Exo-Flow96 CD9 IP kit
- Usually Shipped in 5 Working Days
- 96 reactions
- Shipping Temperature:
- Blue Ice
Exo-Flow96 CD9 IP kit. Cat# EXOFLOW96A-CD9. Supplier: SBI System Biosciences
Streamline affinity-based exosome immunopurification
With magnetic beads already pre-coupled to anti-CD9 antibodies and delivered in a 96-well format, SBI’s Exo-Flow96 CD9 IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD9 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproducible affinity-based exosome purification directly from serum or plasma. Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC® or ultracentrifugation.
- Selection of well-validated antibodies for reliable and reproducible purification
- Large-sized magnetic beads increase the efficiency of exosome capture
- Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation
Even better, with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, enabling capture of billions of exosomes from one sample.
Each IP kit comes with all the necessary reagents for immunopurification—capture antibody pre-coupled to magnetic beads in 96-well clear plates (as 8-well strips) with covers, wash buffer, Exosome Elution Buffer, and clearing reagent (to remove Exosome Elution Buffer). A 96-well plate magnet, the Exo-FlowMag96, can be purchased separately (Cat.# EXOFLOWMAG-1).
Different formats for different needs
To facilitate a range of studies, SBI built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.
See isolation data using Exo-FLOW IP Kits
For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.
Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.
Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.