System Biosciences
Exo-APC Exosome FACS stain
- SKU:
- EXOBAC100A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 20 reactions
- Shipping Temperature:
- Blue Ice
Description
Exo-APC Exosome FACS stain. Cat# EXOFLOW810A. Supplier: SBI System Biosciences
Overview
Visualize bead-immobilized exosomes with our proprietary, reversible APC-based stain
Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-APC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-APC is comprised of the commonly used fluorophore allophycocyanin (APC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.
However, the feature that really makes Exo-APC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-APC and flow-sorting by APC-positive gating (Exo-APC’s excitation and emission wavelengths are 650 nm and 660 nm, respectively), Exo-APC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-APC.
Note that we also offer reversible Exo-FITC (Cat.# Exo-FLOW800A-1) as an alternative stain to Exo-APC.
Use Exo-APC with any of our Exo-Flow Capture Kits:
How It Works
Easily purify exosomes using FACS with our Exo-Flow Capture Kits
At a glance
Simply (1) couple biotinylated antibody to the magnetic streptavidin beads, (2) use the antibody-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-APC (excitation and emission wavelengths of 650 nm and 660 nm, respectively).
Your sample is now ready for FACS analysis.
To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-APC stain and elute intact exosomes from the beads.
Supporting Data
See Exo-APC in action
Exo-APC enables selective exosome isolation via FACS. Exosomes are isolated using the CD9 Exo-Flow Capture Kit and flow-sorted using APC-positive gating.
Related Products


Exo-FITC Exosome FACS stain
System Biosciences

Exo-FACS ready to use kit for analysis of human PLASMA exosomes | FACS-PEP
HBM

Exo-FACS ready to use kit for analysis of human URINE exosomes | FACS-PEU
HBM

Exo-FACS ready to use kit for analysis of human SALIVA exosomes | FACS-PESL
HBM

Exo-FACS ready to use kit for analysis of exosomes markers from cell media | FACS-C
HBM
Customers Also Viewed

YCp50 | PVT11247
Nova Lifetech

AAV Packaging Mix (Serotype 2) | AAV1002
ABMGood

Complete Kit for 2nd Generation miRNA Lentiviruses | MK001
ABMGood
![pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM virus] pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14447/14715/sbi%20system%20biosciences_1633075021__00205.original__89336.1633076844.jpg?c=1)
![pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM virus] pLL-CMV-GFP-T2A-Puro [Lenti-LabelerTM virus]](https://cdn11.bigcommerce.com/s-i3n9sxgjum/images/stencil/590x590/products/14447/29977/LL100VA-1__47320.1640242108.png?c=1)
pLL-CMV-GFP-T2A-Puro [Lenti-Labeler™ virus]
System Biosciences

miRZip-let7i anti-miR-let7i microRNA construct
System Biosciences

miRZip-let7g anti-miR-let7g microRNA construct
System Biosciences

miRZip-let7c anti-miR-let7c microRNA construct Bacterial streak
System Biosciences

miRZip-885-5p anti-miR-885-5p microRNA construct bacterial streak
System Biosciences

miRZip-653 anti-miR-653 microRNA construct
System Biosciences

miRZip-520c-3p anti-miR-520c-3p microRNA construct
System Biosciences

miRZip-497 anti-miR-497 microRNA construct
System Biosciences

miRZip-494 anti-miR-494 microRNA construct
System Biosciences

miRZip-486-5p anti-miR-486-5p microRNA construct
System Biosciences

miRZip-483-3p anti-miR-483-3p microRNA construct
System Biosciences

miRZip-429 anti-miR-429 microRNA construct
System Biosciences

miRZip-184 anti-miR-184 microRNA construct
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


ExoQuick TC 10 ml
System Biosciences


Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media
System Biosciences


Cas9 Nickase: CAG-hspCas9-nickase-H1-gRNA SmartNickase vector
System Biosciences

W303a(W303-1A) Plasmid | PVT13742
Nova Lifetech

VVT1 | PVT10930
Nova Lifetech

VEGFA Plasmid | PVT7127
Nova Lifetech

TRAF7 Plasmid | PVT18204
Nova Lifetech

Top10 Escherichia coli Strains | S0003
Nova Lifetech

TetO- FUW- OSKM | PVT11553
Nova Lifetech

RLN2 Plasmid | PVT7126
Nova Lifetech

pY010 (pCDNA3.1- hAsCpf1) | PVT10923
Nova Lifetech

pLJM1-EGFP Plasmid | PVT12367
Nova Lifetech

pGEM-SMURF1 Plasmid | PVTB00684
Nova Lifetech

pGEM-Ltf Plasmid | PVTB50084-1
Nova Lifetech

pGEM-hLRP6 Plasmid | PVTB00515
Nova Lifetech

pEYFP-N1-ABCB11(Y472C/D1131V) Plasmid | PVTB01041-2d
Nova Lifetech

pET-6His-MBP-MpAgo Plasmid | PVT17703
Nova Lifetech

pEnCMV-CCL15-3 Plasmid | PVT18253
Nova Lifetech

pEnCMV-AZGP1-3 Plasmid | PVT19095
Nova Lifetech

pcDNA3-H-Ras_V12 Plasmid | PVT15905
Nova Lifetech

pCDNA3.1-YAP1-3 Plasmid | PVT17419
Nova Lifetech

pCas9 Plasmid | PVT6317
Nova Lifetech

p3301/ LBA4404 Agrobacterium tumefaciens Strains | S0102
Nova Lifetech

ET12567 (pUZ8002) Escherichia coli Strains | S0052
Nova Lifetech

BL21pLysS (DE3) Escherichia coli Strains | S0005
Nova Lifetech