System Biosciences
Exo-FITC Exosome FACS stain
- SKU:
- EXOFLOW800A-1
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 20 reactions
- Shipping Temperature:
- Blue Ice
Description
Exo-FITC Exosome FACS stain. Cat# EXOFLOW800A. Supplier: SBI System Biosciences
Overview
Visualize bead-immobilized exosomes with our proprietary, reversible FITC-based stain
Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-FITC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-FITC is comprised of the commonly used fluorophore fluorescein isothiocyanate (FITC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.
However, the feature that really makes Exo-FITC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-FITC and flow-sorting by FITC-positive gating (Exo-FITC’s excitation and emission wavelengths are 494 nm and 518 nm, respectively), Exo-FITC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-FITC.
Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.
Use Exo-FITC with any of our Exo-Flow Capture Kits:
How It Works
Easily purify exosomes using FACS with our Exo-Flow Capture Kits
At a glance
Simply (1) couple biotinylated antibody to the magnetic streptavidin beads, (2) use the antibody-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).
Your sample is now ready for FACS analysis.
To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.
Supporting Data
See Exo-FITC in action
Exo-FITC enables selective exosome isolation via FACS. Exosomes are isolated using the CD9 Exo-Flow Capture Kit and flow-sorted using FITC-positive gating.
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