Exo-Flow96 CD63 IP kit
- Usually Shipped in 5 Working Days
- 96 reactions
- Shipping Temperature:
- Blue Ice
Exo-Flow96 CD63 IP kit. Cat# EXOFLOW96A-CD63. Supplier: SBI System Biosciences
Streamline affinity-based exosome immunopurification
With magnetic beads already pre-coupled to anti-CD63 antibodies and delivered in a 96-well format, SBI’s Exo-Flow96 CD63 IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD63 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproducible affinity-based exosome purification directly from serum or plasma.
Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC® or ultracentrifugation.
- Selection of well-validated antibodies for reliable and reproducible purification
- Large-sized magnetic beads increase the efficiency of exosome capture
- Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation
Different formats for different needsTo facilitate a range of studies, SBI built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.