System Biosciences
AAVanced™ Concentration Reagent (250 ml aliquot)
- SKU:
- AAV110A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 250 ml
- Shipping Temperature:
- RT or Blue Ice
Description
AAVancedTM Concentration Reagent (250 ml aliquot). Cat# AAV110A. Supplier: SBI System Biosciences
- Easy to use, single reagent
- Isolate rAAV directly from media
- No cell lysates or centrifugation
- Save time and cost-effective
- Compatible with all serotypes
Products

Overview
- Easy to use, single reagent
- Isolate rAAV directly from media
- No cell lysates or centrifugation
- Save time and cost-effective
- Compatible with all serotypes
Unlike conventional AAV isolation methods which are labor-intensive, multi-step, and require cell lysis, CsCl2 density gradient ultracentrifugation, and chromatography, SBI’s AAVanced™ Concentration Reagent isolates rAAV particles from media in a single easy step. Simply add AAVanced Concentration Reagent to AAV particle-containing media, incubate overnight at 4°C, and then spin at 1,500g for thirty minutes.
Wash the pellet once, spinning again for only three minutes, and then resuspend and aliquot the concentrated virus. Titers of the isolated AAV particles are similar to titers of AAV particles isolated with traditional methods (Figure 1).
Figure 1. AAVanced Concentration Reagent delivers AAV particle preps with similar titers as AAV particles prepared using conventional methods. rAAV was packaged with a PGK-GFP expression shuttle vector. The rAAV particles were isolated from packaging cell media either by the conventional method—cell lysis freeze-thaw with density gradient ultracentrifugation—or with the AAVanced Concentration Reagent.Equal amounts of rAAV were then added to HEK293 cells to test for infectivity based on GFP expression. Representative images of GFP expressing cells 10-days after infection show similar levels of infectivity.
How It Works
Delivering a streamlined workflow for easier gene therapy and other AAV projects
Supporting Data
Demonstrably high titer with rAAV isolated using AAVanced Concentration Reagent
Figure 2. rAAV particles isolated using AAVanced Concentration Reagent are present at high copy numbers in 293T cells. A small volume of the rAAV virus isolated using AAVanced Concentration Reagent was used to infect 3×105 293T cells in a 24-well culture dish. The cells were imaged after 72-hours and rAAV copy numbers quantitated by qPCR. The copy numbers measured for the PGK-GFP rAAV virus were quite high at 2×1013 copies/mL.
Figure 3. AAVanced Concentration Reagent works with multiple serotypes in vitro. The same rAAV shuttle vector with a PGK promoter driving GFP expression was packaged in parallel into three different AAV serotypes: AAV-2, AAV-DJ and AAV-5. After 48-hours, media was collected and rAAV particles isolated using the AAVanced Concentration Reagent.
Each rAAV particle pellet was resuspended in 100 mL of sterile PBS, and then 1 mL of the rAAV suspensions were added to three different cell lines (HEK293T, HT1080 and CHO) to validate transduction efficiencies. The infected cells were imaged for GFP expression 6-days after addition of the isolated rAAV particles. All serotypes of rAAV were able to infect the three different cell types tested, demonstrating the broad compatibility of AAVanced Concentration Reagent.
Figure 4. rAAV particles isolated using AAVanced Concentration Reagent work in vivo. (Upper panels)
Representative images of hippocampal and midbrain sections from 6-week old C57 mice injected with AAV virus (ITR-PGK-GFP-ITR) concentrated using AAVanced Concentration Reagent. Approximately 1.5 µl of concentrated AAV virus (AAV-2 serotype) was delivered to hippocampal and midbrain regions by stereotaxic injection. Three weeks after virus injection, the animals were perfused with paraformaldehyde, fixed, and brains specimen were sectioned to 40 micron slices before imaging. The presence of robust GFP expression demonstrates successful delivery of the rAAV vector.
(Lower panels)
Representative images of sections of mouse CA3 neurons in the hippocampus from 2-month old C57 mice injected with AAV-5 virus (ITR-PGK-GFP-ITR) concentrated using AAVanced Concentration Reagent. Approximately 1.5 µl of concentrated rAAV-5 virus was delivered to cortical regions by stereotaxic injection. Two-weeks after virus injection, animals were perfused by paraformaldehyde, fixed and brains were sectioned to 70 micron slices before imaging. The presence of robust GFP expression demonstrates successful delivery of the rAAV vector.
Related Products

pSET152- high concentration | PVT11338
Nova Lifetech


AAVanced™ Concentration Reagent (100 ml aliquot)
System Biosciences


ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)
System Biosciences


pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1)
System Biosciences

Adeno-2™ pShuttle-His-x18 Cloning Kit | A250
ABM Good
Customers Also Viewed


AAVanced™ Concentration Reagent (100 ml aliquot)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)
System Biosciences
![AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro] AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12430/12694/sbi%20system%20biosciences_1633075021__00205.original__14894.1633076526.jpg?c=1)
![AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro] AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12430/15409/GE622A-1__14750.1638688062.png?c=1)
AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro]
System Biosciences

