcaaaaca
gcgtggatggcgtctccaggcgatctgacggttcactaaacgagctctgc
ttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggg
gcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaa
caaactcccattgacgtcaatggggtggagacttggaaatccccgtgagt
caaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatg
gtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtccca
taaggtcatgtactgggcataatgccaggcgggccatttaccgtcattga
cgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaag
tgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccc
tattggcgttactatgggaacatacgtcattattgacgtcaatgggcggg
ggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacg
acatgcatctaatgagtgaaagggcctcgtactacgcctatttttatagg
ttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcgg
ggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaa
tatgtatccgctcatgagacaataaccctgataaatgcttcaataatatt
gaaaaacgcgcgaattgcaagctctgcattaatgaatcggccaacgcgcg
gggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactga
ctcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaa
aggcggtaatacggttatccacagaatcaggggataacgcaggaaagaac
atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgtt
gctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccag
gcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgcc
gcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgcttt
ctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcc
aagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctt
atccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgc
cactggcagcagccactggtaacaggattagcagagcgaggtatgtaggc
ggtgctacagagttcttgaagtggtggcctaactacggctacactagaag
gacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaa
gagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggt
ttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaga
agatcctttgatcttttctacggggtctgacgctcagtggaacgaaaact
cacgttaagggattttggtcatgagattatcaaaaaggatcttcacctag
atccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatga
gtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatct
cagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtg
tagataactacgatacgggagggcttaccatctggccccagtgctgcaat
gataccgcgagacccacgctcaccggctccagatttatcagcaataaacc
agccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcc
tccatccagtctattaattgttgccgggaagctagagtaagtagttcgcc
agttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgt
cacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatca
aggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt
cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactca
tggttatggcagcactgcataattctcttactgtcatgccatccgtaaga
tgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtg
tatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccg
cgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcg
gggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgta
acccactcgtgcacccaactgatcttcagcatcttttactttcaccagcg
tttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaata
agggcgacacggaaatgttgaatactcatactcttcctttttcaatatta
ttgaagcatttatcagggttattgtctcatgagcggatacatatttgaat
gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaa
gtgccacctgaaattgtaaacgttaatattttgttaaaattcgcgttaaa
tttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaa
tcccttataaatcaaaagaatagaccgagatagggttgagtgttgttcca
gtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagg
gcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccct
aatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccct
aaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggc
gagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaa
gtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcg
ccgctacagggcgcgtc
pFLC1
Name: pFLC1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: RIKEN/Carninci
Vector type: phagemid
Comments: this vector is identical to pBluescriptR used by Mike Brownstein
pGA1
Name: pGA1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA1-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat
tggggcgcgccagcgatgtaagagctccaccgcggtggcggccgctctag
cccgggcggatcccccgggctgcaggaattcgatatcaagcttatcgata
ccgtcgacctcgagggggggcccggtaccttacatcgcgttaattaatcc
agcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatg
gtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacaca
catacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtga
gctaactcaattaattgcgttgcgctcactgcccgctttccagtcgggaa
acctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggc
ggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcg
ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaa
tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagca
aaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtt
tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaa
gtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc
cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgg
atacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagct
cacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggc
tgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa
ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcag
cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctaca
gagttcttgaagtggtggcctaactacggctacactagaaggacagtatt
tggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggta
gctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtt
tgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttt
gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag
ggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta
aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttg
gtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatct
gtctatttcgttcatccatagttgcctgactccccgtcgtgtagataact
acgatacgggagggcttaccatctggccccagtgctgcaatgataccgcg
agacccacgctcaccggctccagatttatcagcaataaaccagccagccg
gaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag
tctattaattgttgccgggaagctagagtaagtagttcgccagttaatag
tttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtt
acatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctcc
gatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatgg
cagcactgcataattctcttactgtcatgccatccgtaagatgcttttct
gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcg
accgagttgctcttgcccggcgtcaatacgggataataccgcgccacata
gcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaa
ctctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcg
tgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggt
gagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgaca
cggaaatgttgaatactcatactcttcctttttcaatattattgaagcat
ttatcagggttattgtctcatgagcggatacatatttgaatgtatttaga
aaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac
pGA14
Name: pGA14
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA14-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgtcaattaaccctcactaaagggaacaa
aagctgttaattaactaaggtacctggccggcctgcatgggccctcgagg
tcgacggtatcgataagcttgatatcgaattcctgcagggatccgcccgg
gctagacgccggcggccgccaccgctggagctcttatggcgcgcccaatt
cgccctatagtgagtcgtattacgtcgcgcttggcgtaatcatggtcata
gctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatac
gagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaa
ctcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacct
gtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtt
tgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcg
gtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacg
gttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaag
gccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttc
cataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtca
gaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg
gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatac
ctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacg
ctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtg
tgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactat
cgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagc
cactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt
tcttgaagtggtggcctaactacggctacactagaagaacagtatttggt
atctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctc
ttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgca
agcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatc
ttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggat
tttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt
aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct
gacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtct
atttcgttcatccatagttgcctgactccccgtcgtgtagataactacga
tacgggagggcttaccatctggccccagtgctgcaatgataccgcgagac
ccacgctcaccggctccagatttatcagcaataaaccagccagccggaag
ggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta
ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttg
cgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtt
tggtatggcttcattcagctccggttcccaacgatcaaggcgagttacat
gatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatc
gttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagc
actgcataattctcttactgtcatgccatccgtaagatgcttttctgtga
ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccg
agttgctcttgcccggcgtcaatacgggataataccgcgccacatagcag
aactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactct
caaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgca
cccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagc
aaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgga
aatgttgaatactcatactcttcctttttcaatattattgaagcatttat
cagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaa
taaacaaataggggttccgcgcacatttccccgaaaagtgccac
pGA15
Name: pGA15
Antibiotic: kanamycin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA15-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagctcactcattaggcaccccaggctttacact
ttatgcttccgcggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacaggaaacagctatgaccatgattacgccactagggcgcgccag
cgatgtaagagctccaccgcggtggcggccgctctagcccgggcggatcc
cccgggctgcaggaattcgatatcaagcttatcgataccgtcgacctcga
gggggggcccggtaccttacatcgcgttaattaactagtggatcgatccc
caattcgccctatagtgagtcgtattacaattcactggccgtcgttttac
aacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgca
gcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccga
tcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgc
ggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaa
agcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtg
gtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgc
tcctttcgctttcttcccttcctttctcgccacgttcgccggctttcccc
gtcaagctctaaatcggggtgggccaacgccctgatagacggtttttcgc
cctttgacgttggagtccacgttctttaatagtggactcttgttccaaac
tggaacaacactcaactctatctcgggctattcttttgatttataaggga
ttttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaa
tttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcac
tctcagtacaatctgctctgatgccgcatagttaagccagccccgacacc
cgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatcc
gcttacagacaagctgtgaccatctccgggagctgcatgtgtcagaggtt
ttcaccgtcatcaccgaaacgcgcgagaggaaagggcctcgtgatacgcc
tatttttataggttaatgtcatgggggggggcacgttgtgtctcaaaatc
tctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaa
ctgtctgcttacataaacagtaatacaaggggtgttatgagccatattca
acgggaaacgtcttgctcgacgaggccgcgattaaattccaacatggatg
ctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggt
gcgacaatctaccgattgtatgggaagcccgatgcgccagagttgtttct
gaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtca
gactaaactggctgacggaatttatgcctcttccgaccatcaagcatttt
atccgtactcctgatgatgcatggttactcaccactgcgatccctgggaa
aacagcattccaggtattagaagaatatcctgattcaggtgaaaatattg
ttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgt
aattgtccttttaacagcgatcgcgtatttcgcctcgctcaggcgcaatc
acgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgta
atggctggcctgttgaacaagtctggaaagaaatgcataagctcttgcca
ttctcaccggattcagtcgtcactcatggtgatttctcacttgataacct
tatttttgacgaggggaaattaataggttgtattgatgttggacgagtcg
gaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggt
gagttttctccttcattacagaaacggctttttcaaaaatatggtattga
taatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttt
tctaatcagaattggttaattggttgtaacactggcagagcattacgctg
acttgacgggacggcgcaagctcatgaccaaaatcccttaacgtgagttt
tcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttg
agatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccac
cgctaccagcggtggtttgtttgccggatcaagagctaccaactcttttt
ccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttct
agtgtagccgtagttaggccaccacttcaagaactctgtagcaccgccta
catacctcgctctgctaatcctgttaccagtggctgctgccagtggcgat
aagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggc
gcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagc
gaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagc
gccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggt
atctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattt
ttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgc
ggcctttttacggttcctggccttttgctggccttttgctcacatgttct
ttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgag
tgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagt
gagcgaggaagcggaag
pGA18
Name: pGA18
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA18-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacaggccaaccatcaccctaatcaa
gttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaaggg
agcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaa
ggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtag
cggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgcta
cagggcgctcccattcgccattcaggctgcgcaactgttgggaagggcgt
ttcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtg
ctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgt
tgtaaaacgacggccagtgagcgcgacgtaatacgactcactatagggcg
aattgggcgcgccataagagctccagcggtggcggccgccggcgtctagc
ccgggcggatccctgcaggaattcgatatcaagcttatcgataccgtcga
cctcgagggcccatgcaggccggccaggtaccttagttaattaacagctt
