System Biosciences

CAGs-GFP-T2A-Luciferase Dual Reporter Enhanced Episomal Vector (EEV), 10 ug (Replaces EEV604A-1)

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SKU:: EEV604A-2
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Availability:: Usually Shipped in 5 Working Days
Size:: 10 ug
Shipping Temperature:: Blue Ice/ Dry Ice

€791.00

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Description

Description

CAGs-GFP-T2A-Luciferase Dual Reporter Enhanced Episomal Vector (EEV), 10 ug (Replaces EEV604A-1) . Cat# EEV604A-2. Supplier: SBI System Biosciences

Get sustained, non-viral constitutive expression of GFP and luciferase reporters with this EEV plasmid and avoid the challenges of viral transduction

High levels of expression
Easy to clone formats
No special plasmid production
Nonviral, non-integrating technology
Constitutive and inducible vector formats

Products

Overview

An easy-to-produce non-integrating option for constitutive GFP and luciferase expression With virtually no limits on insert size (unlike AAV vectors) Enhanced Episomal Vectors (EEVs) are an excellent choice for non-integrating, non-viral gene expression. Because they replicate in synchrony with the host cell, they are stably inherited and can be used for long-lasting expression—up to several months both in vitro and in vivo—without modifying the host genome. Use SBI’s CAGs-GFP-T2A-Luciferase EEV Vector (Cat.# EEV604A-2) for constitutive, CAG-driven coordinated expression of GFP and luciferase reporters in most cell types, including primary cells and stem cells.

SBI’s EEV System, which is an enhanced version of the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA1) system, offers:

High levels of expression
Easy to clone formats
No special plasmid production
Nonviral, non-integrating technology
Constitutive and inducible vector formats

Supporting Data

Sustained, non-integrating gene expression in vitro and in vivo with EEV

Figure 1. A constitutive EEV reporter based on CAGs-MCS delivers similar amounts of GFP expression in vitro after two days and eleven days.

SBI’s EEV reporter plasmid CAGs-GFP-Luciferase (Cat.# EEV604A-2), contains a GFP-T2A-Luciferase cassette cloned into the CAGs-MCS EEV Vector (Cat.# EEV601A-1). This plasmid was transfected into HEK293T cells (0.5 µg of CAGs-GFP-Luciferase in a 24-well plate) and GFP transgene expression imaged. There is little change in GFP signal between two- and eleven-days post-transfection.

Figure 2. A constitutive EEV reporter based on CAGs-MCS delivers high levels of expression in mice over forty days. 8 µg of the constitutive CAGs-GFP-T2A-Luc construct (Cat.# EEV604A-2) was introduced into test mice through hydrodynamic tail vein injection (HDD), a procedure which leads to high plasmid DNA transfection of the livers of mice in vivo. The test mice (n=3) were imaged for body luminescence in the liver area post-HDD from Day 1 up until Day 80. The results show that robust EEV-expressed luciferase expression is readily detectable at very high levels through day 40.