HIV & FIV Positive Transduction Control Virus Sampler Kit [pSIF1- and pSIH-H1-siLuc-copGFP lentiviruses]

Gain the confidence to move forward with informative controls

While lentiviral transduction can be a powerful and effective method for delivering expression cassettes to target cells, enabling stable expression of genes, miRNAs, shRNAs, etc., some cell lines are transduced less efficiently than others. To help with lentiviral transduction optimization and troubleshooting, SBI offers a series of positive transduction controls that come as pre-packaged, ready-to-transduce lentiviral particles as well as ready-to-transfect and package lentivector plasmids. The HIV & FIV Positive Transduction Control Virus Sampler Kit includes both HIV- and FIV-based Positive Transduction Controls—pSIH1-H1-siLuc-copGFP and pSIF1-H1-siLuc-copGFP, respectively, and enables quantitation and comparison of HIV- and FIV-based lentiviral transduction efficiencies using the bright copGFP protein.

• Easily visualize and quantify transduction efficiency with copGFP
• Optimize transduction conditions for your cell line
• Compare the sensitivity of target cells to HIV- versus FIV-based lentiviral transduction

Both pSIH1-H1-siLuc-copGFP and pSIF1-H1-siLuc-copGFP express the full-length copGFP gene with human-optimized codons from the CMV promoter for high levels of expression in mammalian cells. The copGFP marker is a novel natural green monomeric GFP-like protein from copepods (Pontellina sp.). The copGFP protein is a non-toxic, non-aggregating protein with fast protein maturation and high stability at a wide range of pH (pH 4 – 12). copGFP does not require any additional cofactors or substrates and has very bright fluorescence that exceeds at least 1.3 times the brightness of EGFP, the widely used Aequorea victoria GFP mutant. The copGFP protein emits green fluorescence with the following characteristics:

• Maximum emission wavelength: 502 nm
• Maximum excitation wavelength: 482 nm
• Quantum yield: 0.6
• Extinction coefficient: 70,000 M^-1 cm^-1