ttgttccctttagtgagggttaattgacgcgcttggcgtaatcatggtca
tagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacat
acgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagct
aactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaac
ctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcgg
tttgcgtattgggcgctctccgcttcctcgctcactgactcgctgcgctc
ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatac
ggttatccacagaatcaggggataacgcaggaaagaccatgtgagcaaaa
ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttttt
ccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtc
agaggtggcgaaacccgacaggactataaagataccaggcgtttccccct
ggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggata
cctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac
gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt
gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaacta
tcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcag
ccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagag
ttcttgaagtggtggcctaactacggctacactagaagaacagtatttgg
tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagct
cttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc
aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgat
cttttctacggggtctgacgctcagtggaacgaaaactcacgttaaggga
ttttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat
taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtc
tgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtc
tatttcgttcatccatagttgcctgactccccgtcgtgtagataactacg
atacgggagggcttaccatctggccccagtgctgcaatgataccgcgaga
cccacgctcaccggctccagatttatcagcaataaaccagccagccggaa
gggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct
attaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgt
ttggtatggcttcattcagctccggttcccaacgatcaaggcgagttaca
tgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgat
cgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag
cactgcataattctcttactgtcatgccatccgtaagatgcttttctgtg
actggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgacc
gagttgctcttgcccggcgtcaatacgggataataccgcgccacatagca
gaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactc
tcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgc
acccaactgatcttcagcatcttttactttcaccagcgtttctgggtgag
caaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgg
aaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaa
ataaacaaataggggttccgcgcacatttccccgaaaagtgccac
pGA19
Name: pGA19
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA19-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggctatggcccactacaggccaaccatcaccctaatcaa
gttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaaggg
agcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaa
ggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtag
cggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgcta
cagggcgctcccattcgccattcaggctgcgcaactgttgggaagggcgt
ttcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtg
ctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgt
tgtaaaacgacggccagtgagcgcgtcaattaaccctcactaaagggaac
aaaagctgttaattaactaaggtacctggccggcctgcatgggccctcga
ggtcgacggtatcgataagcttgatatcgaattcctgcagggatccgccc
gggctagacgccggcggccgccaccgctggagctcttatggcgcgcccaa
ttcgccctatagtgagtcgtattacgtcgcgcttggcgtaatcatggtca
tagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacat
acgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagct
aactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaac
ctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcgg
tttgcgtattgggcgctctccgcttcctcgctcactgactcgctgcgctc
ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatac
ggttatccacagaatcaggggataacgcaggaaagaccatgtgagcaaaa
ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttttt
ccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtc
agaggtggcgaaacccgacaggactataaagataccaggcgtttccccct
ggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggata
cctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac
gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt
gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaacta
tcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcag
ccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagag
ttcttgaagtggtggcctaactacggctacactagaagaacagtatttgg
tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagct
cttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc
aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgat
cttttctacggggtctgacgctcagtggaacgaaaactcacgttaaggga
ttttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat
taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtc
tgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtc
tatttcgttcatccatagttgcctgactccccgtcgtgtagataactacg
atacgggagggcttaccatctggccccagtgctgcaatgataccgcgaga
accacgctcaccggctccagatttatcagcaataaaccagccagccggaa
gggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct
attaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgt
ttggtatggcttcattcagctccggttcccaacgatcaaggcgagttaca
tgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgat
cgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag
cactgcataattctcttactgtcatgccatccgtaagatgcttttctgtg
actggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgacc
gagttgctcttgcccggcgtcaatacgggataataccgcgccacatagca
gaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactc
tcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgc
acccaactgatcttcagcatcttttactttcaccagcgtttctgggtgag
caaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgg
aaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaa
ataaacaaataggggttccgcgcacatttccccgaaaagtgccac
pGA24
Name: pGA24
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA24-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggctatggcccactacaggccaaccatcaccctaatcaa
gttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaaggg
agcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaa
ggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtag
cggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgcta
cagggcgctcccattcgccattcaggctgcgcaactgttgggaagggcgt
ttcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtg
ctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgt
tgtaaaacgacggccagtgagcgcgtcaattaaccctcactaaagggaac
aaaagctgttaattaactaaggtacctggccggcctgcatgggccctcga
ggtcgacggtatcgataagcttgatatcgaattcctgcagggatccgccc
gggctagacgccggcggccgccaccgctggagctcttatggcgcgcccaa
ttcgccctatagtgagtcgtattacgtcgcgcttggcgtaatcatggtca
tagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacat
acgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagct
aactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaac
ctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcgg
tttgcgtattgggcgctctccgcttcctcgctcactgactcgctgcgctc
ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatac
ggttatccacagaatcaggggataacgcaggaaagaccatgtgagcaaaa
ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttttt
ccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtc
agaggtggcgaaacccgacaggactataaagataccaggcgtttccccct
ggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggata
cctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac
gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt
gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaacta
tcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcag
ccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagag
ttcttgaagtggtggcctaactacggctacactagaagaacagtatttgg
tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagct
cttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc
aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgat
cttttctacggggtctgacgctcagtggaacgaaaactcacgttaaggga
ttttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat
taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtc
tgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtc
tatttcgttcatccatagttgcctgactccccgtcgtgtagataactacg
atacgggagggcttaccatctggccccagtgctgcaatgataccgcgaga
accacgctcaccggctccagatttatcagcaataaaccagccagccggaa
gggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct
attaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgt
ttggtatggcttcattcagctccggttcccaacgatcaaggcgagttaca
tgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgat
cgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag
cactgcataattctcttactgaatagtgtatgcggcgaccgagttgctct
tgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaa
agtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct
taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactga
tcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacagg
aaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaa
tactcatactcttcctttttcaatattattgaagcatttatcagggttat
tgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaat
aggggttccgcgcacatttccccgaaaagtgccac
pGA4
Name: pGA4
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA4-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggctatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat
tgggtaccgggccccccctcgaggtcgacggtatcgataagcttgatatc
gaattcctgcagcccgggggatccgcccgggctagagcggccgccaccgc
ggtggagctccagcttttgttccctttagtgagggttaattgcgcgcttg
gcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcac
aattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgct
ttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg
cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctca
ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac
tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg
cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg
ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg
ccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta
tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaagaacagtatttggtatctgcgctctgctgaagccagttaccttcgga
aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa
aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgt
cgtgtagataactacgatacgggagggcttaccatctggccccagtgctg
caatgataccgcgagaaccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc
cgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt
cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg
atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct
ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatca
ctcatggttatggcagcactgcataattctcttactgtcatgccatccgt
aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat
accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga
tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg
aataagggcgacacggaaatgttgaatactcatactcttcctttttcaat
attattgaagcatttatcagggttattgtctcatgagcggatacatattt
gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg
aaaagtgccac
pGA7
Name: pGA7
Antibiotic: kanamycin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGA7-MCS.pdf ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagctcactcattaggcaccccaggctttacact
ttatgcttccgcggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacaggaaacagctatgaccatgattacgccactagggcgcgccag
cgatgtaagagctccaccgcggtggcggccgctctagcccgggcggatcc
cccgggctgcaggaattcgatatcaagcttatcgataccgtcgacctcga
gggggggcccggtaccttacatcgcgttaattaactagtggatcgatccc
caattcgccctatagtgagtcgtattacaattcactggccgtcgttttac
aacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgca
gcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccga
tcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgc
ggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaa
agcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtg
gtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgc
tcctttcgctttcttcccttcctttctcgccacgttcgccggctttcccc
gtcaagctctaaatcggggtgggccatcgccctgatagacggtttttcgc
cctttgacgttggagtccacgttctttaatagtggactcttgttccaaac
tggaacaacactcaaccctatctcgggctattcttttgatttataaggga
ttttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaa
tttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcac
tctcagtacaatctgctctgatgccgcatagttaagccagccccgacacc
cgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatcc
gcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggtt
ttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcc
tatttttataggttaatgtcatgggggggggcacgttgtgtctcaaaatc
tctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaa
ctgtctgcttacataaacagtaatacaaggggtgttatgagccatattca
acgggaaacgtcttgctcgacgaggccgcgattaaattccaacatggatg
ctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggt
gcgacaatctaccgattgtatgggaagcccgatgcgccagagttgtttct
gaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtca
gactaaactggctgacggaatttatgcctcttccgaccatcaagcatttt
atccgtactcctgatgatgcatggttactcaccactgcgatccctgggaa
aacagcattccaggtattagaagaatatcctgattcaggtgaaaatattg
ttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgt
aattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatc
acgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgta
atggctggcctgttgaacaagtctggaaagaaatgcataagctcttgcca
ttctcaccggattcagtcgtcactcatggtgatttctcacttgataacct
tatttttgacgaggggaaattaataggttgtattgatgttggacgagtcg
gaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggt
gagttttctccttcattacagaaacggctttttcaaaaatatggtattga
taatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttt
tctaatcagaattggttaattggttgtaacactggcagagcattacgctg
acttgacgggacggcgcaagctcatgaccaaaatcccttaacgtgagttt
tcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttg
agatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccac
cgctaccagcggtggtttgtttgccggatcaagagctaccaactcttttt
ccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttct
agtgtagccgtagttaggccaccacttcaagaactctgtagcaccgccta
catacctcgctctgctaatcctgttaccagtggctgctgccagtggcgat
aagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggc
gcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagc
gaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagc
gccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggt
atctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattt
ttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgc
ggcctttttacggttcctggccttttgctggccttttgctcacatgttct
ttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgag
tgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagt
gagcgaggaagcggaag
pGH
Name: pGH
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: phagemid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pGH.doc ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat
tgggtacggccgtcaaggccaagcttcccgggatatcacgtgaagcttgc
ggccgcacatgggccaagctccagcttttgttccctttagtgagggttaa
ttgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgt
tatccgctcacaattccacacaacatacgagccggaagcataaagtgtaa
agcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgct
cactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatga
atcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgc
ttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg
tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggat
aacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccg
taaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacg
agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga
ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcc
tgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg
gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtg
taggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcc
cgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaa
gacacgacttatcgccactggcagcagccactggtaacaggattagcaga
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaacta
cggctacactagaaggacagtatttggtatctgcgctctgctgaagccag
ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccacc
gctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaa
aaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa
aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaat
ctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca
gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcc
tgactccccgtcgtgtagataactacgatacgggagggcttaccatctgg
ccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatt
tatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcct
gcaactttatccgcctccatccagtctattaattgttgccgggaagctag
agtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgcta
caggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctcc
ggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaa
agcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccg
cagtgttatcactcatggttatggcagcactgcataattctcttactgtc
atgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtc
attctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaa
tacgggataataccgcgccacatagcagaactttaaaagtgctcatcatt
ggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgag
atccagttcgatgtaacccactcgtgcacccaactgatcttcagcatctt
ttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgcc
gcaaaaaagggaataagggcgacacggaaatgttgaatactcatactctt
cctttttcaatattattgaagcatttatcagggttattgtctcatgagcg
gatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgc
acatttccccgaaaagtgccacatttcccc
pMA
Name: pMA
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pMA.doc ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
ggccgtcaaggcctaggcgcgccatgagctctaaagcttcgctcgagctg
cggccgcacggatcctcgaattcccaagcttatctcgagtagcggccgct
tggatcccagaattctaggtacctcttaattaactggcctcatgggcc
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtggccgctacagggcgct
cccattcgccattcaggctgcgcaactgttgggaagggcgtttcggtgcg
ggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggc
gattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccagtgagcgcgacgtaatacgactcactatagggcgaattggcgg
aaggccgtcaaggcctaggcgcgccatgagctctaaagcttcgctcgagc
tgcggccgcacggatcctcgaattcccaagcttatctcgagtagcggccg
cttggatcccagaattctaggtacctcttaattaactggcctcatgggcc
ttccgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgca
ttaacatggtcatagctgtttccttgcgtattgggcgctctccgcttcct
cgctcactgactcgctgcgctcggtcgttcgggtaaagcctggggtgcct
aatgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttg
ctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcg
acgctcaagtcagaggtggcgaaacccgacaggactataaagataccagg
cgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg
cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttc
tcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcca
agctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctta
tccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcc
actggcagcagccactggtaacaggattagcagagcgaggtatgtaggcg
gtgctacagagttcttgaagtggtggcctaactacggctacactagaaga
acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaag
agttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt
tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaa
gatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactc
acgttaagggattttggtcatgagattatcaaaaaggatcttcacctaga
tccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgag
taaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctc
agcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgt
agataactacgatacgggagggcttaccatctggccccagtgctgcaatg
ataccgcgagaaccacgctcaccggctccagatttatcagcaataaacca
gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcct
ccatccagtctattaattgttgccgggaagctagagtaagtagttcgcca
gttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtc
acgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaa
ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttc
ggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcat
ggttatggcagcactgcataattctcttactgtcatgccatccgtaagat
gcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgt
atgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgc
gccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcgg
ggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaa
cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgt
ttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataa
gggcgacacggaaatgttgaatactcatactcttcctttttcaatattat
tgaagcatttatcagggttattgtctcatgagcggatacatatttgaatg
tatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaag
tgccac
pME18S-FL3
Name: pME18S-FL3
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: University of Tokyo/Sugano lab
Vector type: phagemid
Has Custom Primer: yes
Vector Map: Available Here
Comments: DraIII sites are lost in cDNA cloning and the XhoI sites should be used to isolate the cDNA insert.
Polylinker Sequence:
tgtacggaagtgttacttctgctctaaaagctgcggaattcctcgagcac
tgtgtgatatccattgtgctggcgcggattctttatcactgataagttgg
tggacatattatgtttatcagtgataaagtgtcaagcatgacaaagttgc
agccgaatacagtgatccgtgccgccctagacctgttgaacgaggtcggc
gtagacggtctgacgacacgcaaactggcggaacggttgggggttcagca
gccggcgctttactggcacttcaggaacaagcgggcgctgctcgacgcac
tggccgaagccatgctggcggagaatcatagcacttcggtgccgagagcc
gacgacgactggcgctcatttctgactgggaatgcccgcagcttcaggca
ggcgctgctcgcctaccgccagcacaatggcaccatgtgctcgagctgca
ggtcgcggccgctaga
Full Sequence:
AAGCTTGGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGC
TCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAAC
CAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCA
TGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATC
CCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACT
AATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTAT
TCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAG
CTCCTCCGATCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTA
CCTGAGGCCGCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCC
TGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAG
GTCGAGACCGGGCCTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCA
GCCGGCTCTCCACGCTTTGCCTGACCCTGCTTGCTCAACTCTACGTCTTT
GTTTCGTTTTCTGTTCTGCGCCGTTACAGATCCAAGCTCTGAAAAACCAG
AAAGTTAACTGGTAAGTTTAGTCTTTTTGTCTTTTATTTCAGGTCCCGGA
TCCGGTGGTGCAAATCAAAGAACTGCTCCTCAGTGGATGTTGCCTTTACT
TCTAGGCCTGTACGGAAGTGTTACTTCTGCTCTAAAAGCTGCGGAATTCC
TCGAGCACTGTGTGATATCCATTGTGCTGGCGCGGATTCTTTATCACTGA
TAAGTTGGTGGACATATTATGTTTATCAGTGATAAAGTGTCAAGCATGAC
AAAGTTGCAGCCGAATACAGTGATCCGTGCCGCCCTAGACCTGTTGAACG
AGGTCGGCGTAGACGGTCTGACGACACGCAAACTGGCGGAACGGTTGGGG
GTTCAGCAGCCGGCGCTTTACTGGCACTTCAGGAACAAGCGGGCGCTGCT
CGACGCACTGGCCGAAGCCATGCTGGCGGAGAATCATAGCACTTCGGTGC
CGAGAGCCGACGACGACTGGCGCTCATTTCTGACTGGGAATGCCCGCAGC
TTCAGGCAGGCGCTGCTCGCCTACCGCCAGCACAATGGCACCATGTGCTC
GAGCTGCAGGTCGCGGCCGCTAGACTAGTCTAGAGAAAAAACCTCCCACA
CCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACT
TGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAAT
TTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAA
ACTCATCAATGTATCTTATCATGTCTGGATCCCCGGGTACCGAGCTCGAA
TTAATTCCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTT
CGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATC
CACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGG
CTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTG
GCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCC
GCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAG
GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTT
GAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGC
GCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATC
CGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCT
ACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGT
CATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAAT
GAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGT
TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCG
TTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGG
AGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGC
TCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGA
GCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATT
GTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAAC
GTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTAT
GGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCC
CCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTC
AGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCA
TAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTG
AGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGC
TCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTT
AAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGA
TCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAAC
TGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAAC
AGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTT
GAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGT
TATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACA
AATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGC
pMK
Name: pMK
Antibiotic: kanamycin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pMK.doc ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
ggccgtcaaggcctaggcgcgccatgagctctaaagcttcgctcgagctg
cggccgcacggatcctcgaattcccaagcttatctcgagtagcggccgct
tggatcccagaattctaggtacctcttaattaactggcctcatgggcc
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtggccgctacagggcgct
cccattcgccattcaggctgcgcaactgttgggaagggcgtttcggtgcg
ggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggc
gattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccagtgagcgcgacgtaatacgactcactatagggcgaattgaagg
aaggccgtcaaggcctaggcgcgccatgagctctaaagcttcgctcgagc
tgcggccgcacggatcctcgaattcccaagcttatctcgagtagcggccg
cttggatcccagaattctaggtacctcttaattaactggcctcatgggcc
ttcctttcactgcccgctttccagtcgggaaacctgtcgtgccagctgca
ttaacatggtcatagctgtttccttgcgtattgggcgctctccgcttcct
cgctcactgactcgctgcgctcggtcgttcgggtaaagcctggggtgcct
aatgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttg
ctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcg
acgctcaagtcagaggtggcgaaacccgacaggactataaagataccagg
cgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg
cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttc
tcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcca
agctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctta
tccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcc
actggcagcagccactggtaacaggattagcagagcgaggtatgtaggcg
gtgctacagagttcttgaagtggtggcctaactacggctacactagaaga
acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaag
agttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt
tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaa
gatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactc
acgttaagggattttggtcatgagattatcaaaaaggatcttcacctaga
tccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgag
taaacttggtctgacagttattagaaaaattcatccagcagacgataaaa
cgcaatacgctggctatccggtgccgcaatgccatacagcaccagaaaac
gatccgcccattcgccgcccagttcttccgcaatatcacgggtggccagc
gcaatatcctgataacgatccgccacgcccagacggccgcaatcaataaa
gccgctaaaacggccattttccaccataatgttcggcaggcacgcatcac
catgggtcaccaccagatcttcgccatccggcatgctcgctttcagacgc
gcaaacagctctgccggtgccaggccctgatgttcttcatccagatcatc
ctgatccaccaggcccgcttccatacgggtacgcgcacgttcaatacgat
gtttcgcctgatgatcaaacggacaggtcgccgggtccagggtatgcaga
cgacgcatggcatccgccataatgctcactttttctgccggcgccagatg
gctagacagcagatcctgacccggcacttcgcccagcagcagccaatcac
ggcccgcttcggtcaccacatccagcaccgccgcacacggaacaccggtg
gtggccagccagctcagacgcgccgcttcatcctgcagctcgttcagcgc
accgctcagatcggttttcacaaacagcaccggacgaccctgcgcgctca
gacgaaacaccgccgcatcagagcagccaatggtctgctgcgcccaatca
tagccaaacagacgttccacccacgctgccgggctacccgcatgcaggcc
atcctgttcaatcatactcttcctttttcaatattattgaagcatttatc
agggttattgtctcatgagcggatacatatttgaatgtatttagaaaaat
aaacaaataggggttccgcgcacatttccccgaaaagtgccac
pMK-U
Name: pMK-U
Antibiotic: kanamycin
Concentration: 50-200 ug/ml
Source: Geneart
Vector type: plasmid
Comments: Vector Map was previously listed as being here: http://image.hudsonalpha.org/html/libs/pMK-U.doc ; but this file was not present at time of server transfer to CPD.
