EVeryRNA™ EV RNA Purification System
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EVeryRNA™ EV RNA Purification System. Cat# EVery100A. Supplier: SBI System Biosciences</
- Move quickly and confidently with exoRNA isolation that's high-yield and complete in <30 minutes.
- Find what others miss when you capture every RNA with EVeryRNA
- Achieve phenol-level yields with a safer column-based method
- Get more RNA for each downstream reaction with EVeryRNA's small-volume elutions
- Ensure delivery of highly pure RNA by using the included DNaseI
- Compatible with most downstream applications, including RNA-seq and miRNA profiling
- Works with EVs isolated with commonly-used methods, such as ExoQuick, SmartSEC, and ultracentrifugation.
Table 1. EVeryRNA EV RNA Purification Products
How It Works
The EVeryRNA EV RNA Purification System delivers high yields of highly concentrated RNA from already isolated EVs. The column-based workflow is easy to implement and can be completed in less than 30 minutes (Figure 1).
To demonstrate the ability of the EVeryRNA EV RNA Purification System to capture the full range of RNAs, we used the kit to isolate RNA from 10,000 cells (Figure 2, lane 1), from EVs that were isolated from 250 µL of serum using SmartSEC Single (Figure 2, lane 2), and from buffer spiked with 0.1 pmol of Cel-miR-39 (Figure 2, lane 3). The high quality of the isolated RNA can be seen in lane 1, where the RNA integrity number (RIN) is 9.9 and the 28S/18S RNA ratio is 1.5. The multiple bands in lane 2 demonstrate that EVeryRNA captures RNAs of different lengths—EVerything—from EVs with no apparent bias or size preference. The strong signal from the spiked-in miRNA in lane 3 demonstrates the good recovery of even small RNAs.
The compatibility of multiple EV isolation techniques with EVeryRNA and the excellent size distribution of RNAs isolated from those EVs is shown in Figure 3.
EVeryRNA delivers similar amounts of RNA as phenol-based methods
To demonstrate the excellent RNA yields obtained with EVeryRNA, we isolated EVs from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both EVeryRNA and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVeryRNA cDNA Synthesis & Pre-amplification Kit and the copy number of Cel-miR-39 measured (Figure 4). The EVeryRNA EV RNA Purification System delivered similar levels of Cel-miR-39 as the phenol-based method.
EVeryRNA efficiently isolates mRNA
We used the EVeryRNA EV RNA isolation kit to isolate mRNA from cells overexpressing eGFP (Figure 5). Robust levels of eGFP mRNA are recovered when cells are overexpressing eGFP.
miRNA isolated using EVeryRNA can be used for miRNA profiling and RNA-seq
We isolated EVs from 250 µL of serum using SmartSEC Single and used the EVeryRNA EV RNA Purification System and EVeryRNA cDNA Synthesis & Pre-amplification System to isolate and reverse transcribe EV RNAs for miRNA profiling using the SeraMir Human Exosome RNA Profiling Plate. We were able to detect a number of miRNAs both with amplification (96 miRNAs) and without amplification (16 miRNAs, with 14 overlapping with the miRNAs detected with amplification, Figure 6).
We were also able to show robust, successful RNA-seq runs using RNA isolated from EVs with EVeryRNA (Table 2). All three EV isolation methods tested generated high-quality RNA-seq data.
Table 2. Successful RNA-seq with EVeryRNA-isolated EV RNA