Ultra-high Titer, 200 µL (> 1 x 10^9 IFU/mL) | 568-S501UL
101Bio

Ultra-high Titer, 100 µL (> 1 x 10^9 IFU/mL) | 568-S501U
101Bio

High titer, 200 µL (> 1 x 10^8 IFU/mL) | 568-S501HL
101Bio

AAV Packaging Mix (Serotype 4) & DNAfectin Combo Pack | AAV1004-G2100
ABM Good

AAV Packaging Mix (Serotype 4) | AAV1004
ABM Good

AAV Packaging Mix (Serotype 3) | AAV1003
ABM Good

AAV Packaging Mix (Serotype 2) | AAV1002
ABM Good

AAV Packaging Mix (Serotype 1) & DNAfectin Combo Pack | AAV1001-G2100
ABM Good


pAAVK-EF1α-MCS1-CMV-Puro AAVanced™ Cloning and Expression Vector
System Biosciences


pAAVK-EF1α-MCS1-CMV-EGFP AAVanced™ Cloning and Expression Vector
System Biosciences


pAAVK-EF1α -MCS AAVanced™ Cloning and Expression Vector
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


Exosome-Depleted FBS Media Supplement
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP)
System Biosciences


AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
System Biosciences
![AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro] AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12431/12695/sbi%20system%20biosciences_1633075021__00205.original__65072.1633076526.jpg?c=1)
![AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro] AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/12431/15410/PIN610A-1__11136.1638689071.png?c=1)
AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro]
System Biosciences


293TN Producer Cell Line
System Biosciences

High titer Large, 200 µL (> 1 x 10^8 IFU/mL) | 568-S502HL
101Bio

Exosome size distribution analysis (NTA) | 568-S108
101Bio

Exosome Biotin labeling | 568-S106
101Bio

Disease cell exosome isolation | 568-S102
101Bio

Custom cell exosome isolation | 568-S101
101Bio

Lyophilized Exosomes from Human Saliva of Healthy Donors | PESL
HBM

Lyophilized Exosomes from Human Serum of Healthy Donors | PES
HBM

Hsp70-Firefly Luc (GFP) Lentiviral particles | LVP865-G
GenTarget

ARHGEF7 Clone BC060776 pUC
Cusabio

ARHGEF7 Clone BC033905 pUC
Cusabio

Plasma Membrane Labeling Lentivirus (Farnesyl Signal)(CFP) | LVP711
ABM Good

Plasma Membrane Labeling Lentivirus (Farnesyl Signal)(RFP) | LVP710
ABM Good

AAV Packaging Mix (Serotype 6) & DNAfectin Combo Pack | AAV1006-G2100
ABM Good

AAV Packaging Mix (Serotype 5) & DNAfectin Combo Pack | AAV1005-G2100
ABM Good

AAV Packaging Mix (Serotype 5) | AAV1005
ABM Good

AAV Packaging Mix (Serotype 1) | AAV1001
ABM Good

3rd Gen. Packaging Mix & Lentifectin Combo Pack | LV053-G074
ABM Good

2nd Gen. Packaging Mix & Lentifectin Combo Pack | LV003-G074
ABM Good

qPCR Adeno-Associated Virus Titration (Titer) Kit | G931
ABM Good

pShuttle-2A-RFP Control Vector | A022
ABM Good

Adeno-8™ pShuttle-2A-RFP Cloning Kit | A810
ABM Good

Adeno-8™ pShuttle-2A-RFP Complete Cloning Kit | A800
ABM Good

Adeno-2™ pShuttle-His-x18 Cloning Kit | A250
ABM Good

Adeno-2™ pShuttle-His-18x Complete Cloning Kit | A240
ABM Good

Add-N-Pure™ Adenovirus Purification Kit (2 GigaU) | A910
ABM Good

Add-N-Pure™ Adenovirus Purification Kit (5 U) | A905
ABM Good

Add-N-Pure™ Adenovirus Purification Kit (2 U) | A902
ABM Good

Adeno-5™ pLuc Cloning Kit | A510
ABM Good