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
ggccgtcaaggcccctcagcagacatnnnactttcgctgaggggcctcat
gggcc
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtggccgctacagggcgct
cccattcgccattcaggctgcgcaactgttgggaagggcgtttcggtgcg
ggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggc
gattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccagtgagcgcgacgtaatacgactcactatagggcgaattgaagg
aaggccgtcaaggcccctcagcagacatnnnactttcgctgaggggcctc
atgggccttcctttcactgcccgctttccagtcgggaaacctgtcgtgcc
agctgcattaacatggtcatagctgtttccttgcgtattgggcgctctcc
gcttcctcgctcactgactcgctgcgctcggtcgttcgggtaaagcctgg
ggtgcctaatgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggc
cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcaca
aaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaaga
taccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac
cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtgg
cgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgtt
cgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg
cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgact
tatcgccactggcagcagccactggtaacaggattagcagagcgaggtat
gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac
tagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcg
gaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagc
ggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatc
tcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacg
aaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttc
acctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat
atatgagtaaacttggtctgacagttattagaaaaattcatccagcagac
gataaaacgcaatacgctggctatccggtgccgcaatgccatacagcacc
agaaaacgatccgcccattcgccgcccagttcttccgcaatatcacgggt
ggccagcgcaatatcctgataacgatccgccacgcccagacggccgcaat
caataaagccgctaaaacggccattttccaccataatgttcggcaggcac
gcatcaccatgggtcaccaccagatcttcgccatccggcatgctcgcttt
cagacgcgcaaacagctctgccggtgccaggccctgatgttcttcatcca
gatcatcctgatccaccaggcccgcttccatacgggtacgcgcacgttca
atacgatgtttcgcctgatgatcaaacggacaggtcgccgggtccagggt
atgcagacgacgcatggcatccgccataatgctcactttttctgccggcg
ccagatggctagacagcagatcctgacccggcacttcgcccagcagcagc
caatcacggcccgcttcggtcaccacatccagcaccgccgcacacggaac
accggtggtggccagccagctcagacgcgccgcttcatcctgcagctcgt
tcagcgcaccgctcagatcggttttcacaaacagcaccggacgaccctgc
gcgctcagacgaaacaccgccgcatcagagcagccaatggtctgctgcgc
ccaatcatagccaaacagacgttccacccacgctgccgggctacccgcat
gcaggccatcctgttcaatcatactcttcctttttcaatattattgaagc
atttatcagggttattgtctcatgagcggatacatatttgaatgtattta
gaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac
pOTB7
Name: pOTB7
Antibiotic: chloramphenicol
Concentration: 25 ug/ml
Source: UCBerkeley/Rubin
Vector type: plasmid
Vector Map: Available Here
Comments: this vector contains the Gateway recombination cassette in the polylinker.
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcgaattcccttactagtttccctcgaggcatctatgt
cgggtgcggagaaagaggtaatgaaatggcacatggtcatagctgtttcc
tgacccagctttcttgtacaaagtggtagatctgccggtctccctatagt
gagtcgtattaatttcgataagccaggttaacctgcattaatgaatcggc
tgcagtacccgggaatttaacccgcctaatgagcgggctttt
Full Sequence:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcgaattcccttactagtttccctcgaggcatctatgt
cgggtgcggagaaagaggtaatgaaatggcacatggtcatagctgtttcc
tgacccagctttcttgtacaaagtggtagatctgccggtctccctatagt
gagtcgtattaatttcgataagccaggttaacctgcattaatgaatcggc
tgcagtacccgggaatttaacccgcctaatgagcgggcttttttttgtga
tccaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgctt
gcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaag
agctaccaactctttttccgaaggtaactggcttcagcagagcgcagata
ccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaa
ctctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtgg
ctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacga
tagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcac
acagcccagcttggagcgaacgacctacaccgaactgagatacctacagc
gtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacagg
tatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttcc
agggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctct
gacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatgg
aaaaacgccagcaacgcggatcacaacaaaaagcccgctcattaggcggg
ctaaattctcatgtttgacagcttatcatcgataagctagcggccgctag
ctttaatgagttatcgagattttcaggagctaaggaagctaaaatggaga
aaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaa
gaacattttgaggcatttcagtcagttgctcaatgtacctataaccagac
cgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagc
acaagttttatccggcctttattcacattcttgcccgcctgatgaatgct
catccggagttccgtatggcaatgaaagacggtgagctggtgatatggga
tagtgttcacccttgttacaccgttttccatgagcaaactgaaacgtttt
catcgctctggagtgaataccacgacgatttccggcagtttctacacata
tattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaa
agggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtt
tcaccagttttgatttaaacgtggccaatatggacaacttcttcgccccc
gttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgcc
gctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggca
gaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcg
taattggtacgtcga
pOTB7-3
Name: pOTB7-3
Antibiotic: chloramphenicol
Concentration: 10ug/ml
Source: University of Michigan, Ann Arbor
Vector type: phagemid
Comments: Custom vector from pOTB7 parent, the POTB7 vector was modified to produce pOTB7-3 by inserting an F1 origin in the SmaI site at 358 of pOTB7, an AscI restriction site in the BglII site at 278, and the bacterial CCD killer gene flanked by BstXI restriction sites in the XhoI site at 185 (note the M13 reverse primer is lost during cloning).
Sequencing Primers: M13(-21), t7, sp6
Polylinker Sequence:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcgaattcccttactagtttccctcgagcggccgccag
tgtgctggaaagcatgttctttcctgcgttatcccctgattctgtggata
accgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacg
accgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacg
caaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtg
agttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaaca
gctatgaccatgattacgccaagctatttaggtgacactatagaatactc
aagctatgcatcaagcttggtaccgagctcggatccactagtaacggccg
ccagtgtgctggaattctgcagatatccatcacactggcggccgctcgag
gcatctatgtcgggtgcggagaaagaggtaatgaaatggcacatggtcat
agctgtttcctgacccagctttcttgtacaaagt
Full Sequence:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcgaattcccttactagtttccctcgagcggccgccag
tgtgctggaaagcatgttctttcctgcgttatcccctgattctgtggata
accgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacg
accgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacg
caaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtg
agttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaaca
gctatgaccatgattacgccaagctatttaggtgacactatagaatactc
aagctatgcatcaagcttggtaccgagctcggatccactagtaacggccg
ccagtgtgctggaattctgcagatatccatcacactggcggccgctcgag
gcatctatgtcgggtgcggagaaagaggtaatgaaatggcacatggtcat
agctgtttcctgacccagctttcttgtacaaagtggtagatctaaggcgc
gcctgatctgccggtctccctatagtgagtcgtattaatttcgataagcc
aggttaacctgcattaatgaatcggctgcagtacccgggaaattgtaagc
gttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcatt
ttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaat
agaccgagatagggttgagtgttgttccagtttggaacaagagtccacta
ttaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcaggg
cgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcga
ggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttaga
gcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagc
gaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcg
taaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccat
tcgccattcccgggaatttaacccgcctaatgagcgggcttttttttgtg
atccaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgct
tgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaa
gagctaccaactctttttccgaaggtaactggcttcagcagagcgcagat
accaaatactgttcttctagtgtagccgtagttaggccaccacttcaaga
actctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtg
gctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacg
atagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgca
cacagcccagcttggagcgaacgacctacaccgaactgagatacctacag
cgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacag
gtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttc
cagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctc
tgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatg
gaaaaacgccagcaacgcggatcacaacaaaaagcccgctcattaggcgg
gctaaattctcatgtttgacagcttatcatcgataagctagcggccgcta
gctttaatgagttatcgagattttcaggagctaaggaagctaaaatggag
aaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaa
agaacattttgaggcatttcagtcagttgctcaatgtacctataaccaga
ccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataag
cacaagttttatccggcctttattcacattcttgcccgcctgatgaatgc
tcatccggagttccgtatggcaatgaaagacggtgagctggtgatatggg
atagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttt
tcatcgctctggagtgaataccacgacgatttccggcagtttctacacat
atattcgcaagatgtggcgtgttacggtgaaaacctggcctatttcccta
aagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagt
ttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccc
cgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgc
cgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggc
agaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggc
gtaattggtacgtcga
pOTB7a
Name: pOTB7a
Antibiotic: chloramphenicol
Concentration: 25 ug/ml
Source: Edge Biosystems
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13(-21), t7, sp6
Polylinker Sequence:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcctcgagccttccgaattctttcattacctctttctc
cgcaccccacataaagtgcggagaaagaggtaatgaaatggcacccagct
ttcttgtacaagtggtagatctgccggtctccctatagtgagtcgtatta
atttcgataagccaggttaacctgcattaatgaatcggctgcagtacccg
ggaatttaacccgcctaatgagc
Full Sequence:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcctcgagccttccgaattctttcattacctctttctc
cgcaccccacataaagtgcggagaaagaggtaatgaaatggcacccagct
ttcttgtacaagtggtagatctgccggtctccctatagtgagtcgtatta
atttcgataagccaggttaacctgcattaatgaatcggctgcagtacccg
ggaatttaacccgcctaatgagcgggcttttttttgtgatccaaaggatc
ttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaa
aaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaact
ctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgt
tcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcac
cgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagt
ggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccgga
taaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagct
tggagcgaacgacctacaccgaactgagatacctacagcgtgagctatga
gaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaag
cggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacg
cctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgt
cgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccag
caacgcggatcacaacaaaaagcccgctcattaggcgggctaaattctca
tgtttgacagcttatcatcgataagctagcggccgctagctttaatgagt
tatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcact
ggatataccaccgttgatatatcccaatggcatcgtaaagaacattttga
ggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctgg
atattacggcctttttaaagaccgtaaagaaaaataagcacaagttttat
ccggcctttattcacattcttgcccgcctgatgaatgctcatccggagtt
ccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacc
cttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctgg
agtgaataccacgacgatttccggcagtttctacacatatattcgcaaga
tgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattg
agaatatgtttttcgtctcagccaatccctgggtgagtttcaccagtttt
gatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccat
gggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattc
aggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaat
gaattacaacagtactgcgatgagtggcagggcggggcgtaattggtacg
tcga
pPCR-Script Amp SK(+)
Name: pPCR-Script Amp SK(+)
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Stratagene
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
ttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcg
