EVeryRNA™ EV RNA Purification System with ExoQuick EV Isolation (5 ml)
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EVeryRNA™ EV RNA Purification System with ExoQuick EV Isolation (5 ml). Cat# EVery106EQ. Supplier: SBI System Biosciences</
Discover more when you capture total EV RNA
Combine the time- and labor-savings of ExoQuick EV Isolation technology with the total RNA isolation capabilities of EVeryRNA EV RNA Purification technology for a complete solution for your EV RNA biomarker workflows.The EVeryRNA EV RNA Purification System overcomes many of the challenges faced when isolating RNA from extracellular vesicles (EVs), most notable is the ability to capture total EV RNA, including small RNAs. EVeryRNA is effective even with low amounts of input RNA and is capable of delivering high yields of highly pure RNA. Because the RNA elutes in a small sample volume, thus generating a highly concentrated prep, you can increase the amount of RNA used in a single downstream reaction for better data coverage quality.
- Move quickly and confidently with exoRNA isolation that's high-yield and complete in <30 minutes
- Find what others miss when you capture every RNA with EVeryRNA
- Achieve phenol-level yields with a safer column-based method
- Get more RNA for each downstream reaction with EVeryRNA's small-volume elutions
- Isolate RNA from EVs for a full range of downstream applications, such as RNA-seq and miRNA profiling
- Ensure delivery of highly pure RNA by using the included DNaseI
- Maximize productivity with ExoQuick EV Isolation bundled with EVeryRNA
Table 1. EVeryRNA EV RNA Purification Products
How It Works
Maximize productivity with ExoQuick and EVeryRNA
Isolate EVs with ExoQuick
Isolating EVs from plasma, serum, and ascites fluid with ExoQuick is quick and easy, with a simple workflow that involves minimal hands-on time and low input sample volume requirements (Figure 1). ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.
To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:
- Add an appropriate volume of ExoQuick to as little as 100 µl sample
- Incubate for at least one hour at 4°C
- Isolate exosomes with a 30-minute low-speed spin (1500 g).
Purify EV RNA with EVeryRNAThe EVeryRNA EV RNA Purification System delivers high yields of highly concentrated RNA from already isolated EVs. The column-based workflow is easy to implement and can be completed in less than 30 minutes (Figure 2).
Characterizing ExoQuick EVs with NanoSightEVs purified with ExoQuick from serum show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 3).
EVeryRNA captures EVerythingTo demonstrate the ability of the EVeryRNA EV RNA Purification System to capture the full range of RNAs, we used the kit to isolate RNA from 10,000 cells (Figure 4, lane 1), from EVs that were isolated from 250 µL of serum using SmartSEC Single (Figure 4, lane 2), and from buffer spiked with 0.1 pmol of Cel-miR-39 (Figure 4, lane 3). The high quality of the isolated RNA can be seen in lane 1, where the RNA integrity number (RIN) is 9.9 and the 28S/18S RNA ratio is 1.5. The multiple bands in lane 2 demonstrate that EVeryRNA captures RNAs of different lengths—EVerything—from EVs with no apparent bias or size preference. The strong signal from the spiked-in miRNA in lane 3 demonstrates the good recovery of even small RNAs.
EVeryRNA delivers similar amounts of RNA as phenol-based methodsTo demonstrate the excellent RNA yields obtained with EVeryRNA, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both EVeryRNA and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVeryRNA cDNA Synthesis & Pre-amplification Kit and the copy number of Cel-miR-39 measured (Figure 6). The EVeryRNA EV RNA Purification System delivered similar levels of Cel-miR-39 as the phenol-based method.
EVeryRNA efficiently isolates mRNAWe used the EVeryRNA EV RNA isolation kit to isolate mRNA from cells overexpressing eGFP (Figure 7). Robust levels of eGFP mRNA are recovered when cells are overexpressing eGFP.
miRNA isolated using EVeryRNA can be used for miRNA profiling and RNA-seqWe isolated EVs from 250 µL of serum using SmartSEC Single and used the EVeryRNA EV RNA Purification System and EVeryRNA cDNA Synthesis & Pre-amplification System to isolate and reverse transcribe EV RNAs for miRNA profiling using the SeraMir Human Exosome RNA Profiling Plate. We were able to detect a number of miRNAs both with amplification (96 miRNAs) and without amplification (16 miRNAs, with 14 overlapping with the miRNAs detected with amplification, Figure 8).