Exo-APC Exosome FACS stain

Visualize bead-immobilized exosomes with our proprietary, reversible APC-based stain

Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-APC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-APC is comprised of the commonly used fluorophore allophycocyanin (APC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.

However, the feature that really makes Exo-APC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-APC and flow-sorting by APC-positive gating (Exo-APC’s excitation and emission wavelengths are 650 nm and 660 nm, respectively), Exo-APC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-APC.

Note that we also offer reversible Exo-FITC (Cat.# Exo-FLOW800A-1) as an alternative stain to Exo-APC.