aattgggtaccgggccccccctcgaggtcgacggtatcgataagcttgat
atcgaattcctgcagcccgggggatccgcccgggctagagcggccgccac
cgcggtggagctccagcttttgttccctttagtgagggttaattgcgcgc
ttggcgtaatcatggtcatagctgtttcc
Full Sequence:
ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgtt
aaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttat
aaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaa
caagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaa
ccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagt
tttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggag
cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaagg
aagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg
gtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctaca
gggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgat
cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct
gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg
taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat
tgggtaccgggccccccctcgaggtcgacggtatcgataagcttgatatc
gaattcctgcagcccgggggatccgcccgggctagagcggccgccaccgc
ggtggagctccagcttttgttccctttagtgagggttaattgcgcgcttg
gcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcac
aattccacacaacatacgagccggaagcataaagtgtaaagcctggggtg
cctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgct
ttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacg
cgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctca
ctgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac
tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa
gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg
cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccc
tgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg
ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg
ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg
ccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta
tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacacta
gaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga
aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcgg
tggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc
aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa
aactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac
ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct
atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgt
cgtgtagataactacgatacgggagggcttaccatctggccccagtgctg
caatgataccgcgagacccacgctcaccggctccagatttatcagcaata
aaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatc
cgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt
cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg
atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct
ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatca
ctcatggttatggcagcactgcataattctcttactgtcatgccatccgt
aagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat
accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga
tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaaggg
aataagggcgacacggaaatgttgaatactcatactcttcctttttcaat
attattgaagcatttatcagggttattgtctcatgagcggatacatattt
gaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg
aaaagtgccac
pRKW2
Name: pRKW2
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: DKFZ/Niehrs
Vector type: phagemid
Has Custom Primer: yes
Vector Map: Available Here
Comments: modified from pRK5
Sequencing Primers: M13(-21), M13 reverse, AG195, AG189
Polylinker Sequence:
catacgatttaggtgacactatagaataacatccactttgcctttctctc
cacaggtgtccactcccaggtccaactgcacctcggttctatccgggccc
gaatacaagctacttgttctttttgcactcgag...5'-insert-3'..
.ggatccactagttctagagcggccgccaccgcggtggagcttggccgtg
gcccaacttgtttattgcagcttataatggttacaaataaagcaatagca
tcacaaatttcacaaataaag
Full Sequence:
ttcgagctcgcccgacattgattattgactagttattaatagtaatcaat
tacggggtcattagttcatagcccatatatggagttccgcgttacataac
ttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg
acgtcaataatgacgtatgttcccatagtaacgccaatagggactttcca
ttgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtac
atcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggt
aaatggcccgcctggcattatgcccagtacatgaccttatgggactttcc
tacttggcagtacatctacgtattagtcatcgctattaccatggtgatgc
ggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga
tttccaagtctccaccccattgacgtcaatgggagtttgttttggcacca
aaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgc
aaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgt
ttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacct
ccatagaagacaccgggaccgatccagcctccgcggccgggaacggtgca
ttggaacgcggattccccgtgccaagagtgacgtaagtaccgcctataga
gtctataggcccacccccttggcttcgttagaacgcggctacaattaata
cataaccttatgtatcatacacatacgatttaggtgacactatagaataa
catccactttgcctttctctccacaggtgtccactcccaggtccaactgc
acctcggttctatccgggcccgaatacaagctacttgttctttttgcact
cgag..insert..ggatccactagttctagagcggccgccaccgcggtg
gagcttggccgtggcccaacttgtttattgcagcttataatggttacaaa
taaagcaatagcatcacaaatttcacaaataaagcatttttttcactgca
ttctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgga
tcgatcgggaattaattcggcgcagcaccatggcctgaaataacctctga
aagaggaacttggttaggtaccttctgaggcggaaagaaccagctgtgga
atgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcaga
agtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtc
cccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagt
cagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccg
cccagttccgcccattctccgccccatggctgactaattttttttattta
tgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgag
gaggcttttttggaggcctaggcttttgcaaaaagctgttaacagcttgg
cactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacc
caacttaatcgccttgcagcacatccccccttcgccagctggcgtaatag
cgaagaggcccgcaccgatcgcccttcccaacagttgcgtagcctgaatg
gcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatt
tcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgca
ttaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgc
cagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgcca
cgttcgccggctttccccgtcaagctctaaatcgggggctccctttaggg
ttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttggg
tgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctt
tgacgttggagtccacgttctttaatagtggactcttgttccaaactgga
acaacactcaaccctatctcgggctattcttttgatttataagggatttt
gccgatttcggcctattggttaaaaaatgagctgatttaacaaaaattta
acgcgaattttaacaaaatattaacgtttacaattttatggtgcactctc
agtacaatctgctctgatgccgcatagttaagccaactccgctatcgcta
cgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgc
gccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtga
ccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaa
cgcgcgaggcagtattcttgaagacgaaagggcctcgtgatacgcctatt
tttataggttaatgtcatgataataatggtttcttagacgtcaggtggca
cttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaata
cattcaaatatgtatccgctcatgagacaataaccctgataaatgcttca
ataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccc
ttattcccttttttgcggcattttgccttcctgtttttgctcacccagaa
acgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtggg
ttacatcgaactggatctcaacagcggtaagatccttgagagttttcgcc
ccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggc
gcggtattatcccgtgatgacgccgggcaagagcaactcggtcgccgcat
acactattctcagaatgacttggttgagtactcaccagtcacagaaaagc
atcttacggatggcatgacagtaagagaattatgcagtgctgccataacc
atgagtgataacactgcggccaacttacttctgacaacgatcggaggacc
gaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgcc
ttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgt
gacaccacgatgccagcagcaatggcaacaacgttgcgcaaactattaac
tggcgaactacttactctagcttcccggcaacaattaatagactggatgg
aggcggataaagttgcaggaccacttctgcgctcggcccttccggctggc
tggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtat
cattgcagcactggggccagatggtaagccctcccgtatcgtagttatct
acacgacggggagtcaggcaactatggatgaacgaaatagacagatcgct
gagataggtgcctcactgattaagcattggtaactgtcagaccaagttta
ctcatatatactttagattgatttaaaacttcatttttaatttaaaagga
tctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgt
gagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatc
ttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaa
aaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaact
ctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgt
ccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcac
cgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagt
ggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccgga
taaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagct
tggagcgaacgacctacaccgaactgagatacctacagcgtgagcattga
gaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaag
cggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacg
cctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgt
cgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccag
caacgcggcctttttacggttcctggccttttgctggccttttgctcaca
tgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcc
tttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcga
gtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatccagctggcacgacaggtttcccgac
tggaaagcgggcagtgagcgcaacgcaattaatgtgagttacctcactca
ttaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtg
gaattgtgagcggataacaatttcacacaggaaacagctatgaccatgat
tacgaattaa
pSMART_cDNA
Name: pSMART_cDNA
Antibiotic: kanamycin
Concentration: 50-200 ug/ml
Source: Lucigen Corporation
Vector type: plasmid
Comments: pSMART_cDNA -- A derivative of Lucigen's pSMART vector, containing a pUC19 multiple cloning site. Vector map previously located here: http://www.lucigen.com/catalog/index.php?cPath=14_15_18
Sequencing Primers: pSMART-1777F, pSMART-200R
Polylinker Sequence:
ttcggcgcgcctaaacacataatcagccgagtctttgcaatagtagaaat
tcatcactttctagatcctgaaccctatggacaccttcatcttcctccac
tcgtaatctccttgatactagattctctctatagcaggattcttcgcaca
accagattctcagaaaaaccggtcagagaaaagctaaaccaaacaagtca
aagcacaggaacgaacacatccaagaaacaagtgagaagcaacagttgtt
cagcaaaggtttggtgcggctacaatatttcgagctgattacagataaga
ccaccgagcttatgtgcaacagggtgccctattgtctaaataactaatac
tccccgagtctgaaccgctcgatcgaacctcactcaacactgacgagagc
gagtcgtctaactcttgcagcctctttgcgtcgtgaaagctctctggatc
ctctagagtcgacctgcaggcatgcaagctt
Full Sequence:
CCCGTGAAGGTGAGCCAGTGAGTTGATTGCAGTCCAGTTACGCTAAATTA
ATACGACTCACTATAGGGGAAGATGAATTCGGCGCGCCTAAACACATAAT
CAGCCGAGTCTTTGCAATAGTAGAAATTCATCACTTTCTAGATCCTGAAC
CCTATGGACACCTTCATCTTCCTCCACTCGTAATCTCCTTGATACTAGAT
TCTCTCTATAGCAGGATTCTTCGCACAACCAGATTCTCAGAAAAACCGGT
CAGAGAAAAGCTAAACCAAACAAGTCAAAGCACAGGAACGAACACATCCA
AGAAACAAGTGAGAAGCAACAGTTGTTCAGCAAAGGTTTGGTGCGGCTAC
AATATTTCGAGCTGATTACAGATAAGACCACCGAGCTTATGTGCAACAGG
GTGCCCTATTGTCTAAATAACTAATACTCCCCGAGTCTGAACCGCTCGAT
CGAACCTCACTCAACACTGACGAGAGCGAGTCGTCTAACTCTTGCAGCCT
CTTTGCGTCGTGAAAGCTCTCTGGATCCTCTAGAGTCGACCTGCAGGCAT
GCAAGCTTATCACTGACCATTTAAATCATACCTGACCTCCATAGCAGAAA
GTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGATCGGCACGTAAGAGGT
TCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGA
GTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGAGCCATATTCAAC
GGGAAACGTCTTGTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGAT
TTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGAC
AATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAAC
ATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGGCTA
AACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCG
TACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCAGGGAAAACAG
CATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGAT
GCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTG
TCCTTTTAACGGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAA
TGAATAACGGTTTGGTTGGTGCGAGTGATTTTGATGACGAGCGTAATGGC
TGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGTTATTGCCATTCTC
ACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTT
TTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATC
GCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTT
TTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATC
CTGATATGAATAAATTGCAGTTTCACTTGATGCTCGATGAGTTTTTCTGA
GGGCCCAAATGTAATCACCTGGCTCACCTTCGGGTGGGCCTTTCTGCGTT
GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GATGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAG
GCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCC
GCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC
AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT
ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGC
GGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAG
AACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTCGGAAAAAG
AGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAA
GATCCTTTGATTTTCTACCGAAGAAAGGCCCA
pSPORT1
Name: pSPORT1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
gaagagctatgacgtcgcatgcacgcgtacgtaagcttggatcctctaga
gcggccgc{3' - cDNA insert -
5'}gtcgacccgggaattccggaccggtacctgcaggcgtaccagctt
Full Sequence:
ccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcggg
cctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcga
ttaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgac
ggccagtgaattgaatttaggtgacactatagaagagctatgacgtcgca
tgcacgcgtacgtaagcttggatcctctagagcggccgccgactagtgag
ctcgtcgacccgggaattccggaccggtacctgcaggcgtaccagctttc
cctatagtgagtcgtattagagcttggcgtaatcatggtcatagctgttt
cctgtgtgaaattgttatccgctcacaattccacacaacatacgagccgg
aagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacat
taattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgc
cagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtat
tgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctga
ttgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgct
ggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcggga
tataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatcc
gcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgc
catctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattca
gcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcc
cgttccgctatcggctgaatttgattgcgagtgagatatttatgccagcc
agccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcg
cgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgta
ccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagac
atcaagaaataacgccggaacattagtgcaggcagcttccacagcaatgg
catcctggtcatccagcggatagttaatgatcagcccactgacccgttgc
gcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttc
taccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaa
tcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggca
acgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggtt
gggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttt
tcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataa
gagacaccggcatactctgcgacatcgtataacgttactggtttcacatt
caccaccctgaattgactctcttccgggcgctatcatgccataccgcgaa
aggttttgcgccattcgatggtgtcaacgtaaatgccgcttcgccttcgc
gcgcgaattgcaagctctgcattaatgaatcggccaacgcgcggggagag
gcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctg
cgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggt
aatacggttatccacagaatcaggggataacgcaggaaagaacatgtgag
caaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcg
tttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctc
aagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttc
cccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttacc
ggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatg
ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgg
gctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggt
aactatcgtcttgagtccaacccggtaagacacgacttatcgccactggc
agcagccactggtaacaggattagcagagcgaggtatgtaggcggtgcta
cagagttcttgaagtggtggcctaactacggctacactagaaggacagta
tttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgg
tagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttg
tttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct
ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtta
agggattttggtcatgagattatcaaaaaggatcttcacctagatccttt
taaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaact
tggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgat
ctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataa
ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccg
cgagacccacgctcaccggctccagatttatcagcaataaaccagccagc
cggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcc
agtctattaattgttgccgggaagctagagtaagtagttcgccagttaat
agtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctc
gtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgag
ttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcct
ccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttat
ggcagcactgcataattctcttactgtcatgccatccgtaagatgctttt
ctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcgg
cgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaa
aactctcaaggatcttaccgctgttgagatccagttcgatgtaacccact
cgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgg
gtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcga
cacggaaatgttgaatactcatactcttcctttttcaatattattgaagc
atttatcagggttattgtctcatgagcggatacatatttgaatgtattta
gaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac
ctgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgt
taaatcagctcattttttaaccaataggccgaaatcggcaaaatccctta
taaatcaaaagaatagaccgagatagggttgagtgttgttccagtttgga
acaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaa
accgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaag
ttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaaggga
gcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaag
gaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagc
ggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctac
agggcgcgtc
pSPORT2
Name: pSPORT2
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: phagemid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
cattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggc
ctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat
taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacg
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagtcgacgagctcactagtga
cagcggccgccccgggaattccggaccggtacctgcaggcgtaccagctt
tccctatagtgagtcgtattagagcttggcgtaatcatggtcatagctgt
ttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagcc
ggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcac
attaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgt
Full Sequence:
cattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggc
ctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat
taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacg
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagtcgacgagctcactagtga
cagcggccgccccgggaattccggaccggtacctgcaggcgtaccagctt
tccctatagtgagtcgtattagagcttggcgtaatcatggtcatagctgt
ttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagcc
ggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcac
attaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgt
gccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgt
attgggcgccagggtggtttttcttttcaccagtgagacgggcaacagct
gattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacg
ctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgg
gatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatat
ccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagc
gccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcatt
cagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcctt
cccgttccgctatcggctgaatttgattgcgagtgagatatttatgccag
ccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacag
cgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcg
taccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagag
acatcaagaaataacgccggaacattagtgcaggcagcttccacagcaat
ggcatcctggtcatccagcggatagttaatgatcagcccactgacccgtt
gcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgt
tctaccatcgacaccaccacgctggcacccagttgatcggcgcgagattt
aatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtgg
caacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcgg
ttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgt
tttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgat
aagagacaccggcatactctgcgacatcgtataacgttactggtttcaca
ttcaccaccctgaattgactctcttccgggcgctatcatgccataccgcg
aaaggttttgcgccattcgatggtgtcaacgtaaatgccgcttcgccttc
gcgcgcgaattgcaagctctgcattaatgaatcggccaacgcgcggggag
aggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgc
tgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg
gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtg
agcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgg
cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgc
tcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtt
tccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctta
ccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaa
tgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagct
gggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg
gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactg
gcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgc
tacagagttcttgaagtggtggcctaactacggctacactagaaggacag
tatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt
ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttt
tgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc
ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgt
taagggattttggtcatgccataacttcgtatagcatacattatacgaag
ttatggcatgagattatcaaaaaggatcttcacctagatccttttaaatt
aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct
gacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtct
atttcgttcatccatagttgcctgactccccgtcgtgtagataactacga
tacgggagggcttaccatctggccccagtgctgcaatgataccgcgagac
ccacgctcaccggctccagatttatcagcaataaaccagccagccggaag
ggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta
ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttg
cgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtt
tggtatggcttcattcagctccggttcccaacgatcaaggcgagttacat
gatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatc
gttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagc
actgcataattctcttactgtcatgccatccgtaagatgcttttctgtga
ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccg
agttgctcttgcccggcgtcaatacgggataataccgcgccacatagcag
aactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactct
caaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgca
cccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagc
aaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgga
aatgttgaatactcatactcttcctttttcaatattattgaagcatttat
cagggttattgtctcatgccaggggtgggcacacatatttgataccagcg
atccctacacagcacataattcaatgcgacttccctctatcgcacatctt
agacctttattctccctccagcacacatcgaagctgccgagcaagccgtt
ctcaccagtccaagacctggcatgagcggatacatatttgaatgtattta
gaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac
ctgaaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgt
taaatcagctcattttttaaccaataggccgaaatcggcaaaatccctta
taaatcaaaagaatagaccgagatagggttgagtgttgttccagtttgga
acaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaa
accgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaag
ttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaaggga
gcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaag
gaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagc
ggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctac
agggcgcgtcc
pT7T3D-PacI
Name: pT7T3D-PacI
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Columbia University/Soares
Vector type: plasmid
Vector Map: Available Here
Comments: Modified by Soares from original Pharmacia pT7T3 vector. NOTE: Washington University's trace files indicate that the "c" at 40 bp (directly before the EcoRI site) should be deleted. NOTE: Please do not contact Pharmacia with questions about this vector; it is a modified version of their original pT7T3 vector (which itself is not offered any more by Pharmacia) which they have never carried as a catalog item. If you have questions, please contact help@image.llnl.gov. Also please note that especially in the past, this vector has been known by several aliases, including pT7T3D-Pac and pT7T3D.
