System Biosciences
HEK293 Human embryonic kidney cell line: >1x10^10 frozen exosomes
- SKU:
- CAS630A-1
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 50 ug
- Shipping Temperature:
- Dry Ice
Description
HEK293 Human embryonic kidney cell line: >1x10^10 frozen exosomes. Cat# EXOP10A. Supplier: SBI System Biosciences
Overview
How It Works
Genome engineering with CRISPR/Cas9
For general guidance on using CRISPR/Cas9 technology for genome engineering, take a look at our CRISPR/Cas9 tutorials as well as the following application notes:
CRISPR/Cas9 Gene Knock-Out Application Note (PDF) »
CRISPR/Cas9 Gene Editing Application Note (PDF) »
CRISPR/Cas9 Gene Tagging Application Note (PDF) »
CRISPR/Cas9 Basics
Through careful selection of the target sequence and design of a donor plasmid for homologous
recombination, you can achieve efficient and highly targeted genomic modification with CRISPR/Cas9.
The system
Cas9 protein—uses guide RNA (gRNA) to direct site-specific, double-strand DNA cleavage adjacent to a protospacer adapter motif (PAM) in the target DNA.
gRNA—RNA sequence that guides Cas9 to cleave a homologous region in the target genome. Efficient cleavage only where the gRNA homology is adjacent to a PAM.
PAM—protospacer adapter motif, NGG, is a target DNA sequence that spCas9 will cut upstream from if directed to by the gRNA.
The workflow at-a-glance
DESIGN: Select gRNA and HR donor plasmids. Choice of gRNA site and design of donor
plasmid determines whether the homologous recombination event results in a knock-out,
knock-in, edit, or tagging.
CONSTRUCT: Clone gRNA into all-in-one Cas9 vector. Clone 5’ and 3’ homology arms into HR
donor plasmid. If creating a knock-in, clone desired gene into HR donor.
CO-TRANSFECT or CO-INJECT: Introduce Cas9, gRNA, and HR Donors into the target cells
using co-transfection for plasmids, co-transduction for lentivirus, or co-injection for mRNAs.
SELECT/SCREEN: Select or screen for mutants and verify.
VALIDATE: Genotype or sequence putative mutants to verify single or biallelic conversion.
Related Products


A549 Human non-small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


DMS114 Human small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


H196 Human small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


PC-3 Human prostate cancer cells derived from metastatic cancer cell line: >1x10^10 frozen exosomes
System Biosciences

Lyophilized Exosomes from HEK293 Cell Line | HEK293
HBM
Customers Also Viewed


XStamp Pro Streptavidin Customizable EV Targeting Kit
System Biosciences


XStamp Pro anti-PD-1 EV Targeting Kit
System Biosciences


XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro
System Biosciences


XPack CMV-XP-MCS-EF1-Puro Cloning Lentivector
System Biosciences


XPack CMV-XP-Luciferase-EF1-Puro Expression Lentivector
System Biosciences


XPack CMV-XP-GFP-EF1-Puro Expression Lentivirus
System Biosciences


XCF Exosomal DNA Isolation Kit
System Biosciences


XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)
System Biosciences


Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL)
System Biosciences


SmartSEC™ Mini EV Isolation System
System Biosciences


SmartSEC™ HT EV Isolation System (for Serum & Plasma) - Trial Size
System Biosciences


SmartSEC™ HT EV Isolation System (for Serum & Plasma)
System Biosciences


SmartSEC Single EV Isolation System
System Biosciences


SeraMir Exosome RNA Purification kit (5ml ExoQuick and 20 exoRNA columns)
System Biosciences


RAWS 264.7 Mouse macrophage cell line: >1x10^6 frozen exosomes
System Biosciences


PC-3 Human prostate cancer cells derived from metastatic cancer cell line: >1x10^10 frozen exosomes
System Biosciences


H841 Human small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


H196 Human small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


Exosome-Depleted FBS Media Supplement Heat Inactivated
System Biosciences


Exosome-Depleted FBS Media Supplement
System Biosciences


ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media
System Biosciences


ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
System Biosciences


ExoQuick PLUS Exosome Purification Kit - for serum and plasma
System Biosciences


ExoQuick Exosome RNA Column Purification kit
System Biosciences


ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)
System Biosciences


ExoMS Surface Protein Capture Kit (Tissue Culture EVs)
System Biosciences


ExoMS Surface Protein Capture Kit (Serum/Plasma EVs)
System Biosciences


Exo-FITC Exosome FACS stain
System Biosciences


ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody
System Biosciences


EVeryRNA™ EV RNA Purification System with SmartSEC Single (10 reactions)
System Biosciences


EV Shuttle Kit Mouse JAWS II Mouse bone marrow dendritic cell exosomes (10 rxn)
System Biosciences


EV Shuttle Kit Human HEK293 cell exosomes (10 rxn)
System Biosciences


DMS114 Human small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences


BC-3 Human B lymphocyte cell line: >1x10^6 frozen exosomes
System Biosciences


A549 Human non-small cell lung cancer cell line: >1x10^10 frozen exosomes
System Biosciences