Sequencing Primers: M13(-21), M13 reverse, t7, t3
Polylinker Sequence:
tttaatacgactcactatagggaatttggccctcgaggccaagaattccc
gactacgtagtcggggatccgtcttaattaagcggccgcaagcttattcc
ctttagtgagggttaattttagcttggcactggccgtcgttttacaacgt
cgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcaca
tccccctttcgccagctggcgtaatagcgaagag
Full Sequence:
cccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgg
gcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcg
attaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacga
cggccagtgccaagctaaaattaaccctcactaaagggaataagcttgcg
gccgcttaattaagacggatccccgactacgtagtcgggaattcttggcc
tcgagggccaaattccctatagtgagtcgtattaaattcgtaatcatggt
catagctgtttcctgtgtgaaattgttatccgctcacaattccacacaac
atacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgag
ctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaa
acctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggc
ggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcg
ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaa
tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagca
aaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtt
tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaa
gtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc
cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgg
atacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgct
cacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggc
tgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa
ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcag
cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctaca
gagttcttgaagtggtggcctaactacggctacactagaagaacagtatt
tggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggta
gctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtt
tgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttt
gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag
ggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta
aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttg
gtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatct
gtctatttcgttcatccatagttgcctgactccccgtcgtgtagataact
acgatacgggagggcttaccatctggccccagtgctgcaatgataccgcg
agacccacgctcaccggctccagatttatcagcaataaaccagccagccg
gaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag
tctattaattgttgccgggaagctagagtaagtagttcgccagttaatag
tttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtt
acatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctcc
gatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatgg
cagcactgcataattctcttactgtcatgccatccgtaagatgcttttct
gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcg
accgagttgctcttgcccggcgtcaatacgggataataccgcgccacata
gcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaa
ctctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcg
tgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggt
gagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgaca
cggaaatgttgaatactcatactcttcctttttcaatattattgaagcat
ttatcagggttattgtctcatgagcggatacatatttgaatgtatttaga
aaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacct
gaaattgtaaacgttaatgttttgttaaatttcgcgttaaatatttgtta
aatcagcttattttttaaccagtaagcagaaaatgacaaaaatccttata
aatcaaaagaatagaccgagttagttgtgagtgttgttccagtttggaac
aagagtccactattaaagaacgtggactccaacgtaaaaccgtctatcag
ggcgatggcccactacgtgaaccatcacccaaatcaagtttttggaggtc
gaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgattta
gagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaa
gcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcg
cgtaaccaccacacccgccgcgcttaatgcgccgctactgggcgcgt
pUC19
Name: pUC19
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: New England Biolabs
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag
gtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
Full Sequence:
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag
gtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg
caccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcc
tgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcata
tggtgcactctcagtacaatctgctctgatgccgcatagttaagccagcc
ccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcc
cggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgt
cagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgt
gatacgcctatttttataggttaatgtcatgataataatggtttcttaga
cgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgttta
tttttctaaatacattcaaatatgtatccgctcatgagacaataaccctg
ataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatt
tccgtgtcgcccttattcccttttttgcggcattttgccttcctgttttt
gctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttggg
tgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttg
agagttttcgccccgaagaacgttttccaatgatgagcacttttaaagtt
ctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaact
cggtcgccgcatacactattctcagaatgacttggttgagtactcaccag
tcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagt
gctgccataaccatgagtgataacactgcggccaacttacttctgacaac
gatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatc
atgtaactcgccttgatcgttgggaaccggagctgaatgaagccatacca
aacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcg
caaactattaactggcgaactacttactctagcttcccggcaacaattaa
tagactggatggaggcggataaagttgcaggaccacttctgcgctcggcc
cttccggctggctggtttattgctgataaatctggagccggtgagcgtgg
gtctcgcggtatcattgcagcactggggccagatggtaagccctcccgta
tcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaat
agacagatcgctgagataggtgcctcactgattaagcattggtaactgtc
agaccaagtttactcatatatactttagattgatttaaaacttcattttt
aatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaa
atcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaa
gatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgct
tgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaa
gagctaccaactctttttccgaaggtaactggcttcagcagagcgcagat
accaaatactgttcttctagtgtagccgtagttaggccaccacttcaaga
actctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtg
gctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacg
atagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgca
cacagcccagcttggagcgaacgacctacaccgaactgagatacctacag
cgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacag
gtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttc
cagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctc
tgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatg
gaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggc
cttttgctcacatgttctttcctgcgttatcccctgattctgtggataac
cgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgac
cgagcgcagcgagtcagtgagcgaggaagcggaaga
pUC19-Cam
Name: pUC19-Cam
Antibiotic: chloramphenicol
Concentration: 25 ug/ml
Source: Takara Bio Inc.
Vector type: plasmid
Comments: Vector map previously at: http://catalog.takara-bio.co.jp/en/product/basic_info.asp?catcd=B1000503&subcatcd=B1000509&unitid=U100005395
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag
gtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
Full Sequence:
tcgacggagttccactgagcgtcagaccccgtagaaaagatcaaaggatc
ttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaa
aaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaact
ctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgt
ccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcac
cgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagt
ggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccgga
taaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagct
tggagcgaacgacctacaccgaactgagatacctacagcgtgagcattga
gaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaag
cggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacg
cctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgt
cgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccag
caacgcggcctttttacggttcctggccttttgctggccttttgctcaca
tgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcc
tttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcga
gtcagtgagcgaggaagcggaagaagctcattcgccattcaggctgcgca
actgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctg
gcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggtt
ttcccagtcacgacgttgtaaaacgacggccagtgaattcgagctcggta
ccccatcgatgggggatcctctagagtcgacccctcgaggggcatgcaag
cttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgc
tcacaattccacacaacatacgagccggaagcataaagtgtaaagcctgg
ggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcc
cgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggcc
aacgcgcggggagaggcggtttgcgtattggagcttggcactgggccaag
ctgaatttctgccattcatccgcttattatcacttattcaggcgtagcac
caggcgtttaagggcaccaataactgccttaaaaaaattacgccccgccc
tgccactcatcgcagtactgttgtaattcattaagcattctgccgacatg
gaagccatcacagacggcatgatgaacctgaatcgccagcggcatcagca
ccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaag
aagttgtccatattggccacgtttaaatcaaaactggtgaaactcaccca
gggattggctgagacgaaaaacatattctcaataaaccctttagggaaat
aggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtaga
aactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttc
agtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatca
ccagctcaccgtctttcattgccatacgaaattccggatgagcattcatc
aggcgggcaagaatgtgaataaaggccggataaaacttgtgcttattttt
ctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttat
aggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgc
cattgggatatatcaacggtggtatatccagtgatttttttctccatttt
agcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggta
gtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatca
acgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagg
gacaccaggatttatttattctgcgaagtgatcttccgt
pUC19-Kan
Name: pUC19-Kan
Antibiotic: kanamycin
Concentration: 50-200 ug/ml
Source: New England Biolabs
Vector type: plasmid
Comments: Vecor map previously at: http://catalog.takara-bio.co.jp/en/PDFFiles/3299_DS_e.pdf
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag
gtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
Full Sequence:
pUC19-Sfi
Name: pUC19-Sfi
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Codon Devices
Vector type: plasmid
Comments: pUC19-Sfi -- A pUC19 derivative with the multiple cloning site swapped with a SfiI linker. Vector map previously located at: http://catalog.takara-bio.co.jp/en/product/basic_info.asp?catcd=B1000503&subcatcd=B1000509&unitid=U100005395
Sequencing Primers: M13(-21), M13 reverse
Polylinker Sequence:
gaattcggccacattggccatctcactggcacagctccacctggcccggc
ctcttttggacggccttcgaggccggatcctctagagtcgacctgcaggc
atgcaagctt
Full Sequence:
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCG
GAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCG
TCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATG
CGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATA
CCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATT
CAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTAT
TACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTA
ACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATT
CGGCCACATTGGCCATCTCACTGGCACAGCTCCACCTGGCCCGGCCTCTT
TTGGACGGCCTTCGAGGCCGGATCCTCTAGAGTCGACCTGCAGGCATGCA
AGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCC
GCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCT
GGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTG
CCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG
CCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCT
CGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCA
GCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC
AGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCAT
CACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATA
AAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGC
GTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT
CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACAC
GACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAG
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACC
TTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGG
TAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGG
AACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGAT
CTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG
GCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACT
CCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCA
GTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCA
GCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAAC
TTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAA
GTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGC
ATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTC
CCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGG
TTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTG
TTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCC
ATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCT
GAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGG
GATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAA
ACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCA
GTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACT
TTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAA
AAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTT
TTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATAC
ATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATT
TCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACAT
TAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC
pUC57
Name: pUC57
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Genscript
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13 reverse, M13 -20
Full Sequence:
tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccg
gagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccg
tcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatg
cggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaata
ccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccatt
caggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctat
tacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggta
acgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaatt
cgagctcggtacctcgcgaatgcatctagatatcggatcccgggcccgtc
gactgcagaggcctgcatgcaagcttggcgtaatcatggtcatagctgtt
tcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccg
gaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcaca
ttaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtg
ccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgta
ttgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt
cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc
cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagc
aaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagg
ctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg
gcgaaacccgacaggactataaagataccaggcgtttccccctggaagct
ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc
gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtag
gtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacg
aaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt
gagtccaacccggtaagacacgacttatcgccactggcagcagccactgg
taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttga
agtggtggcctaactacggctacactagaagaacagtatttggtatctgc
gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagc
agattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttct
acggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt
catgagattatcaaaaaggatcttcacctagatccttttaaattaaaaat
gaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagt
taccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcg
ttcatccatagttgcctgactccccgtcgtgtagataactacgatacggg
agggcttaccatctggccccagtgctgcaatgataccgcgagacccacgc
tcaccggctccagatttatcagcaataaaccagccagccggaagggccga
gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaatt
gttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaac
gttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtat
ggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccc
ccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtc
agaagtaagttggccgcagtgttatcactcatggttatggcagcactgca
taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtg
agtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgc
tcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttt
aaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaagga
tcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaac
tgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaac
aggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgtt
gaatactcatactcttcctttttcaatattattgaagcatttatcagggt
tattgtctcatgagcggatacatatttgaatgtatttagaaaaataaaca
aataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaag
aaaccattattatcatgacattaacctataaaaataggcgtatcacgagg
ccctttcgtc
pYX-Asc
Name: pYX-Asc
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Columbia University/Soares
Vector type: plasmid
Has Custom Primer: yes
Vector Map: Available Here
Sequencing Primers: t7, t3, custom
Polylinker Sequence:
attaattaaccctcactaaagggacctaactataacggtcctaaggtagc
gagcggccgcggccagggaggccgatatcgaattcggcgcgccgtcgact
ttcattacctctttctccgcaccccacataaatctccctatagtgagtcg
tattaacatg
Full Sequence:
attaattaaccctcactaaagggacctaactataacggtcctaaggtagc
gagcggccgcggccagggaggccgatatcgaattcggcgcgccgtcgact
ttcattacctctttctccgcaccccacataaatctccctatagtgagtcg
tattaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg
ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcac
aaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaag
ataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccga
ccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtg
gcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgt
tcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct
gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgac
ttatcgccactggcagcagccactggtaacaggattagcagagcgaggta
tgtaggcggtgctacagagttcttgaagtggtggcctaactacggctaca
ctagaagaacagtatttggtatctgcgctctgctgaagccagttaccttc
ggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtag
cggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat
ctcaagaagatccttaccaatgcttaatcagtgaggcacctatctcagcg
atctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgatac
cgcgagacccacgctcaccggctccagatttatcagcaataaaccagcca
gcwggaagggccgagcgcagaagtggtcctgcaactttatccgcctccat
ccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta
atagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgc
tcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcg
agttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtc
ctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggtt
atggcagcactgcataattctcttactgtcatgccatccgtaagatgctt
ttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgc
ggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgcca
catagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcg
aaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccca
ctcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttct
gggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggc
gacacggaaatgttgaatactcatactcttcctttttcaat
pZERO-2
Name: pZERO-2
Antibiotic: kanamycin
Concentration: 25 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
ggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaa
acagctatgaccatgattacgccaagctatttaggtgacactatagaata
ctcaagctatgcatcaagcttggtaccgagctcggatccactagtaacgg
ccgccagtgtgctggaattctgcagatatccatcacactggcggccgctc
gagcatgcatctagagggcccaattcgccctatagtgagtcgtattacaa
ttcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta
cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaat
agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctata
Full Sequence:
agcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagctcactcattaggcaccccaggctttacact
ttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacaggaaacagctatgaccatgattacgccaagctatttaggtgaca
ctatagaatactcaagctatgcatcaagcttggtaccgagctcggatcca
ctagtaacggccgccagtgtgctggaattctgcagatatccatcacactg
gcggccgctcgagcatgcatctagagggcccaattcgccctatagtgagt
cgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaac
cctggcgttacccaacttaatcgccttgcagcacatccccctttcgccag
ctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgc
gcagcctatacgtacggcagtttaaggtttacacctataaaagagagagc
cgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggg
gcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaag
tctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgc
atgatgaccaccgatatggccagtgtgccggtctccgttatcggggaaga
agtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacc
tgatgttctggggaatataaatgtcaggcctgaatggcgaatggacgcgc
cctgtagcggcgcattaagcgcgcgggtgtggtggttacgcgcagcgtga
ccgctacacttgccagcgccctagcgcccgctcctttcgctttcttccct
tcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggg
gctccctttagggttccgatttagagctttacggcacctcgaccgcaaaa
aacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacg
gtttttcgccctttgacgttggagtccacgttctttaatagtggactctt
gttccaaactggaacaacactcaaccctatcgcggtctattcttttgatt
tataagggatgttgccgatttcggcctattggttaaaaaatgagctgatt
taacaaaaattttaacaaaattcagaagaactcgtcaagaaggcgataga
aggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaag
cggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaa
cgctatgtcctgatagcggtccgccacacccagccggccacagtcgatga
atccagaaaagcggccattttccaccatgatattcggcaagcaggcatcg
ccatgggtcacgacgagatcctcgccgtcgggcatgctcgccttgagcct
ggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcat
cctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcga
tgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcag
ccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggt
gagatgacaggagatcctgccccggcacttcgcccaatagcagccagtcc
cttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgt
cgtggccagccacgatagccgcgctgcctcgtcttgcagttcattcaggg
caccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgac
agccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtc
atagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatc
catcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatcagat
cttgatcccctgcgccatcagatccttggcggcgagaaagccatccagtt
tactttgcagggcttcccaaccttaccagagggcgccccagctggcaatt
ccggttcgcttgctgtccataaaaccgcccagtctagctatcgccatgta
agcccactgcaagctacctgctttctctttgcgcttgcgttttcccttgt
ccagatagcccagtagctgacattcatccggggtcagcaccgtttctgcg
gactggctttctacgtgaaaaggatctaggtgaagatcctttttgataat
ctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcaga
ccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcg
taatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgt
ttgccggatcaagagctaccaactctttttccgaaggtaactggcttcag
cagagcgcagataccaaatactgtccttctagtgtagccgtagttaggcc
accacttcaagaactctgtagcaccgcctacatacctcgctctgctaatc
ctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggtt
ggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacgg
ggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactg
agatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggag
aaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgca
cgagggagcttccagggggaaacgcctggtatctttatagtcctgtcggg
tttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggg
gcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctgg
gcttttgctggccttttgctcacatgttctttcctgcgttatcccctgat
tctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccg
cagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaag
pZL-1
Name: pZL-1
Antibiotic: ampicillin
Concentration: 50-200 ug/ml
Source: Invitrogen Life Technologies
Vector type: plasmid
Vector Map: Available Here
Comments: pSPORT plus a single LoxP site
Sequencing Primers: M13(-21), M13 reverse, t7, sp6
Polylinker Sequence:
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagcggccgccgactagtgagc
tcgtcgacccgggaattccggaccggtacctgcaggcgtaccagctttcc
Full Sequence:
cattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggc
ctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat
taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacg
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagcggccgccgactagtgagc
tcgtcgacccgggaattccggaccggtacctgcaggcgtaccagctttcc
ctatagtgagtcgtattagagcttggcgtaatcatggtcatagctgtttc
ctgtgtgaaattgttatccgctcacaattccacacaacatacgagccgga
agcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacatt
aattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgcc
agctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt
gggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgat
tgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctg
gtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggat
ataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccg
caccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgcc
atctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcag
catttgcatggtttgttgaaaaccggacatggcactccagtcgccttccc
gttccgctatcggctgaatttgattgcgagtgagatatttatgccagcca
gccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgc
gatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtac
cgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagaca
tcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggc
atcctggtcatccagcggatagttaatgatcagcccactgacccgttgcg
cgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttct
accatcgacaccaccacgctggcacccagttgatcggcgcgagatttaat
cgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaa
cgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttg
ggaatgtaattcagctccgccatcgccgcttccactttttcccgcgtttt
cgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataag
agacaccggcatactctgcgacatcgtataacgttactggtttcacattc
accaccctgaattgactctcttccgggcgctatcatgccataccgcgaaa
ggttttgcgccattcgatggtgtcaacgtaaatgccgcttcgccttcgcg
cgcgaattgcaagctctgcattaatgaatcggccaacgcgcggggagagg
cggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgc
gctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggta
atacggttatccacagaatcaggggataacgcaggaaagaacatgtgagc
aaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgt
ttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctca
agtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcc
ccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccg
gatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctggg
ctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggta
actatcgtcttgagtccaacccggtaagacacgacttatcgccactggca
gcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctac
agagttcttgaagtggtggcctaactacggctacactagaaggacagtat
ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggt
agctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgt
ttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctt
tgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaa
gggattttggtcatgccataacttcgtatagcatacattatacgaagtta
tggcatgagattatcaaaaaggatcttcacctagatccttttaaattaaa
aatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgac
agttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatt
tcgttcatccatagttgcctgactccccgtcgtgtagataactacgatac
gggagggcttaccatctggccccagtgctgcaatgataccgcgagaccca
cgctcaccggctccagatttatcagcaataaaccagccagccggaagggc
cgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatta
attgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgc
aacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttgg
tatggcttcattcagctccggttcccaacgatcaaggcgagttacatgat
cccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgtt
gtcagaagtaagttggccgcagtgttatcactcatggttatggcagcact
gcataattctcttactgtcatgccatccgtaagatgcttttctgtgactg
gtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagt
tgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaac
tttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaa
ggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcaccc
aactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaa
aacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaat
gttgaatactcatactcttcctttttcaatattattgaagcatttatcag
ggttattgtctcatgccaggggtgggcacacatatttgataccagcgatc
cctacacagcacataattcaatgcgacttccctctatcgcacatcttaga
cctttattctccctccagcacacatcgaagctgccgagcaagccgttctc
accagtccaagacctggcatgagcggatacatatttgaatgtatttagaa
aaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctg
aaattgtaaacgttaatattttgttaaaattcgcgttaaatttttgttaa
atcagctcattttttaaccaataggccgaaatcggcaaaatcccttataa
atcaaaagaatagaccgagatagggttgagtgttgttccagtttggaaca
agagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaacc
gtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttt
tttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcc
cccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaa
gggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggt
cacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagg
gcgcgtc